Mass spectrometric identification of Rab23 phosphorylation as a response to challenge by cytidine 3',5'-cyclic monophosphate in mouse brain.

While the functions and mechanisms of action of adenosine 3',5'-cyclic monophosphate (cAMP) and guanosine 3',5'-cyclic monophosphate (cGMP) are well established and are the basis of the action of a large number of successful pharmaceuticals, the role of a third naturally occurring cyclic nucleotide, cytidine 3',5'-cyclic monophosphate (cCMP), remains to be elucidated. Immobilized metal affinity chromatography (IMAC) was used to selectively extract proteins phosphorylated in mouse brain in response to challenge by cAMP, cGMP and cCMP, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToFMS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) of tryptic digests to identify Rab23 as the first protein reported to be phosphorylated only in response to cCMP.

[1]  J. Wikberg,et al.  Investigations on the occurrence of cytidine 3',5' monophosphate (cCMP) in tissues. , 2009, Acta pharmacologica et toxicologica.

[2]  J. Wikberg,et al.  Cytidine 3',5' Monophosphate (cCMP) is not an Endogenous nucleotide in normal or regenerating rat livers. , 2009, Acta pharmacologica et toxicologica.

[3]  Steven P Gygi,et al.  Comparative evaluation of mass spectrometry platforms used in large-scale proteomics investigations , 2005, Nature Methods.

[4]  G. Lubec,et al.  Expression of hypothetical proteins in human fetal brain: increased expression of hypothetical protein 28.5kDa in Down syndrome, a clue for its tentative role. , 2003, Molecular genetics and metabolism.

[5]  Stephen B. Baylin,et al.  Hedgehog signalling within airway epithelial progenitors and in small-cell lung cancer , 2003, Nature.

[6]  C. Hui,et al.  Dissecting the oncogenic potential of Gli2: deletion of an NH(2)-terminal fragment alters skin tumor phenotype. , 2002, Cancer research.

[7]  G. Chaga,et al.  Twenty-five years of immobilized metal ion affinity chromatography: past, present and future. , 2001, Journal of biochemical and biophysical methods.

[8]  V. Gaberc-Porekar,et al.  Perspectives of immobilized-metal affinity chromatography. , 2001, Journal of biochemical and biophysical methods.

[9]  K. Anderson,et al.  Rab23 is an essential negative regulator of the mouse Sonic hedgehog signalling pathway , 2001, Nature.

[10]  R. Newton.  Cytidine 3′,5′-Cyclic Monophosphate: A Third Cyclic Nucleotide Secondary Messenger? , 1995 .

[11]  R. Cooke,et al.  Radioimmunoassay of cytidine 3',5'-cyclic monophosphate: unambiguous assay by means of an optimized protocol incorporating a trilayer column separation to obviate cross-reactivity problems. , 1994, Journal of immunoassay.

[12]  J. Langridge,et al.  Quantitation by fast-atom bombardment mass spectrometry: assay of cytidine 3',5'-cyclic monophosphate-responsive protein kinase. , 1992, Rapid communications in mass spectrometry : RCM.

[13]  R. Newton,et al.  Cytidylate cyclase: development of assay and determination of kinetic properties of a cytidine 3',5'-cyclic monophosphate-synthesizing enzyme. , 1990, The Biochemical journal.

[14]  R. Newton,et al.  Cytidylate cyclase activity: identification of cytidine 3',5'-cyclic monophosphate and four novel cytidine cyclic phosphates as biosynthetic products from cytidine triphosphate. , 1988, Rapid communications in mass spectrometry : RCM.

[15]  R. Newton,et al.  Extraction, purification and identification of cytidine 3',5'-cyclic monophosphate from rat tissues. , 1984, The Biochemical journal.

[16]  T. Anderson.  Cyclic cytidine 3',5'-monophosphate (cCMP) in cell regulation , 1982, Molecular and Cellular Endocrinology.

[17]  D. Helfman,et al.  A homogeneous cyclic CMP phosphodiesterase hydrolyzes both pyrimidine and purine cyclic 2':3'- and 3':5'-nucleotides. , 1982, The Journal of biological chemistry.

[18]  G. Krishna,et al.  Cytidylate cyclase: possible artifacts in the methodology. , 1979, Science.

[19]  G. Krishna,et al.  Cytidylate cyclase: the product isolated by the method of Cech and Ignarro is not cytidine 3',5'-monophosphate. , 1979, Biochemical and biophysical research communications.

[20]  A. Bloch,et al.  Demonstration, in leukemia L-1210 cells, of a phosphodiesterase acting on 3':5'-cyclic CMP but not on 3':5'-cyclic AMP or 3':5'-cyclic GMP. , 1978, The Journal of biological chemistry.

[21]  P. Greengard.  Phosphorylated proteins as physiological effectors. , 1978, Science.

[22]  L. Ignarro,et al.  Cytidine 3',5'-monophosphate (cyclic CMP) formation by homogenates of mouse liver. , 1978, Biochemical and biophysical research communications.

[23]  L. Ignarro,et al.  Cytidine 3',5'-monophosphate (cyclic CMP) formation in mammalian tissues. , 1977, Science.

[24]  A. Bloch,et al.  Cytidine 3',5'-monophosphate (cyclic CMP). I. Isolation from extracts of leukemia L-1210 Cells. , 1974, Biochemical and biophysical research communications.

[25]  E. Sutherland,et al.  Adenyl Cyclase I. DISTRIBUTION, PREPARATION, AND PROPERTIES , 1962 .

[26]  E. Sutherland,et al.  Fractionation and characterization of a cyclic adenine ribonucleotide formed by tissue particles. , 1958, The Journal of biological chemistry.

[27]  M. Schiller,et al.  Immobilized Iron(III) Metal Affinity Chromatography for the Separation of Phosphorylated Macromolecules: Ligands and Applications , 1997 .

[28]  K. Kaibuchi,et al.  Small GTP-binding proteins. , 1992, International review of cytology.

[29]  M. Eckersley,et al.  The use ofm-nitrobenzyl alcohol as a matrix in fast atom bombardment mass spectrometry , 1988 .

[30]  R. Newton,et al.  Cyclic CMP phosphodiesterase: isolation, specificity and kinetic properties. , 1986, The International journal of biochemistry.

[31]  E. Sutherland,et al.  The relationship of epinephrine and glucagon to liver phosphorylase. IV. Effect of epinephrine and glucagon on the reactivation of phosphorylase in liver homogenates. , 1957, The Journal of biological chemistry.