Comparative sequence analysis of duck and human hepatitis B virus genomes

We have cloned and sequenced an infectious, functionally active genome of a duck hepatitis B virus (DHBV). It is 3,021 base pairs (bp) in length and shows little DNA sequence homology to the genome of human hepatitis B virus (HBV). However, the amino acid sequences of predicted viral gene products are similar between DHBV and HBV, and the genome organization present in DHBV reflects that of HBV. As in the mammalian virus the long minus strand of the DHBV genome encodes three long overlapping reading frames designated as P, S, and C. The fourth open reading frame, termed X, is absent in DHBV. A comparison with a sequence of a second DHBV isolate [Mandart et al, Journal of Virology 49:782–792, 1984] revealed a nucleotide sequence variation of 5.6% and confirmed the presented overall gene organization of DHBV.

[1]  H. Will,et al.  Infectious hepatitis B virus from cloned DNA of known nucleotide sequence. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[2]  R. Sprengel,et al.  Cloned duck hepatitis B virus DNA is infectious in Pekin ducks , 1984, Journal of virology.

[3]  H. Varmus,et al.  Nucleotide sequence of an infectious molecularly cloned genome of ground squirrel hepatitis virus , 1984, Journal of virology.

[4]  J. Summers,et al.  Mapping of the cohesive overlap of duck hepatitis B virus DNA and of the site of initiation of reverse transcription , 1984, Journal of virology.

[5]  H. Will,et al.  Expression of hepatitis B antigens with a simian virus 40 vector , 1984, Journal of virology.

[6]  F. Galibert,et al.  Nucleotide sequence of a cloned duck hepatitis B virus genome: comparison with woodchuck and human hepatitis B virus sequences , 1984, Journal of virology.

[7]  P. Marion,et al.  Major polypeptide of duck hepatitis B surface antigen particles , 1983, Journal of virology.

[8]  H. Will,et al.  Signals regulating hepatitis B surface antigen transcription , 1983, Nature.

[9]  H. Delius,et al.  Structure of the hepatitis B virus genome , 1983, Journal of virology.

[10]  J. Kreft,et al.  Cloning and expression in Escherichia coli and Bacillus subtilis of the hemolysin (cereolysin) determinant from Bacillus cereus , 1983, Journal of bacteriology.

[11]  H. Varmus.  RNA viruses: Reverse transcription in plants? , 1983, Nature.

[12]  Asao Fujiyama,et al.  Cloning and structural analyses of hepatitis B virus DNAs, subtype adr , 1983, Nucleic Acids Res..

[13]  R. Lerner,et al.  Chemically synthesized peptides of hepatitis B surface antigen duplicate the d/y specificities and induce subtype-specific antibodies in chimpanzees. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[14]  Y. Ono,et al.  Tbe complete nudeotide sequences of the cloned hepatitis B vims DNA; subtype adr and adw , 1983 .

[15]  W. London,et al.  Naturally occurring infection of Pekin duck embryos by duck hepatitis B virus. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[16]  J. Taylor,et al.  Asymmetric replication of duck hepatitis B virus DNA in liver cells: Free minus-strand DNA. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[17]  J. Summers,et al.  Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediate , 1982, Cell.

[18]  S. Stahl,et al.  Hepatitis B virus core antigen: synthesis in Escherichia coli and application in diagnosis. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[19]  Gordon R. Dreesman,et al.  Antibody to hepatitis B surface antigen after a single inoculation of uncoupled synthetic HBsAg peptides , 1982, Nature.

[20]  Karl-Heinz Glatting,et al.  Computer programs for the analysis and the management of DNA sequences , 1982, Nucleic Acids Res..

[21]  F. Galibert,et al.  Nucleotide sequence of a cloned woodchuck hepatitis virus genome: comparison with the hepatitis B virus sequence , 1982, Journal of virology.

[22]  W. Ansorge,et al.  Improvements of DNA sequencing gels. , 1981, Analytical biochemistry.

[23]  R. Sprengel,et al.  Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium. , 1981, Nucleic acids research.

[24]  J. Summers.  Three recently described animal virus models for human hepatitis B virus , 1981, Hepatology.

[25]  A. Zuckerman.  Woodchuck, squirrel and duck hepatitis viruses , 1981, Nature.

[26]  Walter Gilbert,et al.  Hepatitis B virus genes and their expression in E. coli , 1979, Nature.

[27]  F. Galibert,et al.  Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli , 1979, Nature.

[28]  C. Richardson.  Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[29]  T. Miyata,et al.  Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus , 1983, Nature.

[30]  H. Will,et al.  Hepatitis B after infection of a chimpanzee with cloned HBV DNA. , 1983, Developments in biological standardization.

[31]  Rolf Sprengel.  Das Beta-Lactamase-Gen aus Bacillus licheniformis , 1983 .

[32]  The complete nucleotide sequences of the cloned hepatitis B virus DNA; subtype adr and adw. , 1983, Nucleic acids research.

[33]  A M Prince,et al.  Hepatitis B virus vaccine: identification of HBsAg/a and HBsAg/d but not HBsAg/y subtype antigenic determinants on a synthetic immunogenic peptide. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[34]  H. Will,et al.  Structure and function of the hepatitis B virus genome. , 1982, Princess Takamatsu symposia.

[35]  高松宮妃癌研究基金,et al.  Primary and tertiary structure of nucleic acids and cancer research. Proceedings of the 12th International Symposium of the Princess Takamatsu Cancer Research Fund, Tokyo, 1981. , 1982, Princess Takamatsu symposia.

[36]  A. Zuckerman,et al.  Viral hepatitis: report on a who informal consultation , 1981, Journal of medical virology.

[37]  W. Rutter,et al.  THE NUCLEOTIDE SEQUENCE OF THE HEPATITIS B VIRAL GENOME AND THE IDENTIFICATION OF THE MAJOR VIRAL GENES , 1980 .

[38]  W. Gilbert,et al.  Sequencing end-labeled DNA with base-specific chemical cleavages. , 1980, Methods in enzymology.