Amperometric Biosensor Based on Diamine Oxidase/Platinum Nanoparticles/Graphene/Chitosan Modified Screen-Printed Carbon Electrode for Histamine Detection

This work describes the development and optimization studies of a novel biosensor employed in the detection and quantification of histamine in freshwater fish samples. The proposed biosensor is based on a modified carbon screen-printed electrode with diamineoxidase, graphene and platinum nanoparticles, which detects the hydrogen peroxide formed by the chemical process biocatalysed by the enzyme diamine oxidase and immobilized onto the nanostructurated surface of the receptor element. The amperometric measurements with the biosensor have been implemented in buffer solution of pH 7.4, applying an optimal low potential of +0.4 V. The novel biosensor shows high sensitivity (0.0631 μA·μM), low detection limit (2.54 × 10−8 M) and a broad linear domain from 0.1 to 300 μM. The applicability in natural complex samples and the analytical parameters of this enzyme sensor have been performed in the quantification of histamine in freshwater fish. An excellent correlation among results achieved with the developed biosensor and results found with the standard method for all freshwater fish samples has been achieved.

[1]  L. De Santis,et al.  Determination of biogenic amines in fish tissues by ion-exchange chromatography with conductivity detection. , 2004, Journal of chromatography. A.

[2]  John H. T. Luong,et al.  Amperometric biosensor for diamine using diamine oxidase purified from porcine kidney , 1997 .

[3]  Anthony P F Turner,et al.  Biosensors: sense and sensibility. , 2013, Chemical Society reviews.

[4]  J. M. Landete,et al.  Improved enzymatic method for the rapid determination of histamine in wine , 2004, Food additives and contaminants.

[5]  G. Vinci,et al.  Fast determination of biogenic amines in beverages by a core-shell particle column. , 2015, Food chemistry.

[6]  M. Es‐Souni,et al.  A versatile approach to processing of high active area pillar coral- and sponge-like Pt-nanostructures. Application to electrocatalysis , 2011 .

[7]  Jun Liu,et al.  Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film. , 2009, Talanta.

[8]  A. Salleh,et al.  An amperometric biosensor for the rapid assessment of histamine level in tiger prawn (Penaeus monodon) spoilage , 2007 .

[9]  A. Boffi,et al.  Characterization and application of a diamine oxidase from Lathyrus sativus as component of an electrochemical biosensor for the determination of biogenic amines in wine and beer , 2011, Analytical and bioanalytical chemistry.

[10]  A. Herr,et al.  Protein immobilization techniques for microfluidic assays. , 2013, Biomicrofluidics.

[11]  A. Shalaby.  Significance of biogenic amines to food safety and human health , 1996 .

[12]  J. Bartrolí,et al.  Amperometric biosensor for the determination of histamine in fish samples. , 2013, Food chemistry.

[13]  Sheng-nan Wu,et al.  Hydrogen peroxide sensing using ultrathin platinum-coated gold nanoparticles with core@shell structure. , 2013, Biosensors & bioelectronics.

[14]  V. Zucolotto,et al.  Biomimetic biosensor based on lipidic layers containing tyrosinase and lutetium bisphthalocyanine for the detection of antioxidants. , 2011, Biosensors & bioelectronics.

[15]  I. Shin,et al.  Improved HPLC Method Using 2,3-naphthalenedicarboxaldehyde as Fluorescent Labeling Agent for Quantification of Histamine in Human Immunoglobulin Preparations , 2011, Osong public health and research perspectives.

[16]  Qiang Zhao,et al.  High Sensitive and Selective Sensing of Hydrogen Peroxide Released from Pheochromocytoma Cells Based on Pt-Au Bimetallic Nanoparticles Electrodeposited on Reduced Graphene Sheets , 2015, Sensors.

[17]  I. Apetrei,et al.  The biocomposite screen-printed biosensor based on immobilization of tyrosinase onto the carboxyl functionalised carbon nanotube for assaying tyramine in fish products , 2015 .

[18]  P. Lieberman.  The basics of histamine biology. , 2011, Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology.

[19]  Yuyan Shao,et al.  Graphene Based Electrochemical Sensors and Biosensors: A Review , 2010 .

[20]  M. Gershwin,et al.  Histamine (Scombroid) Fish Poisoning: a Comprehensive Review , 2016, Clinical Reviews in Allergy & Immunology.

[21]  Yang Song,et al.  Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials. , 2016, Biosensors & bioelectronics.

[22]  M. Šebela,et al.  Spoilage Detection with Biogenic Amine Biosensors, Comparison of Different Enzyme Electrodes , 2012 .

[23]  H. Katano,et al.  Evaluation of an Electrochemical Method for the Analysis of Enzymatic Inhibition Reactions , 2013, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[24]  J. Bond,et al.  Fluorometric determination of histamine in tuna: development of method. , 1977, Journal - Association of Official Analytical Chemists.

[25]  K. Novoselov,et al.  A roadmap for graphene , 2012, Nature.

[26]  Á. Corbí,et al.  Biogenic amines in fermented foods , 2010, European Journal of Clinical Nutrition.

[27]  A. L. Hart,et al.  Electrochemical oxidation of hydrogen peroxide at platinum electrodes. Part 1. An adsorption-controlled mechanism , 1998 .

[28]  Felippe J. Pavinatto,et al.  Optimized architecture for Tyrosinase-containing Langmuir–Blodgett films to detect pyrogallol , 2011 .

[29]  T. K. Christopoulos,et al.  High-throughput chemiluminometric genotyping of single nucleotide polymorphisms of histamine, serotonin, and adrenergic receptor genes. , 2009, Analytical biochemistry.

[30]  M. El-Deab,et al.  Flower-shaped gold nanoparticles: Preparation, characterization, and electrocatalytic application , 2017 .

[31]  I. Apetrei,et al.  Amperometric biosensor based on polypyrrole and tyrosinase for the detection of tyramine in food samples , 2013 .

[32]  K. Kivirand,et al.  Biosensors for Biogenic Amines: The Present State of Art Mini-Review , 2011 .

[33]  Constantin Apetrei,et al.  Study of Different Carbonaceous Materials as Modifiers of Screen-Printed Electrodes for Detection of Catecholamines , 2015, IEEE Sensors Journal.

[34]  S. Bose,et al.  Recent advances in graphene-based biosensors. , 2011, Biosensors & bioelectronics.

[35]  H. Deeth,et al.  Biogenic Amines in Fish: Roles in Intoxication, Spoilage, and Nitrosamine Formation—A Review , 2009, Critical reviews in food science and nutrition.

[36]  Olga Domínguez-Renedo,et al.  Disposable biosensors for determination of biogenic amines. , 2010, Analytica chimica acta.

[37]  J. Thalhamer,et al.  Quantification of histamine in blood plasma and cell culture supernatants: a validated one-step gas chromatography-mass spectrometry method. , 2002, Analytical biochemistry.

[38]  B. Ye,et al.  A novel, colorimetric method for biogenic amine detection based on arylalkylamine N-acetyltransferase. , 2015, Chemical communications.

[39]  T. Nakano,et al.  A simple and rapid method for histamine analysis in fish and fishery products by TLC determination , 2011 .

[40]  J. Rohrer,et al.  Determination of biogenic amines in alcoholic beverages by ion chromatography with suppressed conductivity detection and integrated pulsed amperometric detection. , 2007, Journal of chromatography. A.

[41]  Ronald H. Schmidt,et al.  Food Safety Handbook , 2003 .

[42]  Tai Hyun Park,et al.  Real-time monitoring of geosmin and 2-methylisoborneol, representative odor compounds in water pollution using bioelectronic nose with human-like performance. , 2015, Biosensors & bioelectronics.