Application of ZnO/graphene and S6 aptamers for sensitive photoelectrochemical detection of SK-BR-3 breast cancer cells based on a disposable indium tin oxide device.

ZnO/graphene (ZnO/G) composite and S6 aptamer were employed to sensitive photoelectrochemical (PEC) strategy for the specific detection of SK-BR-3 cancer cells based on a portable indium tin oxide microdevice. ZnO/G composite was synthesized using a facile ultrasonic method, and then applied to improve the PEC performance due to the unique hollow structure of ZnO naospheres and the superior properties of graphene. Subsequently, S6 aptamer was applied to this specific detection of SK-BR-3 cancer cells. And the concentration of SK-BR-3 cells was measured with a low detection limit of 58 cells mL(-1) and a wide linear range of 1×10(2)-1×10(6) cells mL(-1), through the decrease in photocurrent intensity resulting from the increase in steric hindrances when specifically recognized with S6 aptamers. Excellent discrimination against target and analogous cells was demonstrated, indicating the high selectivity of the proposed cell sensor. Our work also demonstrated a sensitive, stable and low cytotoxicity approach for early and accurate detection of cancer cells.

[1]  Erwin Reisner,et al.  Photoelectrochemical water oxidation with photosystem II integrated in a mesoporous indium-tin oxide electrode. , 2012, Journal of the American Chemical Society.

[2]  Jutaek Nam,et al.  pH-Induced aggregation of gold nanoparticles for photothermal cancer therapy. , 2009, Journal of the American Chemical Society.

[3]  M. Mahmoudi,et al.  Graphene: promises, facts, opportunities, and challenges in nanomedicine. , 2013, Chemical reviews.

[4]  A. Gopalan,et al.  Glucose sensing, photocatalytic and antibacterial properties of graphene–ZnO nanoparticle hybrids , 2012 .

[5]  H. Emamy,et al.  Nontoxic concentrations of PEGylated graphene nanoribbons for selective cancer cell imaging and photothermal therapy , 2012 .

[6]  Huaqiang Cao,et al.  ZnO@graphene composite with enhanced performance for the removal of dye from water , 2011 .

[7]  Tailiang Guo,et al.  Improving the field emission of graphene by depositing zinc oxide nanorods on its surface , 2012 .

[8]  Ying Wang,et al.  Preparation, Structure, and Electrochemical Properties of Reduced Graphene Sheet Films , 2009 .

[9]  Z. Marković,et al.  In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes. , 2011, Biomaterials.

[10]  I-Wei Chen,et al.  Quantum‐Dot‐Tagged Reduced Graphene Oxide Nanocomposites for Bright Fluorescence Bioimaging and Photothermal Therapy Monitored In Situ , 2012, Advanced materials.

[11]  Kai Yang,et al.  The influence of surface chemistry and size of nanoscale graphene oxide on photothermal therapy of cancer using ultra-low laser power. , 2012, Biomaterials.

[12]  Jonathan P. Metters,et al.  New directions in screen printed electroanalytical sensors: an overview of recent developments. , 2011, The Analyst.

[13]  H. Dai,et al.  Ultrasmall reduced graphene oxide with high near-infrared absorbance for photothermal therapy. , 2011, Journal of the American Chemical Society.

[14]  Abdullah M. Asiri,et al.  One-step preparation of ZnO nanoparticle -decorated reduced graphene oxide composites and their application to photocurrent generation , 2012 .

[15]  Wei-Wei Zhao,et al.  The coupling of localized surface plasmon resonance-based photoelectrochemistry and nanoparticle size effect: towards novel plasmonic photoelectrochemical biosensing. , 2012, Chemical communications.

[16]  O. Akhavan.  Photocatalytic reduction of graphene oxides hybridized by ZnO nanoparticles in ethanol , 2011 .

[17]  Zhongai Hu,et al.  Zinc Oxide/Reduced Graphene Oxide Composites and Electrochemical Capacitance Enhanced by Homogeneous Incorporation of Reduced Graphene Oxide Sheets in Zinc Oxide Matrix , 2011 .

[18]  Qingming Shen,et al.  ZnO/CdS Hierarchical Nanospheres for Photoelectrochemical Sensing of Cu2+ , 2011 .

[19]  Yong-Min Huh,et al.  Isolation of RNA Aptamers Targeting HER -2-overexpressing Breast Cancer Cells Using Cell-SELEX , 2009 .

[20]  Zhouyi Guo,et al.  Synergistic effect of chemo-photothermal therapy using PEGylated graphene oxide. , 2011, Biomaterials.

[21]  Hong-Yan Chen,et al.  Reduced Graphene Oxide-Hierarchical ZnO Hollow Sphere Composites with Enhanced Photocurrent and Photocatalytic Activity , 2012 .

[22]  Jin Zhai,et al.  Two-dimensional graphene bridges enhanced photoinduced charge transport in dye-sensitized solar cells. , 2010, ACS nano.

[23]  Xianzhi Fu,et al.  TiO2-graphene nanocomposites for gas-phase photocatalytic degradation of volatile aromatic pollutant: is TiO2-graphene truly different from other TiO2-carbon composite materials? , 2010, ACS nano.

[24]  Danzhen Li,et al.  ZnO photonic crystals with enhanced photocatalytic activity and photostability , 2013 .

[25]  Guozhong Cao,et al.  Aggregation of ZnO nanocrystallites for high conversion efficiency in dye-sensitized solar cells. , 2008, Angewandte Chemie.

[26]  Zhenyu Lin,et al.  Fabrication of an electrically heated indium-tin-oxide electrode for electrochemiluminescent detection system. , 2009, The Analyst.

[27]  Kai Yang,et al.  Multimodal Imaging Guided Photothermal Therapy using Functionalized Graphene Nanosheets Anchored with Magnetic Nanoparticles , 2012, Advanced materials.

[28]  Yongfa Zhu,et al.  Photocorrosion Suppression of ZnO Nanoparticles via Hybridization with Graphite-like Carbon and Enhanced Photocatalytic Activity , 2009 .

[29]  C. N. Lau,et al.  Temperature dependence of the Raman spectra of graphene and graphene multilayers. , 2007, Nano letters.

[30]  Andre K. Geim,et al.  Raman spectrum of graphene and graphene layers. , 2006, Physical review letters.

[31]  I. Rendina,et al.  Self-assembled biofilm of hydrophobins protects the silicon surface in the KOH wet etch process. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[32]  Guozhong Cao,et al.  Hierarchically Structured ZnO Film for Dye‐Sensitized Solar Cells with Enhanced Energy Conversion Efficiency , 2007 .

[33]  M. Estévez,et al.  Using aptamer-conjugated fluorescence resonance energy transfer nanoparticles for multiplexed cancer cell monitoring. , 2009, Analytical chemistry.

[34]  Yueming Li,et al.  P25-graphene composite as a high performance photocatalyst. , 2010, ACS nano.

[35]  Haesik Yang,et al.  "Outer-sphere to inner-sphere" redox cycling for ultrasensitive immunosensors. , 2012, Analytical chemistry.

[36]  R. Guerrieri,et al.  Impedance measurement technique for high-sensitivity cell detection in microstructures with non-uniform conductivity distribution. , 2012, Lab on a chip.

[37]  Ruud H. Brakenhoff,et al.  Detection, clinical relevance and specific biological properties of disseminating tumour cells , 2008, Nature Reviews Cancer.

[38]  Omid Akhavan,et al.  Size-dependent genotoxicity of graphene nanoplatelets in human stem cells. , 2012, Biomaterials.

[39]  Kwang S. Kim,et al.  Large-scale pattern growth of graphene films for stretchable transparent electrodes , 2009, Nature.

[40]  J. S. Lee,et al.  Carbon-doped ZnO nanostructures synthesized using vitamin C for visible light photocatalysis , 2010 .

[41]  Kai Yang,et al.  Graphene in mice: ultrahigh in vivo tumor uptake and efficient photothermal therapy. , 2010, Nano letters.

[42]  R. Car,et al.  Raman spectra of graphite oxide and functionalized graphene sheets. , 2008, Nano letters.

[43]  Zhuo. Sun,et al.  Microwave-assisted synthesis of ZnO–graphene composite for photocatalytic reduction of Cr(VI) , 2011 .

[44]  S. Krishnamurthy,et al.  Detection of minimal residual disease in blood and bone marrow in early stage breast cancer , 2010, Cancer.

[45]  O. Akhavan,et al.  Toward single-DNA electrochemical biosensing by graphene nanowalls. , 2012, ACS nano.

[46]  Omid Akhavan,et al.  Graphene nanomesh promises extremely efficient in vivo photothermal therapy. , 2013, Small.

[47]  O. Akhavan.  Graphene nanomesh by ZnO nanorod photocatalysts. , 2010, ACS nano.