Optimal design of a new structure piezo-driven cell injector

With the development of micromanipulation technique, the piezo-driven cell injector has been widely applied in cell microinjection. Traditional injection technology used piezo actuator to drive pipettes and a small mercury column was applied in micropipette to diminish the lateral tip oscillations. This injector significantly improves the survival rates of the ICSI process, but large lateral tip oscillations of the micropipette and the toxicity of mercury may damage to the cell membrane with a lower survival rate. Based on the theoretical basis of the previous generation injection structure and conventional injection system, a new design of the piezo-driven microinjector is proposed for microinjection. In this paper, the new structure uses packaged piezo actuator as driving source for connecting more stable and reliable. And it adopts two points flexible way to fix the pipette instead of the way of one point. The design parameters have been optimized for meeting the requirements of small cell injection. It's more simple and easy to use. This paper used simulation software to study the lateral vibration of the new structure, and optimized design size for minimum the lateral oscillation of micropipettes. Harmful lateral tip oscillations of micropipette are reduced substantially during simulation. Finally, we make the new microinjector by 3D printing technology for future experiment. This new structure will reduce the damage to cells in a large extent with a high success rate.

[1]  Yu Sun,et al.  Microfabricated glass devices for rapid single cell immobilization in mouse zygote microinjection , 2009, Biomedical microdevices.

[2]  Fumihito Arai,et al.  Microknife using ultrasonic vibration , 2000, MHS2000. Proceedings of 2000 International Symposium on Micromechatronics and Human Science (Cat. No.00TH8530).

[3]  Yu Xie,et al.  A force control based cell injection approach in a bio-robotics system , 2009, 2009 IEEE International Conference on Robotics and Automation.

[4]  Nejat Olgac,et al.  New technology for cellular piercing: rotationally oscillating μ-injector, description and validation tests , 2007, Biomedical microdevices.

[5]  Mingxuan Fan,et al.  Vibration study of the piezodriven pipettes immersed in viscous liquids , 2006 .

[6]  Dong Sun,et al.  A universal piezo-driven ultrasonic cell microinjection system , 2011, Biomedical microdevices.

[7]  Cong Lu.  A Cell Preparation Stage for Automatic Cell Injection , 2011 .

[8]  Hideki Yamamoto,et al.  A Visual Servo System for Microinjection under Stereoscopic Microscope , 2000 .

[9]  Dong Sun,et al.  Characterizing Mechanical Properties of Biological Cells by Microinjection , 2010, IEEE Transactions on NanoBioscience.

[10]  Yu Sun,et al.  Three-Dimensional Rotation of Mouse Embryos , 2012, IEEE Transactions on Biomedical Engineering.

[11]  Sunita Chauhan,et al.  Bio-effects and safety of low-intensity, low-frequency ultrasonic exposure. , 2012, Progress in biophysics and molecular biology.

[12]  Peter C. Y. Chen,et al.  A micromanipulation system with dynamic force-feedback for automatic batch microinjection , 2007 .

[13]  Fumihito Arai,et al.  Mechanical micro-dissection by microknife using ultrasonic vibration and ultra fine touch probe sensor , 2001, Proceedings 2001 ICRA. IEEE International Conference on Robotics and Automation (Cat. No.01CH37164).

[14]  Yu Sun,et al.  Orientation Control of Biological Cells Under Inverted Microscopy , 2011, IEEE/ASME Transactions on Mechatronics.

[15]  Mehmet Toner,et al.  Rotationally oscillating drill (Ros‐Drill©) for mouse icsi without using mercury , 2008, Molecular reproduction and development.

[16]  Nejat Olgaç,et al.  Microdynamics of the piezo-driven pipettes in ICSI , 2004, IEEE Transactions on Biomedical Engineering.

[17]  Sung-Yong Cho,et al.  A new micro biological cell injection system , 2004, 2004 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS) (IEEE Cat. No.04CH37566).

[18]  Mingzhu Sun,et al.  A novel pneumatic micropipette aspiration method using a balance pressure model. , 2013, The Review of scientific instruments.

[19]  Xinyu Liu,et al.  Automated mouse embryo injection moves toward practical use , 2009, 2009 IEEE International Conference on Robotics and Automation.

[20]  Bradley J. Nelson,et al.  Microrobotic cell injection , 2001, Proceedings 2001 ICRA. IEEE International Conference on Robotics and Automation (Cat. No.01CH37164).