Protein Microarrays: Overview, Applications and Challenges

Microarrays technology represents a new tool to address high-throughput biological studies. Various types of protein microarrays based on the application, format and content find use in basic research, drug and biomarker discovery, as well as for the characterization of proteins. In this chapter, we discuss advantages and limitations of protein microarrays, their features and recent applications. We also consider the different methods to build protein microarrays and the recent advances in cell free protein expression systems to construct in situ protein microarrays. Finally, we describe four types of self-assembled protein microarrays: PISA (protein array to protein Array); DAPA (DNA to Protein Array); PuCa (in situ puromycin array) and NAPPA (Nucleic Acids Programmable Protein Arrays) and the recent applications of this latter in situ protein array.

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