Characterization, antioxidant and cytotoxicity evaluation of green synthesized silver nanoparticles using Cleistanthus collinus extract as surface modifier

Graphical abstract: The figure is the TEM image of green synthesized silver nanoparticles from Cleistanthus collinus. In this investigation we have used the poisonous plant as a reducing and capping agent. This is a first time data to synthesis the metal nanoparticles using poisonous plant. - Highlights: • A hitherto unreported venomous plant mediated AgNPs synthesis. • The particle size is observed in the range of 20–40 nm. • Surface morphology of the well-dispersed silver nanoparticles is studied using SEM and TEM. • Crystalline nature of AgNPs is confirmed by X-ray diffraction analysis. • Antioxidant activities of green synthesized AgNPs are tested in vitro. - Abstract: We report, here a simple green method for the preparation of silver nanoparticles (AgNPs) using the plant extract of Cleistanthus collinus as potential phyto reducer. The synthesized AgNPs were characterized by UV–vis spectra, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The obtained results confirmed that the AgNPs were crystalline in nature and the morphological studies reveal the spherical shape of AgNPs with size ranging from 20 to 40 nm. The in vitro antioxidant activity of AgNPs showed a significant effect on scavenging of freemore » radicals. The cytotoxicity study exhibited a dose-dependent effect against human lung cancer cells (A549) and normal cells (HBL-100), the inhibitory concentration (IC{sub 50}) were found to be 30 μg/mL and 60 μg/mL respectively. The in vivo histopathology of mouse organs proved that AgNPs does not possess toxic effect and can be extensively applied in biomedical sciences.« less

[1]  Dejian Huang,et al.  The chemistry behind antioxidant capacity assays. , 2005, Journal of agricultural and food chemistry.

[2]  N. Hamasaki,et al.  Mitochondrial Oxidative Stress and Mitochondrial DNA , 2003, Clinical chemistry and laboratory medicine.

[3]  J. Antony,et al.  Cytotoxic effect of Green synthesized silver nanoparticles using Melia azedarach against in vitro HeLa cell lines and lymphoma mice model , 2012 .

[4]  R. Pleixats,et al.  Formation of carbon--carbon bonds under catalysis by transition-metal nanoparticles. , 2003, Accounts of chemical research.

[5]  W. D. de Jong,et al.  Nano-silver – a review of available data and knowledge gaps in human and environmental risk assessment , 2009 .

[6]  Absar Ahmad,et al.  Biosynthesis of gold and silver nanoparticles using Emblica Officinalis fruit extract, their phase transfer and transmetallation in an organic solution. , 2005, Journal of nanoscience and nanotechnology.

[7]  Amy Milsted,et al.  Silver(I)-imidazole cyclophane gem-diol complexes encapsulated by electrospun tecophilic nanofibers: formation of nanosilver particles and antimicrobial activity. , 2005, Journal of the American Chemical Society.

[8]  Beom Soo Kim,et al.  Rapid biological synthesis of silver nanoparticles using plant leaf extracts , 2009, Bioprocess and biosystems engineering.

[9]  S. Zinjarde,et al.  Banana peel extract mediated novel route for the synthesis of silver nanoparticles , 2010 .

[10]  T. Hatano,et al.  Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. , 1988, Chemical & pharmaceutical bulletin.

[11]  T. Osawa,et al.  Hydroxyl Radical-scavenging Effects of Spices and Scavengers from Brown Mustard (Brassica nigra) , 1997 .

[12]  C. N. R. Rao,et al.  Science and technology of nanomaterials: current status and future prospects , 2001 .

[13]  S. Gurunathan,et al.  Biosynthesis of silver nanocrystals by Bacillus licheniformis. , 2008, Colloids and surfaces. B, Biointerfaces.

[14]  R. Thangam,et al.  Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cells , 2012 .

[15]  Absar Ahmad,et al.  Role of halide ions and temperature on the morphology of biologically synthesized gold nanotriangles. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[16]  M. Oyaizu.  Studies on products of browning reaction--antioxidative activities of products of browning reaction prepared from glucosamine , 1986 .

[17]  M. Jeyaraj,et al.  Biogenic silver nanoparticles for cancer treatment: an experimental report. , 2013, Colloids and surfaces. B, Biointerfaces.

[18]  E Olsson,et al.  Silver-based crystalline nanoparticles, microbially fabricated. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[19]  Hari Singh Nalwa,et al.  Handbook of nanostructured materials and nanotechnology , 2000 .

[20]  A. Henglein.  Physicochemical properties of small metal particles in solution: "microelectrode" reactions, chemisorption, composite metal particles, and the atom-to-metal transition , 1993 .

[21]  Ahmad Reza Shahverdi,et al.  Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: A novel biological approach , 2007 .

[22]  V. Poon,et al.  Antimicrobial activities of silver dressings: an in vitro comparison. , 2006, Journal of medical microbiology.

[23]  R. Sanghi,et al.  Biomimetic synthesis and characterisation of protein capped silver nanoparticles. , 2009, Bioresource technology.

[24]  Jiale Huang,et al.  Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf , 2007 .

[25]  H. Matsumoto,et al.  Formation of Colloidal Gold Nanoparticles in an Ultrasonic Field: Control of Rate of Gold(III) Reduction and Size of Formed Gold Particles , 2001 .

[26]  R. Naik,et al.  Biomimetic synthesis and patterning of silver nanoparticles , 2002, Nature materials.

[27]  Ajay Misra,et al.  Green synthesis of silver nanoparticles using seed extract of Jatropha curcas , 2009 .

[28]  R. Thangam,et al.  A novel disintegrin protein from Naja naja venom induces cytotoxicity and apoptosis in human cancer cell lines in vitro , 2012 .

[29]  E. Pick,et al.  Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader. , 1981, Journal of immunological methods.

[30]  Absar Ahmad,et al.  Synthesis of Gold Nanotriangles and Silver Nanoparticles Using Aloevera Plant Extract , 2006, Biotechnology progress.

[31]  B. Cullity,et al.  Elements of X-ray diffraction , 1957 .

[32]  Kumar,et al.  Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum , 2003 .

[33]  Sudhakar R. Sainkar,et al.  Fungus-mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix: a novel biological approach to nanoparticle synthesis , 2001 .

[34]  S. Dhara,et al.  Electrical transport studies of Ag nanoclusters embedded in glass matrix , 2001 .

[35]  Absar Ahmad,et al.  Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. , 2004, Journal of colloid and interface science.