In vitro anti-tick properties of the essential oil of Tagetes minuta L. (Asteraceae) on Hyalomma rufipes (Acari: Ixodidae).

In this study we examined the anti-tick properties of the essential oil of Tagetes minuta L. (Asteraceae: Asterales) against Hyalomma rufipes ticks. We obtained the essential oil of T. minuta by hydro-distillation of a combination of fresh flowers, leaves and soft stems, and analysed these by using gas chromatography (GC) and gas chromatography-linked mass spectrometry (GC-MS). The oil had a high percentage of monoterpenes and the major compounds identified were cis-ocimene (28.5%), beta-ocimene (16.83%) and 3-methyl-2-(2-methyl-2-butenyl)-furan (11.94%). Hyalomma rufipes adults displayed a significant (P < 0.05) dose repellent response to the essential oil of T. minuta. Probit analysis indicated a repellent EC50 of T. minuta essential oil for male ticks to be 0.072 mL/mL (CI 0.053 mL/mL to 0.086 mL/mL) and 0.070 mL/mL (CI 0.052 mL/mL to 0.084 mL/mL) for female ticks. There were no significant differences in repellent responses between male and female ticks. The oil also significantly (P < 0.05) delayed moulting of 60% of H. rufipes engorged nymphs. These results suggest that T. minuta may be a potential source of anti-tick agents.

[1]  W. Evans.  Trease and Evans , 2013 .

[2]  S. Moore,et al.  Plant-based insect repellents: a review of their efficacy, development and testing , 2011, Malaria Journal.

[3]  P. Masika,et al.  An in-vivo study of the efficacy and safety of ethno-veterinary remedies used to control cattle ticks by rural farmers in the Eastern Cape Province of South Africa , 2009, Tropical Animal Health and Production.

[4]  W. Wanzala.  Ethnobotanicals for management of the brown ear tick Rhipicephalus appendiculatus in western Kenya , 2009 .

[5]  P. Masika,et al.  Tick control methods used by resource-limited farmers and the effect of ticks on cattle in rural areas of the Eastern Cape Province, South Africa , 2009, Tropical Animal Health and Production.

[6]  F. Kivaria.  Estimated direct economic costs associated with tick-borne diseases on cattle in Tanzania , 2006, Tropical Animal Health and Production.

[7]  A. Tripathi,et al.  Potential toxicity of new genotypes of Tagetes (Asteraceae) species against stored grain insect pests , 2005 .

[8]  R. Piccaglia,et al.  Characterization and Yield Evaluation of Essential Oils from Different Tagetes Species , 2004 .

[9]  S. Chown,et al.  Feeding patterns of immature stages of Hyalomma truncatum and Hyalomma marginatum rufipes on different hosts , 2000, Experimental & Applied Acarology.

[10]  A. Hassanali,et al.  Repellent and acaricidal properties of Ocimum suave against Rhipicephalus appendiculatus ticks , 1995, Experimental & Applied Acarology.

[11]  T. Kröber,et al.  In vitro assays for repellents and deterrents for ticks: differing effects of products when tested with attractant or arrestment stimuli , 2003, Medical and veterinary entomology.

[12]  Nilanjana Mukherjee,et al.  Organochlorine pesticide residues in sediments of a tropical mangrove estuary, India: implications for monitoring. , 2003, Environment international.

[13]  J. George,et al.  Resistance to Coumaphos and Diazinon in Boophilus microplus (Acari: Ixodidae) and Evidence for the Involvement of an Oxidative Detoxification Mechanism , 2003, Journal of medical entomology.

[14]  M. Debboun,et al.  Development of an In Vitro Method for the Evaluation of Candidate Repellents Against Leptotrombidium (Acari: Trombiculidae) Chiggers , 2003, Journal of medical entomology.

[15]  A. Estrada-Peña,et al.  Ticks of domestic animals in Africa: a guide to identification of species , 2003 .

[16]  J. F. Day,et al.  Comparative efficacy of insect repellents against mosquito bites. , 2002, The New England journal of medicine.

[17]  S Abdel-Shafy,et al.  In vitro acaricidal effect of plant extract of neem seed oil (Azadirachta indica) on egg, immature, and adult stages of Hyalomma anatolicum excavatum (Ixodoidea: Ixodidae). , 2002, Veterinary parasitology.

[18]  ohn,et al.  COMPARATIVE EFFICACY OF INSECT REPELLENTS AGAINST MOSQUITO BITES , 2002 .

[19]  Ø. Hammer,et al.  PAST: PALEONTOLOGICAL STATISTICAL SOFTWARE PACKAGE FOR EDUCATION AND DATA ANALYSIS , 2001 .

[20]  Albert J. Ndakala,et al.  Gynandropsis gynandra essential oil and its constituents as tick (Rhipicephalus appendiculatus) repellents , 1999 .

[21]  I. Jantan.  DEVELOPMENT OF ENVIRONMENT-FRIENDLY INSECT REPELLENTS FROM THE LEAF OILS OF SELECTED MALAYSIAN PLANTS , 1999 .

[22]  C. Madge,et al.  Therapeutic landscapes of the Jola, The Gambia, West Africa. , 1998, Health & place.

[23]  C. Lans,et al.  Ethnoveterinary medicines used for ruminants in Trinidad and Tobago. , 1998, Preventive veterinary medicine.

[24]  P. Houghton,et al.  Laboratory Handbook for the Fractionation of Natural Extracts , 1998, Springer US.

[25]  J. Carroll.  Kairomonal activity of white-tailed deer metatarsal gland substances: a more sensitive behavioral bioassay using Ixodes scapularis (Acari: Ixodidae). , 1998, Journal of medical entomology.

[26]  G. Kaaya,et al.  Evaluation of neem products for the control of the African ticks Rhipicephalus appendiculatus, Amblyomma variegatum, and Boophilus decoloratus , 1995 .

[27]  D. Sonenshine.  Biology of ticks. Volume I. , 1991 .

[28]  D. Sonenshine.  Biology of ticks , 1991 .

[29]  R. Pollack,et al.  Studies of the growth-regulating effects of ivermectin on larval Onchocerca lienalis in vitro. , 1987, The Journal of parasitology.

[30]  A. D. de Vos,et al.  Studies on a bovine Babesia transmitted by Hyalomma marginatum rufipes Koch, 1844. , 1981, The Onderstepoort journal of veterinary research.

[31]  P. Mccosker.  GLOBAL ASPECTS OF THE MANAGEMENT AND CONTROL OF TICKS OF VETERINARY IMPORTANCE , 1979 .