Evidence for a Role of Post-Ovulatory Cumulus Components in Supporting Fertilizing Ability of Hamster Spermatozoa

A completely chemically defined culture medium was used to investigate the role of egg-cumulus complex (ECC) components in supporting sperm fertilizing ability. Defined sperm motility-stimulating factors (hypotaurine and epinephrine), with polyvinylalcohol as the macromolecular component, were included in the defined medium. Freshly-ovulated hamster ECCs were incubated with washed epididymal spermatozoa under different conditions designed to evaluate the ability of ECC components to support sperm capacitation, acrosome reactions, and the ability to penetrate the ova. The major conclusions from the data are that ECC components are capable of supporting these physiologic events, and that these components are present in the soluble (fluid) compartment of the ECC. This work is the first in a series of steps aimed at the localization, characterization, and eventual identification of the natural acrosome reaction-inducing stimulus associated with the ECC.

[1]  B. Bavister,et al.  Effects of epinephrine and hypotaurine on in-vitro fertilization in the golden hamster. , 1982, Journal of reproduction and fertility.

[2]  R. Yanagimachi,et al.  Sperm-egg ratios and the site of the acrosome reaction during in vivo fertilization in the hamster , 1982 .

[3]  B. Bavister.  Substitution of a synthetic polymer for protein in a mammalian gamete culture system. , 1981, The Journal of experimental zoology.

[4]  B. Bavister,et al.  The effects of taurine and hypotaurine on in vitro fertilization in the golden hamster , 1981 .

[5]  R. Yanagimachi.  Mechanisms of Fertilization in Mammals , 1981 .

[6]  B. Bavister.  RECENT PROGRESS IN THE STUDY OF EARLY EVENTS IN MAMMALIAN FERTILIZATION * , 1980, Development, growth & differentiation.

[7]  R. Mrsny,et al.  TAURINE AND HYPOTAURINE: THEIR EFFECTS ON MOTILITY, CAPACITATION AND THE ACROSOME REACTION OF HAMSTER SPERM IN VITRO AND THEIR PRESENCE IN SPERM AND REPRODUCTIVE TRACT FLUIDS OF SEVERAL MAMMALS * , 1980, Development, growth & differentiation.

[8]  H. Moore,et al.  An in vivo analysis of factors influencing the fertilization of hamster eggs. , 1978, Biology of reproduction.

[9]  B. Bavister,et al.  The effects of cauda epididymal plasma on the motility and acrosome reaction of hamster and guinea pig spermatozoa in vitro. , 1978, Biology of reproduction.

[10]  D. Wolf,et al.  Calcium-dependent binding of mouse epididymal spermatozoa to the zona pellucida. , 1978, Developmental biology.

[11]  B. Bavister,et al.  The effects of sperm extracts and energy sources on the motility and acrosome reaction of hamster spermatozoa in vitro. , 1977, Biology of reproduction.

[12]  P. Hoppe,et al.  An albumin requirement for fertilization of mouse eggs in vitro. , 1974, Journal of reproduction and fertility.

[13]  M. C. Chang,et al.  The importance of serum albumin and metabolic intermediates for capacitation of spermatozoa and fertilization of mouse eggs in vitro. , 1973, Journal of reproduction and fertility.

[14]  R. Gwatkin,et al.  Capacitation of hamster spermatozoa in vitro: the role of cumulus components. , 1972, Journal of reproduction and fertility.

[15]  Bedford Jm.  Sperm capacitation and fertilization in mammals. , 1970 .

[16]  R. Yanagimachi.  In vitro acrosome reaction and capacitation of golden hamster spermatozoa by bovine follicular fluid and its fractions , 1969 .

[17]  R. Yanagimachi.  In vitro capacitation of hamster spermatozoa by follicular fluid. , 1969, Journal of reproduction and fertility.

[18]  J. Bedford.  Ultrastructural changes in the sperm head during fertilization in the rabbit. , 1968, The American journal of anatomy.

[19]  C. Barros,et al.  In vitro fertilization and the sperm acrosome reaction in the hamster. , 1967, The Journal of experimental zoology.

[20]  C. R. Austin,et al.  Role of the rodent acrosome and perforatorium in fertilization , 1958, Proceedings of the Royal Society of London. Series B - Biological Sciences.