Molecular cloning of the interleukin-1 beta converting enzyme.

Interleukin-1 beta (IL-1 beta) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor. A complementary DNA encoding a protease that carries out this cleavage has been cloned. Recombinant expression in COS-7 cells enabled the cells to process precursor IL-1 beta to the mature form. Sequence analysis indicated that the enzyme itself may undergo proteolytic processing. The gene encoding the protease was mapped to chromosomal band 11q23, a site frequently involved in rearrangement in human cancers.

[1]  S. Dower,et al.  The interleukin-1 system: receptors, ligands and signals. , 1992, Chemical immunology.

[2]  S. Dower,et al.  The Interleukin-1 System: Receptors, Ligands and Signals , 1992 .

[3]  T. Barbui,et al.  Modulation of cell proliferation and cytokine production in acute myeloblastic leukemia by interleukin-1 receptor antagonist and lack of its expression by leukemic cells. , 1991, Blood.

[4]  R. Thompson,et al.  Blocking IL-1: interleukin 1 receptor antagonist in vivo and in vitro. , 1991, Immunology today (Amsterdam. Regular ed.).

[5]  J. Weidner,et al.  IL-1-converting enzyme requires aspartic acid residues for processing of the IL-1 beta precursor at two distinct sites and does not cleave 31-kDa IL-1 alpha. , 1991, Journal of immunology.

[6]  K. Huebner,et al.  A novel IL‐1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types. , 1991, The EMBO journal.

[7]  Z. Estrov,et al.  Suppression of chronic myelogenous leukemia colony growth by interleukin-1 (IL-1) receptor antagonist and soluble IL-1 receptors: a novel application for inhibitors of IL-1 activity , 1991 .

[8]  C. Dinarello.  Interleukin-1 and interleukin-1 antagonism. , 1991, Blood.

[9]  T. Barbui,et al.  Interleukin‐1 and interleukin‐2 control granulocyte‐ and granulocyte‐macrophage colony‐stimulating factor gene expression and cell proliferation in cultured acute myeloblastic leukemia , 1990, International journal of cancer.

[10]  R. Black,et al.  Substrate specificity of the protease that processes human interleukin-1 beta. , 1990, The Journal of biological chemistry.

[11]  S. Dower,et al.  Regulation of alloreactivity in vivo by a soluble form of the interleukin-1 receptor. , 1990, Science.

[12]  D. Hazuda,et al.  Processing of precursor interleukin 1 beta and inflammatory disease. , 1990, The Journal of biological chemistry.

[13]  F. Hecht,et al.  Chromosome localization of human ARH genes, a ras-related gene family. , 1990, Genomics.

[14]  G. Duff,et al.  Interleukin 1: the first interleukin. , 1990, Immunology today.

[15]  Alan I. Alpert,et al.  The murine interleukin-4 receptor: Molecular cloning and characterization of secreted and membrane bound forms , 1989, Cell.

[16]  K. Kaushansky,et al.  Interleukin-1 stimulates proliferation of acute myeloblastic leukemia cells by induction of granulocyte-macrophage colony-stimulating factor release. , 1989, Blood.

[17]  P. Cameron,et al.  Identification of a monocyte specific pre-interleukin 1 beta convertase activity. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[18]  R. Black,et al.  Activation of interleukin‐ 1β by a co‐induced protease , 1989 .

[19]  F. Cozzolino,et al.  Interleukin 1 as an autocrine growth factor for acute myeloid leukemia cells. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[20]  L. Lanier,et al.  Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain. , 1989, Science.

[21]  M. Frohman,et al.  Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[22]  C. March,et al.  cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily. , 1988, Science.

[23]  R. Black,et al.  Generation of biologically active interleukin-1 beta by proteolytic cleavage of the inactive precursor. , 1988, The Journal of biological chemistry.

[24]  C. March,et al.  Stimulation of B-cell progenitors by cloned murine interleukin-7 , 1988, Nature.

[25]  M. Matsuoka,et al.  Autocrine stimulation of interleukin 1 beta in acute myelogenous leukemia cells , 1987, The Journal of experimental medicine.

[26]  M. Kozak.  An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. , 1987, Nucleic acids research.

[27]  P. Matsudaira,et al.  Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. , 1987, The Journal of biological chemistry.

[28]  A. C. Webb,et al.  Studies on the molecular nature of human interleukin 1. , 1987, Journal of immunology.

[29]  D. Cerretti,et al.  Cloning, sequence, and expression of bovine interleukin 2. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[30]  C. March,et al.  Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs , 1985, Nature.

[31]  A. C. Webb,et al.  Nucleotide sequence of human monocyte interleukin 1 precursor cDNA. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[32]  J. Devereux,et al.  A comprehensive set of sequence analysis programs for the VAX , 1984, Nucleic Acids Res..

[33]  C. March,et al.  Cloning, sequence and expression of human interleukin-2 receptor , 1984, Nature.

[34]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.