Complement Activation Alters Platelet Function

Abstract : We have established base line criteria that will allow us to evaluate the role of platelets during trauma. These studies have started to unravel the relationship between platelets and complement and their contribution to tissue damage. It seems that complement C3 does not deposit on the surface of platelets following ischemia/reperfusion. Yet, we have seen the deposition of both C3 and platelets in various tissues following IRI in a similar time frame. Further studies will evaluate if these factors co-localize in tissue. We have developed B6.lprPF4-/- mice which will allow us to better study the role of PF4 in tissue damage. Preliminary studies indicate a decreased level of platelets in these mice, suggesting a decreased level of disease. Further studies will expand upon these observations better outlining the function of platelets in the injury associated with trauma. Ultimately these studies will allow us to develop specific treatment strategies that limit battlefield tissue injury without affecting haemostatic and coagulation properties of platelets.