Resistance of Escherichia coli to Penicillins IX. Genetics and Physiology of Class II Ampicillin-Resistant Mutants That Are Galactose Negative or Sensitive to Bacteriophage C21, or Both

Ampicillin-resistant mutants of class II are determined by a doubling of chromosomally and episomally mediated ampicillin resistance on agar plates. Several mutants were isolated from a female as well as from an Hfr strain. The mutants differed from each other in various properties such as response to colicin E2 and sodium cholate, response to the phages T4 and C21, and fermentation of galactose. By conjugation and transduction experiments, it was shown that mutations in at least four loci gave the class II phenotype. The mutations were found to be in the galU gene, the ctr gene, and two new genes close to mtl denoted lpsA and lpsB. The carbohydrate compositions of the lipopolysaccharides of the mutants were investigated and found to be changed compared to the parent strains. GalU mutants lacked rhamnose and galactose and had 11% glucose compared to the parent strain. The lpsA mutant also lacked rhamnose and had only traces of galactose and 58% glucose, whereas the lpsB mutant contained 14% rhamnose, traces of galactose, and 81% glucose compared to the parent strain.

[1]  H. G. Boman,et al.  Ampicillin-Resistant Mutants of Escherichia coli K-12 with Lipopolysaccharide Alterations Affecting Mating Ability and Susceptibility to Sex-Specific Bacteriophages , 1971, Journal of bacteriology.

[2]  K. Nordström,et al.  Colicin Tolerance Induced by Ampicillin or Mutation to Ampicillin Resistance in a Strain of Escherichia coli K-12 , 1971, Journal of bacteriology.

[3]  T. Sato,et al.  Role of Lipopolysaccharides in Antibiotic Resistance and Bacteriophage Adsorption of Escherichia coli K-12 , 1971, Journal of bacteriology.

[4]  O. Lüderitz.  Recent results on the biochemistry of the cell wall lipopolysaccharides of Salmonella bacteria. , 1970, Angewandte Chemie.

[5]  S. Normark,et al.  Genetics of a chain-forming mutant of Escherichia coli. Transduction and dominance of the envA gene mediating increased penetration to some antibacterial agents. , 1970, Genetical research.

[6]  David A. Monner,et al.  Female strains of Escherichia coli K12 as selective hosts for the isolation of female specific mutants of phage omega II. , 1970, Biochemical and biophysical research communications.

[7]  K. Sanderson.  Current linkage map of Salmonella typhimurium. , 1970, Bacteriological reviews.

[8]  K. Nordström,et al.  Resistance of Escherichia coli to Penicillins VIII. Physiology of a Class II Ampicillin-Resistant Mutant , 1970, Journal of bacteriology.

[9]  H. G. Boman,et al.  Resistance of Escherichia coli to Penicillins VI. Purification and Characterization of the Chromosomally Mediated Penicillinase Present in ampA-Containing Strains , 1970, Journal of bacteriology.

[10]  P. D. de Haan,et al.  Recombination in Escherichia coli. 3. Mapping by the gradient of transmission. , 1969, Mutation research.

[11]  Frank F. Richards,et al.  The Genetics of Bacteria and their Viruses , 1969, The Yale Journal of Biology and Medicine.

[12]  R. J. Wang,et al.  Carbohydrate Accumulation and Metabolism in Escherichia coli: the Close Linkage and Chromosomal Location of ctr Mutations , 1969, Journal of bacteriology.

[13]  K. Nordström,et al.  Resistance of Escherichia coli to penicillins: III. AmpB, a locus affecting episomally and chromosomally mediated resistance to ampicillin and chloramphenicol , 1968 .

[14]  K. G. ERIKSSON-GRENNBERG,et al.  Resistance of Escherichia coli to penicillins. II. An improved mapping of the ampA gene. , 1968, Genetical research.

[15]  S. Normark,et al.  Resistance of Escherichia coli to penicillins. IV. Genetic study of mutants resistant to D,L-ampicillin concentrations o 100 mu-g-ml. , 1968, Genetical research.

[16]  T. Holme,et al.  CHEMICAL COMPOSITION OF CELL-WALL POLYSACCHARIDE OF ROUGH MUTANTS OF SALMONELLA TYPHIMURIUM. , 1968 .

[17]  L. Leive.  Studies on the permeability change produced in coliform bacteria by ethylenediaminetetraacetate. , 1968, The Journal of biological chemistry.

[18]  B. Low.  Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. , 1968, Proceedings of the National Academy of Sciences of the United States of America.

[19]  R. J. Wang,et al.  Carbohydrate accumulation and metabolism in Escherichia coli. I. Description of pleiotropic mutants. , 1968, Journal of molecular biology.

[20]  A. L. Taylor,et al.  Revised linkage map of Escherichia coli. , 1967, Bacteriological reviews.

[21]  A. L. Taylor,et al.  Revised linkage map of Escherichia coli , 1967 .

[22]  S. Luria,et al.  Genetics and Physiology of Colicin-tolerant Mutants of Escherichia coli , 1967, Journal of bacteriology.

[23]  M. Nomura,et al.  Interaction of Colicins with Bacterial Cells III. Colicin-tolerant Mutations in Escherichia coli , 1967, Journal of bacteriology.

[24]  D. Fraenkel,et al.  Glucose and Gluconate Metabolism in an Escherichia coli Mutant Lacking Phosphoglucose Isomerase , 1967, Journal of bacteriology.

[25]  H. Kalckar,et al.  INACTIVATION OF PHAGE C21 BY VARIOUS PREPARATIONS FROM LIPOPOLYSACCHARIDE OF E. COLI K-12* , 1966, Proceedings of the National Academy of Sciences of the United States of America.

[26]  J. Shapiro.  Chromosomal location of the gene determining uridine diphoglucose formation in Escherichia coli K-12 , 1966, Journal of bacteriology.

[27]  Gratia Jp.  Studies on defective lysogeny due to chromosomal deletion in Escherichia coli. I. Single lysogens. , 1966 .

[28]  H. Mayer,et al.  COMPLEX POLYSACCHARIDES IN DIFFERENT STRAINS OF ESCHERICHIA COLI K‐12 * , 1966, Annals of the New York Academy of Sciences.

[29]  N. Datta,et al.  The relation of resistance transfer factors to the F-factor (sex-factor) of Escherichia coli K12. , 1966, Genetical research.

[30]  L. Leive,et al.  Release of lipopolysaccharide by EDTA treatment of E. coli. , 1965, Biochemical and biophysical research communications.

[31]  H. G. Boman,et al.  Resistance of Escherichia coli to Penicillins I. Genetic Study of Some Ampicillin-Resistant Mutants , 1965, Journal of bacteriology.

[32]  L. Rothfield,et al.  Lipopolysaccharide of the Gram-Negative Cell Wall , 1964, Science.

[33]  S. Brenner,et al.  A CHEMICAL BASIS FOR THE HOST-INDUCED MODIFICATION OF T-EVEN BACTERIOPHAGES. , 1963, Proceedings of the National Academy of Sciences of the United States of America.

[34]  H. Kalckar,et al.  Biochemical observations on E. coli mutants defective in uridine diphosphoglucose. , 1962, Proceedings of the National Academy of Sciences of the United States of America.

[35]  G. Stent,et al.  A genetic locus for the regulation of ribonucleic acid synthesis. , 1961, Proceedings of the National Academy of Sciences of the United States of America.

[36]  T. Fukasawa,et al.  Galactose-sensitive mutants of Salmonella. II. Bacteriolysis induced by galactose. , 1961, Biochimica et biophysica acta.

[37]  E. Adelberg,et al.  Linkage Analysis with Very High Frequency Males of Escherichia Coli. , 1960, Genetics.

[38]  G. Bertani,et al.  STUDIES ON LYSOGENESIS I , 1951, Journal of bacteriology.

[39]  G. Schmidt,et al.  Immunochemistry of R lipopolysaccharides of Escherichia coli. Studies on R mutants with an incomplete core, derived from E. coli O8:K27. , 1970, European journal of biochemistry.

[40]  L. Pearce,et al.  Specific chromosomal affinity of a resistant factor. , 1968, Journal of general microbiology.

[41]  J. Gratia.  Studies on defective lysogeny due to chromosomal deletion in Escherichia coli. I. Single lysogens. , 1966, Biken journal.

[42]  B. Horecker.  The biosynthesis of bacterial polysaccharides. , 1966, Annual review of microbiology.

[43]  O. Westphal.  Bacterial lipopolysaccharides : extraction with phenol-water and further applications of the procedure , 1965 .

[44]  K. Kurahashi,et al.  MUTATIONS IN ESCHERICHIA COLI THAT AFFECT URIDINE DIPHOSPHATE GLUCOSE PYROPHOSPHORYLASE ACTIVITY AND GALACTOSE FERMENTATION. , 1963, Biochimica et biophysica acta.

[45]  J. Strominger,et al.  [143A] Determination of UDPG and UTP by means of UDPG dehydrogenase , 1957 .

[46]  H. Vogel,et al.  Acetylornithinase of Escherichia coli: partial purification and some properties. , 1956, The Journal of biological chemistry.