P-FAB: A Fiber-Optic Biosensor Device for Rapid Detection of COVID-19

Rapid and low-cost diagnosis of COVID-19 is essential to identify the infected subjects, particularly the asymptomatic cases, primarily to arrest the spread of the disease through local transmission. Antibody-based chromatographic serological tests, as an alternative to RT-PCR, offer only limited help due to high false positives. We propose to exploit our field-deployable/portable plasmonic fiber-optic absorbance biosensor (P-FAB) platform for one-step, wash-free detection of SARS-CoV-2 virus particles directly in saliva sample with minimal sample pre-processing.

[1]  Banshi D. Gupta,et al.  Fibre-optic evanescent field absorption sensor based on a U-shaped probe , 1996 .

[2]  Sunil K. Khijwania,et al.  Fiber optic evanescent field absorption sensor: Effect of fiber parameters and geometry of the probe , 1999 .

[3]  Banshi D. Gupta,et al.  Theoretical modelling of a bi-dimensional U-shaped surface plasmon resonance based fibre optic sensor for sensitivity enhancement , 2008 .

[4]  S. Mukherji,et al.  Novel U-bent fiber optic probe for localized surface plasmon resonance based biosensor. , 2009, Biosensors & bioelectronics.

[5]  S. Mukherji,et al.  A dendrimer matrix for performance enhancement of evanescent wave absorption-based fiber-optic biosensors , 2014 .

[6]  Bandaru Ramakrishna,et al.  Evanescent wave absorbance based U-bent fiber probe for immunobiosensor with gold nanoparticle labels , 2016 .

[7]  V. Sai,et al.  Development of LSPR based U-bent plastic optical fiber sensors , 2016 .

[8]  V. Sai,et al.  A wash-free, dip-type fiber optic plasmonic (DiP) assay for sub-zeptomole analyte detection. , 2018, 1810.06437.

[9]  V. Sai,et al.  Fiber Optic Plasmonic Sandwich Immunosensor: Influence of AuNP Label Size and Concentration , 2018, 2018 IEEE SENSORS.

[10]  P. Kalita,et al.  Plasmonic biosensors for bacterial endotoxin detection on biomimetic C-18 supported fiber optic probes. , 2019, Biosensors & bioelectronics.

[11]  Deborah A. Williamson,et al.  Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2 , 2020, Journal of Clinical Microbiology.

[12]  V. Sai,et al.  Investigating the Refractive Index Sensitivity of U-Bent Fiber Optic Sensors Using Ray Optics , 2019, Journal of Lightwave Technology.

[13]  Angelo Tagliabue,et al.  Saliva is a reliable tool to detect SARS-CoV-2 , 2020, Journal of Infection.

[14]  Matthew Osborne,et al.  Diagnosing COVID-19: The Disease and Tools for Detection , 2020, ACS nano.

[15]  O. Tsang,et al.  Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study , 2020, The Lancet Infectious Diseases.

[16]  Jonathan E. Schmitz,et al.  Laboratory Diagnosis of COVID-19: Current Issues and Challenges , 2020, Journal of Clinical Microbiology.

[17]  Shangen Zheng,et al.  Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2 , 2020, Journal of Clinical Microbiology.

[18]  S. Zheng,et al.  Evaluation of Nucleocapsid and Spike Protein-based ELISAs for detecting antibodies against SARS-CoV-2 , 2020, medRxiv.

[19]  Eric Song,et al.  Saliva is more sensitive for SARS-CoV-2 detection in COVID-19 patients than nasopharyngeal swabs , 2020, medRxiv.

[20]  Yan Yan,et al.  Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites , 2020, Acta Pharmaceutica Sinica B.

[21]  K. To,et al.  SARS‐CoV‐2: What can saliva tell us? , 2020, Oral diseases.