EXPERIMENTAL STUDIES OF PLEIOTROPY AND EPISTASIS IN ESCHERICHIA COLI. I. VARIATION IN COMPETITIVE FITNESS AMONG MUTANTS RESISTANT TO VIRUS T4

Mutants selected for novel phenotypes frequently exhibit maladaptive pleiotropic effects. One may reasonably ask whether these effects are properties of the novel phenotypes per se, or whether these effects depend upon the particular genotypes conferring the novel phenotypes. To address this issue, I examined an array of independent mutants, derived from Escherichia coli B, that were all completely resistant to the virus T4. Each resistant mutant had maladaptive pleiotropic effects, but there was highly significant variation in competitive fitness among mutants. The degree of reduction in competitive fitness was strongly associated with cross‐resistance to virus T7 and with the inferred position of the mutated gene in a complex metabolic pathway. This variation in competitive fitness permits refinement of the resistant phenotype by selection among resistant genotypes. This mechanism complements refinement of the resistant phenotype by selection for epistatic modifiers of maladaptive pleiotropic effects.

[1]  R. Lenski.  EXPERIMENTAL STUDIES OF PLEIOTROPY AND EPISTASIS IN ESCHERICHIA COLI. II. COMPENSATION FOR MALADAPTIVE EFFECTS ASSOCIATED WITH RESISTANCE TO VIRUS T4 , 1988, Evolution; international journal of organic evolution.

[2]  D. Futuyma,et al.  GENETIC VARIATION AND COVARIATION IN RESPONSES TO HOST PLANTS BY ALSOPHILA POMETARIA (LEPIDOPTERA: GEOMETRIDAE) , 1987, Evolution; international journal of organic evolution.

[3]  A. Hoffmann,et al.  Genetic divergence under uniform selection. II. Different responses to selection for knockdown resistance to ethanol among Drosophila melanogaster populations and their replicate lines. , 1986, Genetics.

[4]  Joe C. Campbell,et al.  Developmental Constraints and Evolution: A Perspective from the Mountain Lake Conference on Development and Evolution , 1985, The Quarterly Review of Biology.

[5]  R. Lenski,et al.  Constraints on the Coevolution of Bacteria and Virulent Phage: A Model, Some Experiments, and Predictions for Natural Communities , 1985, The American Naturalist.

[6]  W. B. Watt.  Bioenergetics and Evolutionary Genetics: Opportunities for New Synthesis , 1985, The American Naturalist.

[7]  L. Luckinbill.  An Experimental Analysis of a Life History Theory , 1984 .

[8]  S. Via.  THE QUANTITATIVE GENETICS OF POLYPHAGY IN AN INSECT HERBIVORE. II. GENETIC CORRELATIONS IN LARVAL PERFORMANCE WITHIN AND AMONG HOST PLANTS , 1984, Evolution; international journal of organic evolution.

[9]  R. Lenski.  Two-step resistance by Escherichia coli B to bacteriophage T2. , 1984, Genetics.

[10]  B. Bachmann.  Linkage map of Escherichia coli K-12, edition 7 , 1983, Microbiological reviews.

[11]  D. Hartl,et al.  Selection in chemostats. , 1983, Microbiological reviews.

[12]  R. Lenski,et al.  APHID GENOTYPES, PLANT PHENOTYPES, AND GENETIC DIVERSITY: A DEMOGRAPHIC ANALYSIS OF EXPERIMENTAL DATA , 1982, Evolution; international journal of organic evolution.

[13]  J. A. Mckenzie,et al.  The effect of genetic background on the fitness of diazinon resistance genotypes of the Australian sheep blowfly, Lucilia cuprina , 1982, Heredity.

[14]  D. Dykhuizen,et al.  An Experimental Model: Bacterial Specialists and Generalists Competing in Chemostats , 1980 .

[15]  F. M. Stewart,et al.  Resource-Limited Growth, Competition, and Predation: A Model and Experimental Studies with Bacteria and Bacteriophage , 1977, The American Naturalist.

[16]  G. Schmidt,et al.  On a bacteriophage T3 and T4 receptor region within the cell wall lipopolysaccharide of Escherichia coli B. , 1976, Journal of molecular biology.

[17]  P. Prehm,et al.  Cell-wall lipopolysaccharide from Escherichia coli B. , 1975, European Journal of Biochemistry.

[18]  G. Ames,et al.  Protein Composition of the Outer Membrane of Salmonella typhimurium: Effect of Lipopolysaccharide Mutations , 1974, Journal of bacteriology.

[19]  S. Tamaki,et al.  Increase in Sensitivity to Antibiotics and Lysozyme on Deletion of Lipopolysaccharides in Escherichia coli Strains , 1973, Journal of Bacteriology.

[20]  T. Sato,et al.  Role of Lipopolysaccharides in Antibiotic Resistance and Bacteriophage Adsorption of Escherichia coli K-12 , 1971, Journal of bacteriology.

[21]  W. Wood,et al.  Interaction of bacteriophage T4 tail fiber components with a lipopolysaccharide fraction from Escherichia coli. , 1970, Journal of molecular biology.

[22]  H. Bungay,et al.  Capsular protection against virulent coliphage infection , 1970, Biotechnology and bioengineering.

[23]  D. E. Bradley,et al.  Ultrastructure of bacteriophage and bacteriocins , 1967, Bacteriological reviews.

[24]  E. Caspari.  PLEIOTROPIC GENE ACTION , 1952 .

[25]  U. Fano,et al.  Bacteriophage-Resistant Mutants in Escherichia Coli. , 1945, Genetics.

[26]  R. Punnett,et al.  The Genetical Theory of Natural Selection , 1930, Nature.

[27]  R. Lenski.  Dynamics of Interactions between Bacteria and Virulent Bacteriophage , 1988 .

[28]  G. M. Clarke,et al.  Developmental stability of insecticide resistant phenotypes in blowfly; a result of canalizing natural selection , 1987, Nature.

[29]  Nova Scotia.  ANTAGONISTIC PLEIOTROPY, DOMINANCE, AND GENETIC VARIATION* , 1982 .

[30]  A. Wright,et al.  Lipopolysaccharide as a Bacteriophage Receptor , 1980 .

[31]  W. Weidel.  Bacterial viruses; with particular reference to adsorption/penetration. , 1958, Annual review of microbiology.

[32]  D. E. Bradley,et al.  Ultrastructure of Bacteriophages and Bacteriocins OF BACTERIOPHAGE NUCLEIC ACIDS , 2022 .