Identification of collagen-based materials in cultural heritage.

All stakeholders in cultural heritage share an interest in fabrication methods and material technology. Until now methods for analysis of organic materials, particularly proteins, have not been widely available to researchers at cultural institutions. This paper will describe an analytical method for the identification of collagen-based materials from soft tissue sources and show examples of its application to diverse museum objects. The method, peptide mass fingerprinting (PMF), uses enzymatic digestion of extracted proteins to produce a mixture of peptides. The mass spectrum of the mixture contains characteristic marker ions-a peptide mass fingerprint-which are compared to species-specific markers from references as the basis of identification. Preliminary results indicate that analysis of materials from aged samples, several different tissue types, and tanned or untanned materials yields comparable PMF results. Significantly, PMF is simple, rapid, sensitive and specific, has been implemented in a museum laboratory, and is being practiced successfully by non-specialists.

[1]  Martin Strohalm,et al.  mMass data miner: an open source alternative for mass spectrometric data analysis. , 2008, Rapid communications in mass spectrometry : RCM.

[2]  J. Allison,et al.  Identification of colorants as used in watercolor and oil paintings by UV laser desorption mass spectrometry , 2003 .

[3]  John T. Stults,et al.  Protein identification: The origins of peptide mass fingerprinting , 2003, Journal of the American Society for Mass Spectrometry.

[4]  Michael Buckley,et al.  Species identification by analysis of bone collagen using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. , 2009, Rapid communications in mass spectrometry : RCM.

[5]  Antonio Sgamellotti,et al.  Proteomic strategies for the identification of proteinaceous binders in paintings , 2009, Analytical and bioanalytical chemistry.

[6]  M. Colombini,et al.  Positive and negative-mode laser desorption/ionization-mass spectrometry (LDI-MS) for the detection of indigoids in archaeological purple. , 2013, Journal of mass spectrometry : JMS.

[7]  S. Kansa,et al.  Distinguishing between archaeological sheep and goat bones using a single collagen peptide , 2010 .

[8]  Š. Kučková,et al.  Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings. , 2004, Rapid communications in mass spectrometry : RCM.

[9]  Elmar Heinzle,et al.  Species identification of Oetzi's clothing with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based on peptide pattern similarities of hair digests. , 2008, Rapid communications in mass spectrometry : RCM.

[10]  Christian Rolando,et al.  Identification of proteins in renaissance paintings by proteomics. , 2006, Analytical chemistry.

[11]  J. Allison,et al.  Laser Desorption Mass Spectrometry as a Tool for the Analysis of Colorants: The Identification of Pigments Used in Illuminated Manuscripts* , 2004 .

[12]  Š. Kučková,et al.  Identification of proteinaceous binders used in artworks by MALDI-TOF mass spectrometry , 2007, Analytical and bioanalytical chemistry.

[13]  M. Buckley,et al.  Proteomics analysis of ancient food vessel stitching reveals >4000-year-old milk protein. , 2013, Rapid communications in mass spectrometry : RCM.

[14]  Applications of laser desorption mass spectrometry for the study of synthetic organic pigments in works of art , 2009 .

[15]  Julie Arslanoglu,et al.  Immunology and art: Using antibody-based techniques to identify proteins and gums in artworks , 2010, Journal of Biosciences.

[16]  J. Allison,et al.  Identification of Organic Pigments in Automotive Coatings Using Laser Desorption Mass Spectrometry * , 2007, Journal of forensic sciences.

[17]  M. Buckley,et al.  Collagen survival and its use for species identification in Holocene-lower Pleistocene bone fragment , 2011 .

[18]  J. Yates,et al.  An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database , 1994, Journal of the American Society for Mass Spectrometry.

[19]  Maria Perla Colombini,et al.  Deamidation at asparagine and glutamine as a major modification upon deterioration/aging of proteinaceous binders in mural paintings. , 2011, Analytical chemistry.

[20]  W. Fitzhugh,et al.  Identification of protein remains in archaeological potsherds by proteomics. , 2008, Analytical chemistry.

[21]  Mary W. Ballard,et al.  Proteomics and Coast Salish blankets: a tale of shaggy dogs? , 2011, Antiquity.

[22]  Christian Rolando,et al.  Identification of animal glue species in artworks using proteomics: application to a 18th century gilt sample. , 2011, Analytical chemistry.