Phosphate release and heavy metal accumulation by biofilm-immobilized and chemically-coupled cells of a Citrobacter sp. pre-grown in continuous culture.

A heavy metal-accumulating Citrobacter sp. was grown in carbon-limiting continuous culture in an air-lift fermentor containing raschig rings as support for biofilm development. Planktonic cells from the culture outflow were immobilized in parallel on raschig rings by chemical coupling (silanization), for quantitative comparison of phosphatase activity and uranyl uptake by both types of immobilized cell. The flow rate giving 50% conversion of substrate to product (phosphate) in flow-through reactors was higher, by 35-40%, for the biofilm-immobilized cells, possibly exploiting a pH-buffering effect of inorganic phosphate species within the extracellular polymeric material. Upon incorporation of uranyl ions (0.2 mM UO22+), both types of cell removed more than 90% of the input UO22+ at slow flow rates, but the chemically-coupled cells performed better at higher flow rates. The deposited material (HUO2PO4) subsequently removed Ni2+ from a second flow via intercalative ion exchange of Ni2+ into the crystalline HUO2PO4.4H2O lattice. This occurred irrespective of the method of coupling of the biomass to the support and suggested that uranyl phosphate accumulated by both types of cell has potential as a bio-inorganic ion exchanger-a potential use for the uranium recoved from primary waste treatment processes.

[1]  P. Poole,et al.  Purification and chacterization of acid-type phosphatases from a heavy-metal-accumulating Citrobacter sp. , 1998, Archives of Microbiology.

[2]  G. Grime,et al.  Identification of the Nickel Uranyl Phosphate Deposits on Citrobacter sp. Cells by Electron Microscopy with Electron Probe X-ray Microanalysis and by Proton-Induced X-ray Emission Analysis , 1998 .

[3]  L. Macaskie,et al.  Effect of substrate concentration and nitrate inhibition on product release and heavy metal removal by a Citrobacter sp. , 1997, Biotechnology and bioengineering.

[4]  B. Jeong,et al.  Localization of enzymically enhanced heavy metal accumulation by Citrobacter sp. and metal accumulation in vitro by liposomes containing entrapped enzyme. , 1997, Microbiology.

[5]  L. Macaskie,et al.  Microbially enhanced chemisorption of nickel into biologically synthesized hydrogen uranyl phosphate: a novel system for the removal and recovery of metals from aqueous solutions. , 1997, Biotechnology and bioengineering.

[6]  B. Jeong,et al.  Phosphotransferase activity of acid phosphatases of a Citrobacter sp. , 1997 .

[7]  M G Roig,et al.  Bioremediation of uranium-bearing wastewater: biochemical and chemical factors influencing bioprocess application. , 1997, Biotechnology and bioengineering.

[8]  L. Macaskie,et al.  Bioaccumulation of nickel by intercalation into polycrystalline hydrogen uranyl phosphate deposited via an enzymatic mechanism , 1996, Nature Biotechnology.

[9]  Paul Stoodley,et al.  Relation between the structure of an aerobic biofilm and transport phenomena , 1995 .

[10]  L. Macaskie,et al.  Enhancement of uranium bioaccumulation by a Citrobacter sp. via enzymically‐mediated growth of polycrystalline NH4UO2PO4 , 1995 .

[11]  R. Empson,et al.  Enzymatically‐mediated uranium accumulation and uranium recovery using a Citrobacter sp. Immobilised as a biofilm within a plug‐flow reactor , 1995 .

[12]  T. Beveridge,et al.  Microbial Biofilms: Mineralized Bacterial Biofilms in Sulphide Tailings and in Acid Mine Drainage Systems , 1995 .

[13]  J. Kennedy,et al.  Enzymically-enhanced extraction of uranium from biologically leached solutions , 1995 .

[14]  Hans-Curt Flemming,et al.  Sorption sites in biofilms , 1995 .

[15]  C. A. Kent,et al.  Biomass production for the removal of heavy metals from aqueous solutions at low pH using growth-decoupled cells of a Citrobacter sp. , 1995 .

[16]  M. Tsezos,et al.  A systematic study on equilibrium and kinetics of biosorptive accumulation. The case of Ag and Ni , 1995 .

[17]  J. Costerton,et al.  Microbial Biofilms: Growth of Microorganisms on Surfaces , 1995 .

[18]  B. Jeong,et al.  Enzymically accelerated biomineralization of heavy metals: application to the removal of americium and plutonium from aqueous flows. , 1994, FEMS microbiology reviews.

[19]  Z Lewandowski,et al.  Biofilms, the customized microniche , 1994, Journal of bacteriology.

[20]  J. Thomas-Oates,et al.  The structure of the O-specific polysaccharide of Citrobacter O16 containing glycerol phosphate. , 1994, European journal of biochemistry.

[21]  D. Kirchman,et al.  Attachment Stimulates Exopolysaccharide Synthesis by a Bacterium , 1993, Applied and environmental microbiology.

[22]  A. Chakrabarty,et al.  Exopolysaccharide production in biofilms: substratum activation of alginate gene expression by Pseudomonas aeruginosa , 1993, Applied and environmental microbiology.

[23]  C. Grey,et al.  Uranium bioaccumulation by a Citrobacter sp. as a result of enzymically mediated growth of polycrystalline HUO2PO4. , 1992, Science.

[24]  G. Gadd,et al.  Microbial Control of Pollution , 1992 .

[25]  L. Macaskie,et al.  Phosphatase production by a Citrobacter sp. growing in batch cultures retarded by anaerobic or osmotic stress, and the effect of the osmoprotectant glycine betaine. , 1991, FEMS microbiology letters.

[26]  L. Macaskie.  The application of biotechnology to the treatment of wastes produced from the nuclear fuel cycle: biodegradation and bioaccumulation as a means of treating radionuclide-containing streams. , 1991, Critical reviews in biotechnology.

[27]  L. Macaskie.  An immobilized cell bioprocess for the removal of heavy metals from aqueous flows. , 2007, Journal of chemical technology and biotechnology.

[28]  C. F. Forster,et al.  A comparative study of the nature of biopolymers extracted from anaerobic and activated sludges , 1990 .

[29]  L. Macaskie,et al.  Actinide and lanthanum toxicity towards aCitrobacter sp.: Uptake of lanthanum and a strategy for the biological treatment of liquid wastes containing plutonium , 1990, Bulletin of environmental contamination and toxicology.

[30]  S. Miller,et al.  A two-component regulatory system (phoP phoQ) controls Salmonella typhimurium virulence. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[31]  S. Kjelleberg,et al.  The role of an extracellular polysaccharide produced by the marine Pseudomonas sp. S9 in cellular detachment during starvation , 1989 .

[32]  L. Macaskie,et al.  Microbial metabolism, desolubilization, and deposition of heavy metals: metal uptake by immobilized cells and application to the detoxification of liquid wastes. , 1989, Advances in biotechnological processes.

[33]  A. Norberg,et al.  Development of a continuous process for metal accumulation by Zoogloea ramigera , 1984, Biotechnology and bioengineering.

[34]  S. Fesik,et al.  Structure and metal-binding properties of lipopolysaccharides from heptoseless mutants of Escherichia coli studied by 13C and 31P nuclear magnetic resonance. , 1983, The Journal of biological chemistry.

[35]  J W Wimpenny,et al.  Penetration of oxygen into bacterial colonies. , 1983, Journal of general microbiology.

[36]  S. Fesik,et al.  Characterization of lipopolysaccharide from a heptoseless mutant of Escherichia coli by carbon 13 nuclear magnetic resonance. , 1983, The Journal of biological chemistry.

[37]  C. Rolz,et al.  A new technology to ferment sugar cane directly the EX-FERM process. , 1980 .

[38]  W. Pierpoint.  The phosphatase and metaphosphatase activities of pea extracts. , 1957, The Biochemical journal.