Doxorubicin-fucoidan-gold nanoparticles composite for dual-chemo-photothermal treatment on eye tumors

The current research demonstrates the feasible biomedical application of AuNPs coated with doxorubicin (Dox)-loaded fucoidan (Fu) for dual-chemotherapy and photothermal treatment (PTT) on eye tumors in vitro and in vivo. Marine-derived Fu was used as a capping agent to achieve high photostability for AuNPs, and Dox as a FDA-approved anti-cancer drug was added to induce chemotherapy. The synthesized Dox-Fu@AuNPs exhibited high cytotoxicity on the tumor cells and strong light absorption for temperature increase in vitro. After intratumoral injection of Dox-Fu@AuNPs in the rabbit eye tumors, PTT-assisted Dox-Fu@AuNPs entailed the complete removal of the eye tumors without recurrence for 14 days after the treatment. Photoacoustic image contrast from the tumor regions was enhanced due to selective light absorption by the administered Dox-Fu@AuNPs. Therefore, the proposed Dox-Fu@AuNPs can be a potential nano-theranostic material for treating and diagnosing the eye tumors.

[1]  T. Vo,et al.  Fucoidans as a natural bioactive ingredient for functional foods , 2013 .

[2]  H. Maeda,et al.  Mechanism of tumor-targeted delivery of macromolecular drugs, including the EPR effect in solid tumor and clinical overview of the prototype polymeric drug SMANCS. , 2001, Journal of controlled release : official journal of the Controlled Release Society.

[3]  Weiming Liu,et al.  In vitro cancer cell imaging and therapy using transferrin-conjugated gold nanoparticles. , 2009, Cancer letters.

[4]  Xiaolong Liang,et al.  Prussian blue coated gold nanoparticles for simultaneous photoacoustic/CT bimodal imaging and photothermal ablation of cancer. , 2014, Biomaterials.

[5]  C. Eng,et al.  Targeted therapy for uveal melanoma. , 2008, Cancer treatment reviews.

[6]  Eric P. Holowka,et al.  Drug Delivery: Materials Design and Clinical Perspective , 2014 .

[7]  I. Tannock,et al.  Drug penetration in solid tumours , 2006, Nature Reviews Cancer.

[8]  Ruth Duncan,et al.  Polymer conjugates as anticancer nanomedicines , 2006, Nature Reviews Cancer.

[9]  Kai Yang,et al.  Graphene in mice: ultrahigh in vivo tumor uptake and efficient photothermal therapy. , 2010, Nano letters.

[10]  P. Lam.  Minimal-access surgery for staging of malignant melanoma , 1996 .

[11]  Sungjee Kim,et al.  Gold nanoparticle-mediated photothermal therapy: current status and future perspective. , 2014, Nanomedicine.

[12]  Bo Li,et al.  Fucoidan: Structure and Bioactivity , 2008, Molecules.

[13]  Andrew D. Wong,et al.  Quantitative Analysis of the Enhanced Permeation and Retention (EPR) Effect , 2015, PloS one.

[14]  Wei Feng,et al.  Hollow silica nanoparticles loaded with hydrophobic phthalocyanine for near-infrared photodynamic and photothermal combination therapy. , 2013, Biomaterials.

[15]  Van Phuc Nguyen,et al.  Feasibility of photoacoustic evaluations on dual‐thermal treatment of ex vivo bladder tumors , 2017, Journal of biophotonics.

[16]  Lingrong Liu,et al.  Chitosan-coated gold nanorods for cancer therapy combining chemical and photothermal effects. , 2014, Macromolecular bioscience.

[17]  James C. Lin,et al.  Microwave Induced Temperature Rises in Rabbit Eyes in Cataract Research , 1975 .

[18]  J. Dickinson,et al.  Fucoidan and Cancer: A Multifunctional Molecule with Anti-Tumor Potential , 2015, Marine drugs.

[19]  Monya Baker,et al.  Nanotechnology imaging probes: smaller and more stable , 2010, Nature Methods.

[20]  Amit Jain,et al.  The intracellular drug delivery and anti tumor activity of doxorubicin loaded poly(gamma-benzyl L-glutamate)-b-hyaluronan polymersomes. , 2010, Biomaterials.

[21]  赵鹏飞,et al.  IR-780 dye loaded tumor targeting theranostic nanoparticles for NIR imaging and photothermal therapy , 2013 .

[22]  Yaping Li,et al.  Current Approaches of Photothermal Therapy in Treating Cancer Metastasis with Nanotherapeutics , 2016, Theranostics.

[23]  Z. Marković,et al.  In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes. , 2011, Biomaterials.

[24]  Crispin R Dass,et al.  Doxorubicin: an update on anticancer molecular action, toxicity and novel drug delivery systems , 2013, The Journal of pharmacy and pharmacology.

[25]  Xiaoze Shi,et al.  Imaging: PEGylated WS2Nanosheets as a Multifunctional Theranostic Agent for in vivo Dual-Modal CT/Photoacoustic Imaging Guided Photothermal Therapy (Adv. Mater. 12/2014) , 2014 .

[26]  Xiaohua Huang,et al.  Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods. , 2006, Journal of the American Chemical Society.

[27]  W. Wätjen,et al.  Dose- and time-dependent effects of doxorubicin on cytotoxicity, cell cycle and apoptotic cell death in human colon cancer cells. , 2010, Toxicology.

[28]  P. Manivasagan,et al.  Anti-EGFR Antibody Conjugation of Fucoidan-Coated Gold Nanorods as Novel Photothermal Ablation Agents for Cancer Therapy. , 2017, ACS applied materials & interfaces.

[29]  Zhuang Liu,et al.  Carbon nanotubes as photoacoustic molecular imaging agents in living mice. , 2008, Nature nanotechnology.

[30]  G. Pasut,et al.  Polymer-drug conjugation, recent achievements and general strategies , 2007 .

[31]  Stanislav Y. Emelianov,et al.  Biomedical Applications of Photoacoustic Imaging with Exogenous Contrast Agents , 2011, Annals of Biomedical Engineering.

[32]  Jian Song,et al.  A doxorubicin delivery platform using engineered natural membrane vesicle exosomes for targeted tumor therapy. , 2014, Biomaterials.

[33]  J J Lagendijk,et al.  A mathematical model to calculate temperature distributions in human and rabbit eyes during hyperthermic treatment. , 1982, Physics in medicine and biology.

[34]  J. A. Scott.  The computation of temperature rises in the human eye induced by infrared radiation. , 1988, Physics in medicine and biology.

[35]  Sanjiv S Gambhir,et al.  Family of enhanced photoacoustic imaging agents for high-sensitivity and multiplexing studies in living mice. , 2012, ACS nano.

[36]  Nhat Quang Bui,et al.  Doxorubicin-loaded fucoidan capped gold nanoparticles for drug delivery and photoacoustic imaging. , 2016, International journal of biological macromolecules.

[37]  F. Mi,et al.  Development of a new type of multifunctional fucoidan-based nanoparticles for anticancer drug delivery. , 2017, Carbohydrate polymers.

[38]  V. Zharov,et al.  Golden carbon nanotubes as multimodal photoacoustic and photothermal high-contrast molecular agents. , 2009, Nature nanotechnology.

[39]  Xiangliang Yang,et al.  Codelivery of doxorubicin and curcumin with lipid nanoparticles results in improved efficacy of chemotherapy in liver cancer , 2014, International journal of nanomedicine.

[40]  P. Jain,et al.  Calculated absorption and scattering properties of gold nanoparticles of different size, shape, and composition: applications in biological imaging and biomedicine. , 2006, The journal of physical chemistry. B.

[41]  C. Barrett,et al.  Preparation and characterization of polyelectrolyte-coated gold nanoparticles. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[42]  Synthesis and study on activity in vitro of the high purity human butyrylcholinesterase conjugated with gold nanoparticles , 2016, Doklady Biochemistry and Biophysics.

[43]  T. Hahn,et al.  Physicochemical and Biological Characterization of Fucoidan from Fucus vesiculosus Purified by Dye Affinity Chromatography , 2016, Marine drugs.

[44]  M. Wilson,et al.  Comparison of episcleral plaque and proton beam radiation therapy for the treatment of choroidal melanoma. , 1999, Ophthalmology.

[45]  Elodie Boisselier,et al.  Gold nanoparticles in nanomedicine: preparations, imaging, diagnostics, therapies and toxicity. , 2009, Chemical Society reviews.

[46]  D. Shen,et al.  An upconversion nanoparticle--Zinc phthalocyanine based nanophotosensitizer for photodynamic therapy. , 2014, Biomaterials.

[47]  Yan Dai,et al.  Freestanding palladium nanosheets with plasmonic and catalytic properties. , 2011, Nature nanotechnology.

[48]  S. Thomsen.  PATHOLOGIC ANALYSIS OF PHOTOTHERMAL AND PHOTOMECHANICAL EFFECTS OF LASER–TISSUE INTERACTIONS , 1991, Photochemistry and photobiology.

[49]  Jinhui Wu,et al.  Application of near-infrared dyes for tumor imaging, photothermal, and photodynamic therapies. , 2013, Journal of pharmaceutical sciences.

[50]  Kai Yang,et al.  Multimodal Imaging Guided Photothermal Therapy using Functionalized Graphene Nanosheets Anchored with Magnetic Nanoparticles , 2012, Advanced materials.

[51]  P. Finger.  Radiation therapy for choroidal melanoma. , 1997, Survey of ophthalmology.

[52]  S. Nie,et al.  Nanotechnology applications in cancer. , 2007, Annual review of biomedical engineering.

[53]  Lun Ma,et al.  Local field enhanced Au/CuS nanocomposites as efficient photothermal transducer agents for cancer treatment. , 2012, Journal of biomedical nanotechnology.

[54]  D. Gurav,et al.  Synthesis and anticancer properties of fucoidan-mimetic glycopolymer coated gold nanoparticles. , 2015, Chemical communications.

[55]  D. P. O'Neal,et al.  Photo-thermal tumor ablation in mice using near infrared-absorbing nanoparticles. , 2004, Cancer letters.

[56]  S. J. Kang,et al.  Rabbit Model of Retinoblastoma , 2009, Journal of biomedicine & biotechnology.

[57]  Se-Kwon Kim.  Marine Nutraceuticals: Prospects and Perspectives , 2013 .

[58]  Wei Lu,et al.  Copper sulfide nanoparticles for photothermal ablation of tumor cells. , 2010, Nanomedicine.

[59]  B. Hooper.  Optical-thermal response of laser-irradiated tissue , 1996 .

[60]  Hyunkyoung Lee,et al.  Fucoidan from Seaweed Fucus vesiculosus Inhibits Migration and Invasion of Human Lung Cancer Cell via PI3K-Akt-mTOR Pathways , 2012, PloS one.

[61]  Junying Zhang,et al.  CuS nanoagents for photodynamic and photothermal therapies: Phenomena and possible mechanisms. , 2017, Photodiagnosis and photodynamic therapy.

[62]  V. Zharov,et al.  Photothermal nanodrugs: potential of TNF-gold nanospheres for cancer theranostics , 2013, Scientific Reports.