Biological hydrogen and methane production from bagasse bioethanol fermentation residues using a two-stage bioprocess.

This study investigated the recovery of H2 and CH4 from bagasse bioethanol fermentation residues (bagasse BEFR) using a two-stage bioprocess. In the hydrogen fermentation bioreactor (HFB), carbohydrate removal efficiency was maintained at 82-93% and the highest hydrogen yield was 8.24mL/gCOD at volumetric loading rate (VLR) of 80kgCOD/m(3)/day. The results indicated a positive correlation between hydrogen yield and butyrate-to-acetate ratio, which might be due to the mechanisms of lactate/acetate utilization for hydrogen production and acetogenesis occurring in the HFB. Remaining volatile fatty acids and alcohols in the HFB effluent were further utilized for methane production in methane fermentation bioreactor (MFB), in which the highest methane yield of 345.2mL/gCOD was attained at VLR of 2.5kgCOD/m(3)/day. Overall, the two-stage bioprocess achieved a maximum COD removal of 81% from bagasse BEFR, and converted 0.3% and 72.8% of COD in the forms of H2 and CH4, respectively.

[1]  P. Mccarty,et al.  Bioassay for monitoring biochemical methane potential and anaerobic toxicity , 1979 .

[2]  L. Whang,et al.  Metabolic flux network analysis of fermentative hydrogen production: using Clostridium tyrobutyricum as an example. , 2013, Bioresource technology.

[3]  Hyung-Sool Lee,et al.  Biological hydrogen production: prospects and challenges. , 2010, Trends in biotechnology.

[4]  L. Whang,et al.  Metabolic and energetic aspects of biohydrogen production of Clostridium tyrobutyricum: The effects of hydraulic retention time and peptone addition. , 2011, Bioresource technology.

[5]  Tian C. Zhang,et al.  Comparison of One-Phase and Two-Phase Anaerobic Digestion Processes in Characteristics of Substrate Degradation and Bacterial Population Levels , 1991 .

[6]  Bernardo Ruggeri,et al.  Energy balance of dark anaerobic fermentation as a tool for sustainability analysis , 2010 .

[7]  Fermentative biohydrogen production from lactate and acetate. , 2012, Bioresource technology.

[8]  H. Hou,et al.  Co-producing hydrogen and methane from higher-concentration of corn stalk by combining hydrogen fermentation and anaerobic digestion , 2014 .

[9]  H. Kübler,et al.  Three-phase anaerobic digestion of organic wastes , 1994 .

[10]  Jean-Philippe Steyer,et al.  Sub-dominant bacteria as keystone species in microbial communities producing bio-hydrogen , 2013 .

[11]  Yu-You Li,et al.  Effect of organic loading rate on continuous hydrogen production from food waste in submerged anaerobic membrane bioreactor , 2014 .

[12]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[13]  Sheng-Shung Cheng,et al.  A dual‐substrate steady‐state model for biological hydrogen production in an anaerobic hydrogen fermentation process , 2006, Biotechnology and bioengineering.

[14]  Xiaohui Xu,et al.  Hydrogen and methane production from lipid-extracted microalgal biomass residues , 2011 .

[15]  Min Ji,et al.  Hydrogen and methane production by co-digestion of waste activated sludge and food waste in the two-stage fermentation process: substrate conversion and energy yield. , 2013, Bioresource technology.

[16]  Dae Sung Lee,et al.  Statistical optimization of key process variables for enhanced hydrogen production by newly isolated Clostridium tyrobutyricum JM1 , 2008 .

[17]  Patrick C. Hallenbeck,et al.  Fermentative hydrogen production: Principles, progress, and prognosis , 2009 .

[18]  G. Nakhla,et al.  The effect of heat pretreatment temperature on fermentative hydrogen production using mixed cultures , 2008 .

[19]  Jianquan Shen,et al.  Effects of culture and medium conditions on hydrogen production from starch using anaerobic bacteria. , 2004, Journal of bioscience and bioengineering.

[20]  B. Rittmann,et al.  Evaluation of metabolism using stoichiometry in fermentative biohydrogen , 2009, Biotechnology and bioengineering.

[21]  Dae Sung Lee,et al.  Biological hydrogen production by immobilized cells of Clostridium tyrobutyricum JM1 isolated from a food waste treatment process. , 2008, Bioresource technology.

[22]  Chun-Po Juang,et al.  Evaluation of bioenergy recovery processes treating organic residues from ethanol fermentation process. , 2011, Bioresource technology.

[23]  Michael Kornaros,et al.  Biohydrogen and methane production from cheese whey in a two-stage anaerobic process , 2008 .

[24]  R. Thauer,et al.  Energy Conservation in Chemotrophic Anaerobic Bacteria , 1977, Bacteriological reviews.

[25]  L. Whang,et al.  A two-stage bioprocess for hydrogen and methane production from rice straw bioethanol residues. , 2012, Bioresource technology.

[26]  A Polettini,et al.  A review of dark fermentative hydrogen production from biodegradable municipal waste fractions. , 2013, Waste management.

[27]  John R. Benemann,et al.  Biological hydrogen production , 1995 .

[28]  M. Matsumoto,et al.  Hydrogen production by fermentation using acetic acid and lactic acid. , 2007, Journal of bioscience and bioengineering.

[29]  Sang-Eun Oh,et al.  Biohydrogen gas production from food processing and domestic wastewaters , 2005 .

[30]  I. Sakamoto,et al.  Microbial diversity of hydrogen-producing bacteria in batch reactors fed with cellulose using leachate as inoculum , 2013 .

[31]  K. Schleifer,et al.  The Prokaryotes. A handbook on the biology of bacteria: ecophysiology, isolation, identification, applications. Volumes I-IV. , 1992 .

[32]  B. Kasapgil,et al.  Microbiological study of two-stage anaerobic digestion during start-up , 1994 .

[33]  Alan J Guwy,et al.  Fermentative production of hydrogen from a wheat flour industry co-product. , 2008, Bioresource technology.

[34]  Patrick C. Hallenbeck,et al.  Biological hydrogen production; fundamentals and limiting processes , 2002 .