Naphthalene degradation by Pseudomonas sp. HOB1: in vitro studies and assessment of naphthalene degradation efficiency in simulated microcosms.

Naphthalene, being a ubiquitous pollutant of the environment and a perilous material, its biodegradation has been receiving constant scientific consideration. Highly potential, naphthalene degrading bacteria were isolated from sediments of polluted Amlakadi canal, Gujarat, India. Among the isolates, Pseudomonas sp. HOB1, showed ability to degrade 2000 ppm naphthalene within 24h. The culture exhibited potential to tolerate as high as 60,000 ppm of naphthalene. Statistical approach was used to analyze the effect of physiological parameters and initial biomass concentration on naphthalene degradation. Naphthalene degradation was found to be augmented in the pH range of 7.5-8.5. Naphthalene degradation was maximum in the temperature range of 35-37 degrees C and initial inoculum size of more than 1.8 ml of 1.0 A(660). Simulated microcosm studies in the presence and absence of indigenous microflora confirmed its ability for naphthalene degradation and to colonize the soil. Pseudomonas sp. HOB1 was found to be highly potent in degrading higher concentrations of naphthalene under laboratory conditions as well as in simulated microcosms.

[1]  Hauke Harms,et al.  Principles of microbial PAH-degradation in soil. , 2005, Environmental pollution.

[2]  H. Harms,et al.  Mass transfer limitation of microbial growth and pollutant degradation , 1997, Journal of Industrial Microbiology and Biotechnology.

[3]  O. Singh,et al.  Polycyclic aromatic hydrocarbons: environmental pollution and bioremediation. , 2002, Trends in biotechnology.

[4]  I. Kosheleva,et al.  Efficiency of naphthalene biodegradation by Pseudomonas putida G7 in soil , 2004 .

[5]  L. Brown,et al.  Effect of Nitrogen Source on End Products of Naphthalene Degradation , 1981, Applied and environmental microbiology.

[6]  S. Harayama,et al.  Molecular Characterization and Substrate Preference of a Polycyclic Aromatic Hydrocarbon Dioxygenase from Cycloclasticus sp. Strain A5 , 2003, Applied and Environmental Microbiology.

[7]  U. Trivedi,et al.  Application of response surface methodology for optimization of lactic acid production using date juice , 2006 .

[8]  C. Peters,et al.  Multisubstrate biodegradation kinetics of naphthalene, phenanthrene, and pyrene mixtures. , 1999, Biotechnology and bioengineering.

[9]  J. Costerton,et al.  Microbial Biofilms: Introduction to Microbial Biofilms , 1995 .

[10]  K. Pagilla,et al.  Enhanced electrokinetic dissolution of naphthalene and 2,4-DNT from contaminated soils. , 2006, Journal of hazardous materials.

[11]  X. Xia,et al.  DAMBE: software package for data analysis in molecular biology and evolution. , 2001, The Journal of heredity.

[12]  W. Frankenberger,et al.  Influence of surfactants on pyrene desorption and degradation in soils , 1996, Applied and environmental microbiology.

[13]  S. Grimberg,et al.  Quantifying the biodegradation of phenanthrene by Pseudomonas stutzeri P16 in the presence of a nonionic surfactant , 1996, Applied and environmental microbiology.

[14]  Jinwon Park,et al.  Effect of additional carbon source on naphthalene biodegradation by Pseudomonas putida G7. , 2003, Journal of hazardous materials.

[15]  J. N. Baruah,et al.  Predominant role of hydrocarbon solubilization in the microbial uptake of hydrocarbons , 1982, Biotechnology and bioengineering.

[16]  M. Nei,et al.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. , 2007, Molecular biology and evolution.

[17]  W. Rulkens,et al.  Influence of nonionic surfactants on bioavailability and biodegradation of polycyclic aromatic hydrocarbons , 1995, Applied and environmental microbiology.

[18]  B. Mahjoub,et al.  Effects of aging on the extractability of naphthalene and phenanthrene from Mediterranean soils. , 2007, Journal of hazardous materials.

[19]  J. Grotenhuis,et al.  Influence of hydrodynamic conditions on naphthalene dissolution and subsequent biodegradation. , 1998, Biotechnology and bioengineering.

[20]  Ann C. Grimm,et al.  Chemotaxis of Pseudomonas spp. to the polyaromatic hydrocarbon naphthalene , 1997, Applied and environmental microbiology.

[21]  Woojun Park,et al.  Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. , 2004, International journal of systematic and evolutionary microbiology.

[22]  Yanli Sun,et al.  Effects of mixed surfactants on the volatilization of naphthalene from aqueous solutions. , 2007, Journal of hazardous materials.

[23]  C. Cerniglia,et al.  Naphthalene biodegradation in environmental microcosms: estimates of degradation rates and characterization of metabolites , 1987, Applied and environmental microbiology.

[24]  A. Anderson,et al.  Isolation and Characterization of Polycyclic Aromatic Hydrocarbon–Degrading Mycobacterium Isolates from Soil , 2004, Microbial Ecology.

[25]  J. N. Petersen,et al.  Assessment of naphthalene biodegradation efficiency of Pseudomonas and Burkholderia strains tested in soil model systems , 2006 .

[26]  A. Tiehm.  Degradation of polycyclic aromatic hydrocarbons in the presence of synthetic surfactants , 1994, Applied and environmental microbiology.

[27]  A. Mrozik,et al.  Bacterial degradation and bioremediation of polycyclic aromatic hydrocarbons , 2003 .

[28]  J. A. Veen,et al.  Habitable pore space and survival ofRhizobium leguminosarum biovartrifolii introduced into soil , 1990, Microbial Ecology.

[29]  S. Strand,et al.  Anaerobic biodegradation of naphthalene, phenanthrene, and biphenyl by a denitrifying enrichment culture. , 2001, Water research.

[30]  D. Madamwar,et al.  Response surface methodology for optimization of medium for decolorization of textile dye Direct Black 22 by a novel bacterial consortium. , 2008, Bioresource technology.

[31]  B. Chang,et al.  Biodegradation of polycyclic aromatic hydrocarbons by a mixed culture. , 2000, Chemosphere.