Ion-translocating ATPases in tendrils of Bryonia dioica Jacq.

Procedures have been developed which allow the preparation of highly pure endoplasmic reticulum and plasma membrane from tendrils of Bryonia dioica. These and further membrane fractions were used to study vanadate-sensitive ATPase activity as well as Mg2+ATP-driven transport of 45Ca2+. Calcium-translocating ATPases were detected in the endoplasmic reticulum, the plasma membrane and the mitochondrial fraction and characterized kinetically and with respect to the effects of various inhibitors. The endoplasmic-reticulum Ca2+-translocating ATPase was stimulated by KCl and was calmodulin-dependent. The plasma-membrane enzyme was not affected by these agents. These, as well as the inhibitor data, show that the Ca2+-translocating ATPases of the endoplasmic reticulum and the plasma membrane are distinctly different enzymes. Upon mechanical stimulation, the activities of the vanadate-sensitive K+, Mg2+-ATPase and the Ca2+-translocating ATPase(s) increased rapidly and transiently, indicating that increasing transmembrane proton and calcium fluxes are involved in the early stages of tendril coiling.

[1]  A W Galston,et al.  The Physiology of Tendrils , 1968 .

[2]  P. A. Lanzetta,et al.  An improved assay for nanomole amounts of inorganic phosphate. , 1979, Analytical biochemistry.

[3]  M. Jaffe,et al.  Physiological studies on pea tendrils , 1980 .

[4]  T. Albrecht,et al.  Evidence for the involvement of jasmonates and their octadecanoid precursors in the tendril coiling response of Bryonia dioica , 1993 .

[5]  A W Galston,et al.  Physiological studies on pea tendrils. V. Membrane changes and water movement associated with contact coiling. , 1968, Plant physiology.

[6]  T. Hodges,et al.  Purification of a plasma membrane-bound adenosine triphosphatase from plant roots. , 1974, Methods in enzymology.

[7]  L. Reinhold,et al.  Induction of Coiling in Tendrils by Auxin and Carbon Dioxide , 1967, Science.

[8]  M. I. D. Michelis,et al.  The Ca2+ pump of the plasma membrane of Arabidopsis thaliana: characteristics and sensitivity to fluorescein derivatives , 1993 .

[9]  M. Jaffe.  Physiological Studies on Pea Tendrils: VII. Evaluation of a Technique for the Asymmetrical Application of Ethylene. , 1970, Plant physiology.

[10]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[11]  A. Galston,et al.  Physiological Studies on Pea Tendrils. III. ATPase Activity and Contractility Associated with Coiling. , 1967, Plant physiology.

[12]  D. Arnon.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. , 1949, Plant physiology.

[13]  D. E. Evans,et al.  Reconstitution and Characterization of a Calmodulin-Stimulated Ca-Pumping ATPase Purified from Brassica oleracea L. , 1992, Plant physiology.

[14]  C. Benedetto.  Prostaglandins and related substances : a practical approach , 1987 .

[15]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[16]  T. Singer,et al.  Regulation of Succinate Dehyrogenase in Higher Plants: I. Some General Characteristics of the Membrane-bound Enzyme. , 1973, Plant physiology.

[17]  E Carafoli,et al.  The Ca2+ pump of the plasma membrane. , 1992, The Journal of biological chemistry.

[18]  Rodney H. True,et al.  The Physiology of Tendrils , 1896, Botanical Gazette.

[19]  J. B. Hanson,et al.  Calcium transport and ATPase activity in a microsomal vesicle fraction from corn roots , 1983 .

[20]  H. Greppin,et al.  Calcium uptake by tonoplast and plasma membrane vesicles from spinach leaves , 1988 .

[21]  A. Galston,et al.  Physiological Studies on Pea Tendrils. II. The Role of Light and ATP in Contact Coiling. , 1966, Plant physiology.

[22]  E. Weiler,et al.  Characterization and localization of fusicoccin-binding sites in leaf tissues of Vicia faba L. probed with a novel radioligand , 1988, Planta.

[23]  E. Weiler,et al.  Methyljasmonate and α-linolenic acid are potent inducers of tendril coiling , 1991, Planta.

[24]  H. Gross,et al.  Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels , 1987 .

[25]  H. Sze,et al.  Calcium-pumping ATPases in vesicles from carrot cells : stimulation by calmodulin or phosphatidylserine, and formation of a 120 kilodalton phosphoenzyme. , 1991, Plant physiology.

[26]  E. Weiler,et al.  Fusicoccin action in cell-suspension cultures of Corydalis sempervirens Pers. , 1991, Planta.

[27]  A W Galston,et al.  Physiological studies on pea tendrils. I. Growth and coiling following mechanical stimulation. , 1966, Plant physiology.

[28]  L. Williams,et al.  Investigation of the Calcium-Transporting ATPases at the Endoplasmic Reticulum and Plasma Membrane of Red Beet (Beta vulgaris) , 1993, Plant physiology.

[29]  D. P. Briskin.  Ca-translocating ATPase of the plant plasma membrane. , 1990, Plant physiology.

[30]  Ronald W. Davis,et al.  Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis , 1990, Cell.

[31]  G. D. Jaegher,et al.  Microsomal ATP-dependent Ca2+ transport as affected by environmental stress in Bryonia dioica internodes , 1991 .

[32]  E. Weiler,et al.  ATP-driven Ca2+ transport in sealed plasma membrane vesicles prepared by aqueous two-phase partitioning from leaves of Commelina communis , 1989 .

[33]  E. Weiler,et al.  Characterization and purification of the fusicoccin-binding complex from plasma membranes of Commelina communis. , 1991, European journal of biochemistry.

[34]  H. Sze,et al.  A Plasma Membrane-Type Ca2+-ATPase of 120 Kilodaltons on the Endoplasmic Reticulum from Carrot (Daucus carota) Cells (Properties of the Phosphorylated Intermediate) , 1993, Plant physiology.

[35]  A. Campbell,et al.  Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium , 1991, Nature.

[36]  A. Hager,et al.  A Ca2+-ATPase and a Mg2+/H+-antiporter are present on tonoplast membranes from roots of Zea mays L. , 1993, Planta.