Cyclic Nucleotide Phosphodiesterase in Dictyostelium discoideum: Interconversion of Two Enzyme Forms

An extracellular cyclic nucleotide phosphodiesterase was isolated from either growing cultures or aggregating amoebas of Dictyostelium discoideum. The enzyme is released in a form with a low Michaelis constant (15 micromolar) and spontaneously undergoes a slow conversion to a less active form with a high Michaelis constant (2 mnillimolar). Inactivation was prevented or reversed by use of Cleland's reagent, dithiothreitol. The two enzyme forms may be part of a mechanism for control of concentration of cyclic adenosine monophosphate.

[1]  R. Pannbacker,et al.  Phosphodiesterase in Dictyostelium discoideum and the Chemotactic Response to Cyclic Adenosine Monophosphate , 1972, Science.

[2]  M. M. Appleman,et al.  Multiple cyclic nucleotide phosphodiesterase activities from rat brain. , 1971, Biochemistry.

[3]  J. Bonner,et al.  Evidence for a Second Chemotactic System in the Cellular Slime Mold, Dictyostelium discoideum , 1970, Journal of bacteriology.

[4]  B. Chassy,et al.  The acrasin activity of 3',5'-cyclic nucleotides. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[5]  B. Chassy,et al.  Acceleration of morphogenesis in Dictyostelium discoideum by exogenous mononucleotides. , 1969, Journal of general microbiology.

[6]  Philip C. Ritterbush,et al.  The Difference of Man and the Difference It Makes. Mortimer J. Adler. Holt, Rinehart and Winston, New York, 1967. xvi + 395 pp. $7.95. Based on the Encyclopaedia Britannica Lectures, Chicago, 1966 , 1968 .

[7]  J. Bonner,et al.  The acrasin activity of adenosine-3',5'-cyclic phosphate. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[8]  John Tyler Bonner,et al.  The Cellular Slime Molds , 1967 .

[9]  B. Davis.  DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS * , 1964, Annals of the New York Academy of Sciences.

[10]  B. Shaffer.  Aspects of Aggregation in Cellular Slime Moulds 1. Orientation and Chemotaxis , 1957, The American Naturalist.

[11]  B. Shaffer.  Properties of acrasin. , 1956, Science.

[12]  G. S. Eadie.  On the evaluation of the constants Vm and Km in enzyme reactions. , 1952, Science.

[13]  B. Hofstee.  On the evaluation of the constants Vm and KM in enzyme reactions. , 1952, Science.

[14]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[15]  J. Bonner.  Induction of stalk cell differentiation by cyclic AMP in the cellular slime mold Dictyostelium discoideum. , 1970, Proceedings of the National Academy of Sciences of the United States of America.