Motility-, autocorrelation-, and polarization-sensitive optical coherence tomography discriminates cells and gold nanorods within 3D tissue cultures.

We propose a method for differentiating classes of light scatterers based upon their temporal and polarization properties computed from time series of polarization-sensitive optical coherence tomography (PS-OCT) images. The amplitude (motility) and time scale (autocorrelation decay time) of the speckle fluctuations are combined with the cross-polarization pixel-wise to render Motility-, autocorrelation-, and polarization-sensitive (MAPS) OCT contrast images. This combination of metrics provides high specificity for discriminating diffusive gold nanorods and mammary epithelial cell spheroids within 3D tissue culture, based on their unique MAPS signature. This has implications toward highly specific contrast in molecular (nanoparticle-based) and functional (cellular activity) imaging using standard PS-OCT hardware.

[1]  Amy L. Oldenburg,et al.  Digital dispersion compensation for ultrabroad-bandwidth single-camera spectral-domain polarization-sensitive OCT , 2011, BiOS.

[2]  D. Boas,et al.  Dynamic light scattering optical coherence tomography. , 2012, Optics express.

[3]  C. Ayata,et al.  Quantitative Imaging of Cerebral Blood Flow Velocity and Intracellular Motility using Dynamic Light Scattering–Optical Coherence Tomography , 2013, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[4]  A. Oldenburg,et al.  Monitoring airway mucus flow and ciliary activity with optical coherence tomography , 2012, Biomedical optics express.

[5]  Ruikang K. Wang,et al.  Three-dimensional high-resolution imaging of gold nanorods uptake in sentinel lymph nodes. , 2011, Nano letters.

[6]  S. Rawal,et al.  Molecular histopathology using gold nanorods and optical coherence tomography. , 2013, Investigative ophthalmology & visual science.

[7]  A. Oldenburg,et al.  Imaging three-dimensional rotational diffusion of plasmon resonant gold nanorods using polarization-sensitive optical coherence tomography. , 2011, Physical review. E, Statistical, nonlinear, and soft matter physics.

[8]  T. V. van Leeuwen,et al.  Path-length-resolved diffusive particle dynamics in spectral-domain optical coherence tomography. , 2010, Physical review letters.

[9]  Mina J. Bissell,et al.  Putting tumours in context , 2001, Nature Reviews Cancer.

[10]  B. N. Khlebtsov,et al.  Depolarization of light scattered by gold nanospheres and nanorods , 2006 .

[11]  Adrian Mariampillai,et al.  Detecting apoptosis using dynamic light scattering with optical coherence tomography. , 2011, Journal of biomedical optics.

[12]  J. Fujimoto,et al.  Photothermal detection of gold nanoparticles using phase-sensitive optical coherence tomography. , 2008, Optics express.

[13]  Francisco E. Robles,et al.  Molecular imaging true-colour spectroscopic optical coherence tomography. , 2011, Nature photonics.

[14]  Samantha M. Miller,et al.  Role of HGF in epithelial–stromal cell interactions during progression from benign breast disease to ductal carcinoma in situ , 2013, Breast Cancer Research.

[15]  M. V. van Gemert,et al.  Two-dimensional birefringence imaging in biological tissue using polarization-sensitive optical coherence tomography , 1997, European Conference on Biomedical Optics.

[16]  Amy L. Oldenburg,et al.  Longitudinal Study of Mammary Epithelial and Fibroblast Co-Cultures Using Optical Coherence Tomography Reveals Morphological Hallmarks of Pre-Malignancy , 2012, PloS one.

[17]  W. Steen.  Absorption and Scattering of Light by Small Particles , 1999 .

[18]  Melissa C Skala,et al.  Photothermal optical coherence tomography of epidermal growth factor receptor in live cells using immunotargeted gold nanospheres. , 2008, Nano letters.

[19]  T. Fischer,et al.  Imaging and Elastometry of Blood Clots Using Magnetomotive Optical Coherence Tomography and Labeled Platelets , 2012, IEEE Journal of Selected Topics in Quantum Electronics.

[20]  Scott Barry,et al.  OCT methods for capillary velocimetry , 2012, Biomedical optics express.

[21]  David D. Nolte,et al.  Tissue dynamics spectroscopy for phenotypic profiling of drug effects in three-dimensional culture , 2012, Biomedical optics express.

[22]  B. Nikoobakht,et al.  種結晶を媒介とした成長法を用いた金ナノロッド(NR)の調製と成長メカニズム , 2003 .

[23]  Amy L Oldenburg,et al.  Imaging gold nanorods in excised human breast carcinoma by spectroscopic optical coherence tomography. , 2009, Journal of materials chemistry.

[24]  Travis A. Meyer,et al.  In vivo photothermal optical coherence tomography of gold nanorod contrast agents , 2012, Biomedical optics express.

[25]  Alaaldin M. Alkilany,et al.  Gold nanorods: their potential for photothermal therapeutics and drug delivery, tempered by the complexity of their biological interactions. , 2012, Advanced drug delivery reviews.

[26]  Mostafa A. El-Sayed,et al.  Preparation and Growth Mechanism of Gold Nanorods (NRs) Using Seed-Mediated Growth Method , 2003 .

[27]  Ryan L. Shelton,et al.  Fourier domain pump-probe optical coherence tomography imaging of Melanin , 2009, 2009 Conference on Lasers and Electro-Optics and 2009 Conference on Quantum electronics and Laser Science Conference.