Focal adhesions - the cytoskeletal connection.

Cellular contacts with the extracellular matrix are regulated by the Rho family of GTPases through their effects on both the actin and the microtubule cytoarchitecture. Recent genetic, biochemical and structural data have highlighted the role played by a subset of actin-binding proteins in coupling integrins to cytoskeletal actin and in assembling signalling complexes that are important for cell motility and cell proliferation.

[1]  Rangarajan Sampath,et al.  Cytoskeletal Interactions with the Leukocyte Integrin β2 Cytoplasmic Tail , 1998, The Journal of Biological Chemistry.

[2]  A. Gilmore,et al.  Regulation of vinculin binding to talin and actin by phosphatidyl-inositol-4-5-bisphosphate , 1996, Nature.

[3]  D. Critchley,et al.  Talin contains three similar vinculin-binding sites predicted to form an amphipathic helix , 1999 .

[4]  Z. Kam,et al.  Focal adhesion formation by F9 embryonal carcinoma cells after vinculin gene disruption. , 1995, Journal of cell science.

[5]  Tina M. Leisner,et al.  Direct Binding of the Platelet Integrin αIIbβ3 (GPIIb-IIIa) to Talin , 1996, The Journal of Biological Chemistry.

[6]  B. Jockusch,et al.  The ultrastructure of chicken gizzard vinculin as visualized by high-resolution electron microscopy. , 1996, Journal of structural biology.

[7]  B. Kay,et al.  A Role for CAP, a Novel, Multifunctional Src Homology 3 Domain-containing Protein in Formation of Actin Stress Fibers and Focal Adhesions* , 1998, The Journal of Biological Chemistry.

[8]  I. Kanazawa,et al.  HIP1, a human homologue of S. cerevisiae Sla2p, interacts with membrane-associated huntingtin in the brain , 1997, Nature Genetics.

[9]  William B Dobyns,et al.  Mutations in filamin 1 Prevent Migration of Cerebral Cortical Neurons in Human Periventricular Heterotopia , 1998, Neuron.

[10]  S. Kanner,et al.  Filamin binds to the cytoplasmic domain of the beta1-integrin. Identification of amino acids responsible for this interaction. , 1998, The Journal of biological chemistry.

[11]  M. Tremblay,et al.  Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts , 1999, The Journal of cell biology.

[12]  Y. Takai,et al.  Ponsin/SH3P12: An l-Afadin– and Vinculin-binding Protein Localized at Cell–Cell and Cell–Matrix Adherens Junctions , 1999, The Journal of cell biology.

[13]  O. Hobert,et al.  SH3 domain-dependent interaction of the proto-oncogene product Vav with the focal contact protein zyxin. , 1996, Oncogene.

[14]  H. Lee,et al.  Interaction of talin with actin: sensitive modulation of filament crosslinking activity. , 1999, Archives of biochemistry and biophysics.

[15]  S. Aota,et al.  Molecular diversity of cell-matrix adhesions. , 1999, Journal of cell science.

[16]  K. Burridge,et al.  Functional studies of the domains of talin , 1990, The Journal of cell biology.

[17]  P. Janmey,et al.  The Role of Actin-binding Protein 280 in Integrin-dependent Mechanoprotection* , 1998, The Journal of Biological Chemistry.

[18]  W. Goldmann,et al.  Peptide‐specific antibodies localize the major lipid binding sites of talin dimers to oppositely arranged N‐terminal 47 kDa subdomains 1 , 1998, FEBS letters.

[19]  K. Yahata,et al.  A unique talin homologue with a villin headpiece-like domain is required for multicellular morphogenesis in Dictyostelium , 1999, Current Biology.

[20]  B. Geiger,et al.  Overexpression of vinculin suppresses cell motility in BALB/c 3T3 cells. , 1992, Cell motility and the cytoskeleton.

[21]  Clive R. Bagshaw,et al.  Crystal Structure of the Vinculin Tail Suggests a Pathway for Activation , 1999, Cell.

[22]  S. Craig,et al.  The I/LWEQ module: a conserved sequence that signifies F-actin binding in functionally diverse proteins from yeast to mammals. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[23]  A. Woods,et al.  Disruption of the Talin Gene Compromises Focal Adhesion Assembly in Undifferentiated but Not Differentiated Embryonic Stem Cells , 1998, The Journal of cell biology.

[24]  R. Insall,et al.  Signaling to Actin Dynamics , 1999, The Journal of cell biology.

[25]  P. Janmey,et al.  Actin-binding protein requirement for cortical stability and efficient locomotion. , 1992, Science.

[26]  E. Adamson,et al.  Vinculin knockout results in heart and brain defects during embryonic development. , 1998, Development.

[27]  K. Vuori.  Integrin Signaling: Tyrosine Phosphorylation Events in Focal Adhesions , 1998, The Journal of Membrane Biology.

[28]  S. Aizawa,et al.  Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice , 1995, Nature.

[29]  S. Barry,et al.  ARF1 Mediates Paxillin Recruitment to Focal Adhesions and Potentiates Rho-stimulated Stress Fiber Formation in Intact and Permeabilized Swiss 3T3 Fibroblasts , 1998, The Journal of cell biology.

[30]  R. Fässler,et al.  Consequences of lack of beta 1 integrin gene expression in mice. , 1995, Genes & development.

[31]  Y. Ono,et al.  Interaction of PKN with α-Actinin* , 1997, The Journal of Biological Chemistry.

[32]  V. Niggli,et al.  A conserved motif in the tail domain of vinculin mediates association with and insertion into acidic phospholipid bilayers. , 1998, Biochemistry.

[33]  R. Hynes,et al.  Layilin, A Novel Talin-binding Transmembrane Protein Homologous with C-type Lectins, is Localized in Membrane Ruffles , 1998, The Journal of cell biology.

[34]  B. Jockusch,et al.  Characterization of two F-actin-binding and oligomerization sites in the cell-contact protein vinculin. , 1997, European journal of biochemistry.

[35]  N. Adey,et al.  Polyphosphoinositides Inhibit the Interaction of Vinculin with Actin Filaments* , 1999, The Journal of Biological Chemistry.

[36]  Kenneth M. Yamada,et al.  Vinexin: A Novel Vinculin-binding Protein with Multiple SH3 Domains Enhances Actin Cytoskeletal Organization , 1999, The Journal of cell biology.

[37]  D. Erle,et al.  Integrin β Cytoplasmic Domains Differentially Bind to Cytoskeletal Proteins* , 1998, The Journal of Biological Chemistry.

[38]  S. Barry,et al.  Acidic phospholipids inhibit the intramolecular association between the N- and C-terminal regions of vinculin, exposing actin-binding and protein kinase C phosphorylation sites. , 1996, The Biochemical journal.

[39]  Thomas Kreis,et al.  Guidebook to cytoskeletal and motor proteins , 1999 .

[40]  M. Beckerle,et al.  Interaction of plasma membrane fibronectin receptor with talin—a transmembrane linkage , 1986, Nature.

[41]  R. Hynes,et al.  The Talin Head Domain Binds to Integrin β Subunit Cytoplasmic Tails and Regulates Integrin Activation* , 1999, The Journal of Biological Chemistry.

[42]  R. Hynes,et al.  Talin contains three actin-binding sites each of which is adjacent to a vinculin-binding site. , 1996, Journal of cell science.

[43]  M. Brown,et al.  Serine and threonine phosphorylation of the paxillin LIM domains regulates paxillin focal adhesion localization and cell adhesion to fibronectin. , 1998, Molecular biology of the cell.

[44]  D. Schlaepfer,et al.  Required role of focal adhesion kinase (FAK) for integrin-stimulated cell migration. , 1999, Journal of cell science.

[45]  M. Schwartz,et al.  Integrin-dependent tyrosine phosphorylation and growth regulation by Vav. , 1999, Cell adhesion and communication.

[46]  J. Cloutier,et al.  Direct Association of Protein-tyrosine Phosphatase PTP-PEST with Paxillin* , 1998, The Journal of Biological Chemistry.

[47]  W. H. Goldmann,et al.  Probing actin and liposome interaction of talin and talin-vinculin complexes: a kinetic, thermodynamic and lipid labeling study. , 1992, Biochemistry.

[48]  K. Tedford,et al.  Vav is a regulator of cytoskeletal reorganization mediated by the T-cell receptor , 1998, Current Biology.

[49]  L. Silengo,et al.  beta1-integrin cytoplasmic subdomains involved in dominant negative function. , 1998, Molecular biology of the cell.

[50]  K. Burridge,et al.  Bidirectional signaling between the cytoskeleton and integrins. , 1999, Current opinion in cell biology.

[51]  T. Jarchau,et al.  The interaction of the cell-contact proteins VASP and vinculin is regulated by phosphatidylinositol-4,5-bisphosphate , 1998, Current Biology.

[52]  C. Turner,et al.  Paxillin LD4 Motif Binds PAK and PIX through a Novel 95-kD Ankyrin Repeat, ARF–GAP Protein: A Role in Cytoskeletal Remodeling , 1999, The Journal of cell biology.

[53]  B. Geiger,et al.  Suppression of vinculin expression by antisense transfection confers changes in cell morphology, motility, and anchorage-dependent growth of 3T3 cells , 1993, The Journal of cell biology.

[54]  R L Juliano,et al.  Integrin signaling and cell growth control. , 1998, Current opinion in cell biology.