Protochlorophyllide Reductase III: Synthesis of a Protochlorophyllide‐Dihydroflavin Complex

Abstract— A mild and efficient method of linking a dihydroflavin to the C‐17 carboxylic acid side chain of protochlorophyllide, without degradation of the sensitive E ring or loss of magnesium, is described. The appended dihydroflavin was shown to quench the fluorescence of protochlorophyllide. In contrast, a dihydronicotinamide moiety was unable to effect fluorescence quenching. The relevance of these findings to a possible mechanism of action of the enzyme protochlorophyllide reductase is discussed.

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