Chemical analysis of a polysaccharide of unripe (green) tomato (Lycopersicon esculentum).

A polysaccharide (PS-I) isolated from the aqueous extract of the unripe (green) tomatoes (Lycopersicon esculentum) consists of D-galactose, D-methyl galacturonate, D-arabinose, L-arabinose, and L-rhamnose. Structural investigation of the polysaccharide was carried out using total acid hydrolysis, methylation analysis, periodate oxidation study, and NMR studies ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments the structure of the repeating unit of the polysaccharide (PS-I) was established as: [structure: see text].

[1]  S. S. Islam,et al.  Structural investigation of a polysaccharide (Fr. I) isolated from the aqueous extract of an edible mushroom, Volvariella diplasia. , 2008, Carbohydrate research.

[2]  A. Gil-Serrano,et al.  Structural analysis of the exopolysaccharide produced by Pediococcus damnosus 2.6. , 1997, Carbohydrate research.

[3]  M. Rinaudo,et al.  13C NMR structural investigation of scleroglucan , 1982 .

[4]  Ionel Ciucanu,et al.  A simple and rapid method for the permethylation of carbohydrates , 1984 .

[5]  S. S. Islam,et al.  The structure and conformation of a water-insoluble (1-->3)-,(1-->6)-beta-d-glucan from the fruiting bodies of Pleurotus florida. , 2008, Carbohydrate research.

[6]  H. Wagner.  Search for plant derived natural products with immunostimulatory activity: recent advances , 1990 .

[7]  S. S. Islam,et al.  Structural investigation of a heteropolysaccharide isolated from the pods (fruits) of Moringa oleifera (Sajina). , 2007, Carbohydrate research.

[8]  M. Quinn,et al.  Botanical polysaccharides: macrophage immunomodulation and therapeutic potential. , 2006, International immunopharmacology.

[9]  D. Himmelsbach,et al.  13C-N.m.r. spectrum of a d-galactose-rich polysaccharide from tomato fruit , 1984 .

[10]  B. Stavric.  Role of chemopreventers in human diet. , 1994, Clinical biochemistry.

[11]  S. Wallner,et al.  Characteristics of tomato cell wall degradation in vitro: implications for the study of fruit-softening enzymes. , 1977, Plant physiology.

[12]  K. Gross.  Composition of ethanol-insoluble polysaccharides in water extracts of ripening tomatoes , 1986 .

[13]  S. S. Islam,et al.  Isolation and characterization of a heteroglycan from the fruits of Astraeus hygrometricus. , 2008, Carbohydrate research.

[14]  S. S. Islam,et al.  Structural studies of a methyl galacturonosyl-methoxyxylan isolated from the stem of Lagenaria siceraria (Lau). , 2008, Carbohydrate research.

[15]  Xiao-yu Li,et al.  Structural characterization and biological activities of SC4, an acidic polysaccharide from Salvia chinensis. , 2002, Acta pharmacologica Sinica.

[16]  Wang Shu-xiu,et al.  Immunoactivities of the polysaccharides from Morus alba, Chlamydomonas mexicana and Poria cocos , 1995 .

[17]  N. Maness,et al.  Determination of the degree of methyl esterification of pectins in small samples by selective reduction of esterified galacturonic acid to galactose. , 1990, Analytical biochemistry.

[18]  J. Vliegenthart,et al.  Determination of the d and l configuration of neutral monosaccharides by high-resolution capillary g.l.c. , 1978 .

[19]  E. Furusawa,et al.  An immunomodulatory polysaccharide‐rich substance from the fruit juice of Morinda citrifolia (noni) with antitumour activity , 1999, Phytotherapy research : PTR.

[20]  K. Hård,et al.  The Asn-linked carbohydrate chains of human Tamm-Horsfall glycoprotein of one male. Novel sulfated and novel N-acetylgalactosamine-containing N-linked carbohydrate chains. , 1992, European journal of biochemistry.

[21]  S. S. Islam,et al.  A water-soluble glucan isolated from an edible mushroom Termitomyces microcarpus. , 2007, Carbohydrate research.

[22]  S. Svensson,et al.  Determination of the Anomeric Configuration of Sugar Residues in Acetylated Oligo- and Polysaccharides by Oxidation with Chromium Trioxide in Acetic Acid. , 1972 .

[23]  S. S. Islam,et al.  Chemical analysis of a new fucoglucan isolated from an edible mushroom, Termitomyces robustus. , 2008, Carbohydrate research.

[24]  F. Smith,et al.  Use of Lithium Aluminium Hydride in the Study of Carbohydrates , 1950, Nature.

[25]  C. Hara,et al.  Anti-inflammatory activity and conformational behavior of a branched (1→3)-β-d-glucan from an alkaline extract of dictyophora indusiata fisch , 1982 .

[26]  G. Seymour,et al.  Composition and structural features of cell wall polysaccharides from tomato fruits , 1990 .

[27]  P. Agrawal,et al.  NMR spectroscopy in the structural elucidation of oligosaccharides and glycosides. , 1992, Phytochemistry.

[28]  S. Kacew,et al.  In vitro chemopreventive effects of plant polysaccharides (Aloe barbadensis miller, Lentinus edodes, Ganoderma lucidum and Coriolus versicolor). , 1999, Carcinogenesis.

[29]  S. S. Islam,et al.  Structural investigation of a heteroglycan isolated from the fruit bodies of an ectomycorrhizal fungus Astraeus hygrometricus. , 2007, Carbohydrate research.

[30]  S. S. Islam,et al.  Structural analysis of a water-soluble glucan (Fr.I) of an edible mushroom, Pleurotus sajor-caju. , 2007, Carbohydrate research.

[31]  S. S. Islam,et al.  Structural analysis of a polysaccharide isolated from the aqueous extract of an edible mushroom, Pleurotus sajor-caju, cultivar Black Japan. , 2008, Carbohydrate research.