Effects of r-Lactamases and omp Mutation on Susceptibility to 3-Lactam Antibiotics in Escherichia coli

Four types of P-lactamases consisting of a penicillinase type I (TEM-1), a penicillinase type II (OXA-1), a cephalosporinase of Citrobacter freundii, and a cephalosporinase of Proteus vulgaris were introduced into Escherichia coli MC4100 and its omp mutants, MH1160 (MC4100 ompRI) and MH760 (MC4100 ompR2), by transformation. Effects of the combination of the omp mutations and these P-lactamases on the susceptibility of E. coli strains were studied with 15 ,B-lactam antibiotics including cephalosporins, cephamycins, penicillins, imipenem, and aztreonam. The ompRI mutant, MH1160, lacks OmpF and OmpC, and it showed reduced susceptibility to 11 of the 15 P-lactam agents. The reduction in susceptibility to cefoxitin, moxalactam, and flomoxef was much greater than reduction in susceptibility to the other agents. When the ompRI mutant produced the cephalosporinase of C. freundii, the susceptibility of the mutant to 12 of the 15 P-lactam antibiotics decreased. The reduction in susceptibility of MH1160 to 10 of the 12 agents affected by the enzyme was twoto fourfold greater than that observed in MC4100. Such a synergistic effect was also observed with the cephalosporinase of P. vulgaris and ompRI mutation against six cephalosporins, moxalactam, and aztreonam.

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