The structural correlate of the receptive field centre of alpha ganglion cells in the cat retina.

The correlation between the receptive field centre and the dendritic tree of individual brisk transient, or alpha, ganglion cells in the cat retina was investigated by a combination of physiological and anatomical techniques. The sizes of receptive field centres of brisk transient (Y) cells were measured with a flickering spot of light. Contour maps and response (or sensitivity) profiles were measured at mesopic and scotopic backgrounds. Recording positions on the retina and nearby blood vessels were back‐projected onto the receptive field plots on the tangent screen. After recording, whole amount preparations of the retinae were stained by a reduced silver method to stain all alpha cells together with their dendritic trees. By comparing the landmarks on the screen plot with those of the whole mount it was possible to identify the recorded cells in the preparation and to study their morphology. The dendritic tree of an alpha cell determines the position, size and shape of its receptive field centre. The mesopic receptive field centres were found to be a factor of 1.4 +/‐ 0.13 larger than their respective dendritic fields. It is suggested that the dendritic fields of presynaptic neurones (bipolar and amacrine cell processes) add to the ganglion cell dendritic tree to produce the larger centre summating area.

[1]  B. Boycott,et al.  Organization of the primate retina: electron microscopy , 1966, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[2]  B. Boycott,et al.  Morphology and topography of on- and off-alpha cells in the cat retina , 1981, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[3]  B. Boycott,et al.  The morphological types of ganglion cells of the domestic cat's retina , 1974, The Journal of physiology.

[4]  H. Wässle,et al.  Size, scatter and coverage of ganglion cell receptive field centres in the cat retina. , 1979, The Journal of physiology.

[5]  E. V. Famiglietti,et al.  Functional architecture of cone bipolar cells in mammalian retina , 1981, Vision Research.

[6]  S. W. Kuffler.  Discharge patterns and functional organization of mammalian retina. , 1953, Journal of neurophysiology.

[7]  Max Johann Sigismund Schultze,et al.  Zur Anatomie und Physiologie der Retina , 1866 .

[8]  W. Levick,et al.  Brisk and sluggish concentrically organized ganglion cells in the cat's retina , 1974, The Journal of physiology.

[9]  W. R. Levick,et al.  Form and function of cat retinal ganglion cells , 1975, Nature.

[10]  T. Poggio,et al.  Retinal ganglion cells: a functional interpretation of dendritic morphology. , 1982, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[11]  C. Enroth-Cugell,et al.  Light distribution in the cat's retinal image , 1978, Vision Research.

[12]  B. Boycott,et al.  Morphological identification of on- and off-centre brisk transient (Y) cells in the cat retina. , 1981, Proceedings of the Royal Society of London. Series B, Biological sciences.

[13]  H. Kolb.  The inner plexiform layer in the retina of the cat: electron microscopic observations , 1979, Journal of neurocytology.

[14]  R. Nelson,et al.  Cat cones have rod input: A comparison of the response properties of cones and horizontal cell bodies in the retina of the cat , 1977, The Journal of comparative neurology.

[15]  D. P. Andrews,et al.  Suprathreshold spectral properties of single optic tract fibres in cat, under mesopic adaptation; cone—rod interaction , 1970, The Journal of physiology.

[16]  P. Sterling.  Microcircuitry of the cat retina. , 1983, Annual review of neuroscience.

[17]  P. Sterling,et al.  Toward a functional architecture of the retina: serial reconstruction of adjacent ganglion cells. , 1980, Science.

[18]  H. Barlow,et al.  Change of organization in the receptive fields of the cat's retina during dark adaptation , 1957, The Journal of physiology.

[19]  Helga Kolb,et al.  Amacrine cells, bipolar cells and ganglion cells of the cat retina: A Golgi study , 1981, Vision Research.

[20]  R. W. Rodieck,et al.  Analysis of receptive fields of cat retinal ganglion cells. , 1965, Journal of neurophysiology.

[21]  H. Wässle,et al.  Morphological identification of on- and off-centre brisk transient (Y) cells in the cat retina , 1981, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[22]  D. Major,et al.  Cat retinal ganglion cell dendritic fields. , 1966, Experimental neurology.

[23]  E. V. Famiglietti,et al.  Structural basis for ON-and OFF-center responses in retinal ganglion cells. , 1976, Science.

[24]  B. Boycott,et al.  The connections between bipolar cells and photoreceptors in the retina of the domestic cat , 1973, The Journal of comparative neurology.

[25]  H. Kolb,et al.  Intracellular staining reveals different levels of stratification for on- and off-center ganglion cells in cat retina. , 1978, Journal of neurophysiology.

[26]  P. Hammond.  Inadequacy of nitrous oxide/oxygen mixtures for maintaining anaesthesia in cats: satisfactory alternatives , 1978, Pain.

[27]  J. Stone,et al.  Ganglion, amacrine and horizontal cells of the cat's retina. , 1967, Vision research.

[28]  B. Sakmann,et al.  Sensitivity distribution and spatial summation within receptive-field center of retinal on-center ganglion cells and transfer function of the retina. , 1970, Journal of neurophysiology.