Protective effects of tumor necrosis factor α antibody and ulinastatin on liver ischemic reperfusion in rats

AIM To study the protective effects of tumor necrosis factor alpha (TNF alpha) antibody and ulinastatin on liver ischemic reperfusion in rats. METHODS One hundred and twenty male SD rats were randomly divided into four groups: normal control group, ischemic group, TNF alpha antibody group and TNF alpha antibody + ulinastatin group. The animals were killed at 0, 3, 6, 9, 12 h after ischemia for 60 min and followed by reperfusion. Serum alanine aminotransferase (ALT), malondialdehyde (MDA) and liver histopathology were observed. RESULTS After ischemic reperfusion, the serum ALT and MDA were remarkably increased, and the hepatic congestion was obvious. Treatment of TNF alpha antibody and ulinastatin could significantly decrease serum ALT and MDA levels, and relieve hepatic congestion. CONCLUSION Ulinastatin and TNF alpha antibody can suppress the inflammatory reaction induced by hepatic ischemic reperfusion, and have protective effects on rat hepatic ischemic reperfusion injury.

[1]  R. Mehvar,et al.  Attenuation of Kupffer Cell Activation in Cold-Preserved Livers After Pretreatment of Rats with Methylprednisolone or Its Macromolecular Prodrug , 2003, Pharmaceutical Research.

[2]  S. Kawachi,et al.  Role of nitric oxide in liver ischemia and reperfusion injury , 2002, Molecular and Cellular Biochemistry.

[3]  G. Farrell,et al.  Hepatic ischemia reperfusion injury: Pathogenic mechanisms and basis for hepatoprotection , 2003, Journal of gastroenterology and hepatology.

[4]  K. Asadullah,et al.  Cytokine and anticytokine therapy in dermatology , 2003, Expert opinion on biological therapy.

[5]  H. Nagasawa,et al.  Protective effect of urinary trypsin inhibitor on myocardial mitochondria during hemorrhagic shock and reperfusion , 2003, Critical care medicine.

[6]  H. D. Liggitt,et al.  Sustained Tolerance to Lipopolysaccharide After Liver Ischemia-Reperfusion Injury , 2003, Shock.

[7]  A. Kakita,et al.  Pyrrolidine dithiocarbamate provides protection against hypothermic preservation and transplantation injury in the rat liver: the role of heme oxygenase-1. , 2003, Surgery.

[8]  Chao-Long Chen,et al.  The protective mechanism of magnolol, a Chinese herb drug, against warm ischemia-reperfusion injury of rat liver. , 2003, The Journal of surgical research.

[9]  R. Busuttil,et al.  Hepatic ischemia/reperfusion injury--a fresh look. , 2003, Experimental and molecular pathology.

[10]  Yitao Ding,et al.  Matrine protects sinusoidal endothelial cells from cold ischemia and reperfusion injury in rat orthotopic liver transplantation. , 2003, Annals of clinical and laboratory science.

[11]  J. Devière,et al.  Preconditioning of donors with interleukin-10 reduces hepatic ischemia-reperfusion injury after liver transplantation in pigs , 2003, Transplantation.

[12]  M. Ozaki,et al.  Effect of ulinastatin on microcirculation during excessive hemorrhage using fluid therapy. , 2003, In vivo.

[13]  K. Ushijima,et al.  Neuroprotective Effect of Urinary Trypsin Inhibitor against Focal Cerebral Ischemia–Reperfusion Injury in Rats , 2003, Anesthesiology.

[14]  T. Iwaya,et al.  Influence of a Human Protease Inhibitor on Surgical Stress Induced Immunosuppression , 2012, Digestive Surgery.

[15]  W. Banks,et al.  Entry of Blood-Borne Cytokines into the Central Nervous System: Effects on Cognitive Processes , 2003, Neuroimmunomodulation.

[16]  Ruben Zamora,et al.  A DNA microarray study of nitric oxide-induced genes in mouse hepatocytes: implications for hepatic heme oxygenase-1 expression in ischemia/reperfusion. , 2002, Nitric oxide : biology and chemistry.

[17]  G. Poli,et al.  Microvascular dysfunction induced by reperfusion injury and protective effect of ischemic preconditioning. , 2002, Free radical biology & medicine.

[18]  A. Lentsch,et al.  Specific role of interleukin-1 in hepatic neutrophil recruitment after ischemia/reperfusion. , 2002, The American journal of pathology.

[19]  A. Gambotto,et al.  Role of NF-κB on liver cold ischemia-reperfusion injury , 2002 .

[20]  J. Geng,et al.  Effect of N-desulfated heparin on hepatic/renal ischemia reperfusion injury in rats. , 2002, World journal of gastroenterology.

[21]  Y. Iwasaki,et al.  Protective effect of ischemic preconditioning against intermittent warm-ischemia-induced liver injury. , 2002, The Journal of surgical research.

[22]  S. Matsuda,et al.  c‐Jun N‐terminal kinase activation during warm hepatic ischemia/reperfusion injuries in a rat model , 2002, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[23]  A. Kakita,et al.  Protective Effects of Urinary Trypsin Inhibitor (UTI) on Hepatic Microvasculature in Hypotensive Brain-Dead Rats , 2002, European Surgical Research.

[24]  Y. Hwang,et al.  Hepatocyte protection by a protease inhibitor against ischemia/reperfusion injury of human liver. , 2002, Journal of the American College of Surgeons.

[25]  Y. Hwang,et al.  Enhanced inflammatory cytokine production at ischemia/reperfusion in human liver resection. , 2002, Hepato-Gastroenterology.

[26]  A. Lentsch,et al.  Gene deletion of NF-kappa B p50 does not alter the hepatic inflammatory response to ischemia/reperfusion. , 2002, Journal of hepatology.

[27]  B. Vidne,et al.  Role of anti-tumor necrosis factor-alpha in ischemia/reperfusion injury in isolated rat liver in a blood-free environment. , 2002, Transplantation.

[28]  M. Miyazaki,et al.  Effect of cold-ischemia time on C-X-C chemokine expression and neutrophil accumulation in the graft liver after orthotopic liver transplantation in rats , 2002, Transplantation.

[29]  R. Bartrons,et al.  The combination of ischemic preconditioning and liver Bcl-2 overexpression is a suitable strategy to prevent liver and lung damage after hepatic ischemia-reperfusion. , 2002, The American journal of pathology.

[30]  M. Uchiba,et al.  Ischemia/reperfusion-induced increase in the hepatic level of prostacyclin is mainly mediated by activation of capsaicin-sensitive sensory neurons in rats. , 2002, The Journal of laboratory and clinical medicine.

[31]  J. Lott,et al.  Clinical utility of a rapid test for uristatin. , 2002, Clinical Biochemistry.

[32]  Y. Takikawa,et al.  Plasma and urine levels of urinary trypsin inhibitor in patients with acute and fulminant hepatitis , 2002, Journal of gastroenterology and hepatology.

[33]  A. Gambotto,et al.  Role of NF-kappaB on liver cold ischemia-reperfusion injury. , 2002, American Journal of Physiology - Gastrointestinal and Liver Physiology.

[34]  K. Suzuki,et al.  Two patients with severe alcoholic hepatitis accompanied by hypercytokinemia and granulocytic hyperelastasemia, successfully treated by intravenous infusion of urinastarine (Miraclid) , 2001, Journal of gastroenterology and hepatology.

[35]  T. Junginger,et al.  Calcium prevents loss of glutathione and reduces oxidative stress upon reperfusion in the perfused liver. , 2000, International Journal of Surgical Investigation.

[36]  J. Koudelová,et al.  [Adrenergic tocolytics--their effects on lipoperoxidation in the brain]. , 1997, Ceska gynekologie.