Hepatitis B virus and Homo sapiens proteome-wide analysis: A profusion of viral peptide overlaps in neuron-specific human proteins

The primary amino acid sequence of the hepatitis B virus (HBV) proteome was searched for identity spots in the human proteome by using the Protein Information Resource database. We find that the HBV polyprotein shares sixty-five heptapeptides, one octapeptide, and one nonapeptide with the human proteins. The viral matches are disseminated among fundamental human proteins such as adhesion molecules, leukocyte differentiation antigens, enzymes, proteins associated with spermatogenesis, and transcription factors. As a datum of special interest, a number of peptide motifs are shared between the virus- and brain-specific antigens involved in neuronal protection. This study may help to evaluate the potential cross reactions and side effects of HBV antigen-based vaccines.

[1]  Klaus-Armin Nave,et al.  Shaping of the autoreactive T-cell repertoire by a splice variant of self protein expressed in thymic epithelial cells , 2000, Nature Medicine.

[2]  W. Hung,et al.  Multiple transcripts of the human Cu,Zn superoxide dismutase gene. , 2000, Biochemical and biophysical research communications.

[3]  Anne M. Bowcock,et al.  Identification of a RING protein that can interact in vivo with the BRCA1 gene product , 1996, Nature Genetics.

[4]  E. Sugikawa,et al.  Identification of a Novel Human Eicosanoid Receptor Coupled to Gi/o * , 2002, The Journal of Biological Chemistry.

[5]  W. Bickmore,et al.  Mammalian PRP4 Kinase Copurifies and Interacts with Components of Both the U5 snRNP and the N-CoR Deacetylase Complexes , 2002, Molecular and Cellular Biology.

[6]  C. Janeway.  Approaching the asymptote? Evolution and revolution in immunology. , 1989, Cold Spring Harbor symposia on quantitative biology.

[7]  A. Karlsson,et al.  Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases. , 2001, Molecular pharmacology.

[8]  M. Suyama,et al.  Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. , 1998, DNA research : an international journal for rapid publication of reports on genes and genomes.

[9]  Christopher J. Wilkinson,et al.  Rootletin forms centriole-associated filaments and functions in centrosome cohesion , 2005, The Journal of cell biology.

[10]  Carla Grandori,et al.  c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I , 2005, Nature Cell Biology.

[11]  D. Simmons,et al.  Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. , 1993, Blood.

[12]  T. Bonner,et al.  Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[13]  Y. Ilan,et al.  Beta-glycoglycosphingolipid-induced augmentation of the anti-HBV immune response is associated with altered CD8 and NKT lymphocyte distribution: a novel adjuvant for HBV vaccination. , 2008, Vaccine.

[14]  M. Brown,et al.  Improving vaccines by incorporating immunological coadjuvants , 2007, Expert review of vaccines.

[15]  Takeharu Hayashi,et al.  Titin mutations as the molecular basis for dilated cardiomyopathy. , 2002, Biochemical and biophysical research communications.

[16]  G. Blobel,et al.  The 210-kD nuclear envelope polypeptide recognized by human autoantibodies in primary biliary cirrhosis is the major glycoprotein of the nuclear pore. , 1990, The Journal of clinical investigation.

[17]  B. Trost,et al.  Peptidology: short amino acid modules in cell biology and immunology , 2007, Amino Acids.

[18]  J. Gottesfeld,et al.  Cyclin L Is an RS Domain Protein Involved in Pre-mRNA Splicing* , 2002, The Journal of Biological Chemistry.

[19]  S. Shirasawa,et al.  Deregulated expression of KRAP, a novel gene encoding actin-interacting protein, in human colon cancer cells , 2004, Journal of Human Genetics.

[20]  Mark Veugelers,et al.  Mutation of perinatal myosin heavy chain associated with a Carney complex variant. , 2004, The New England journal of medicine.

[21]  Ryan D. Morin,et al.  The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). , 2004, Genome research.

[22]  A. Kusalik,et al.  Massive peptide sharing between viral and human proteomes , 2008, Peptides.

[23]  M. Maines,et al.  Human biliverdin IXalpha reductase is a zinc-metalloprotein. Characterization of purified and Escherichia coli expressed enzymes. , 1996, European journal of biochemistry.

[24]  James L. Buescher,et al.  Tau‐tubulin kinase 1 (TTBK1), a neuron‐specific tau kinase candidate, is involved in tau phosphorylation and aggregation , 2006, Journal of neurochemistry.

[25]  T. Putilina,et al.  The DHHC domain: A new highly conserved cysteine-rich motif , 1999, Molecular and Cellular Biochemistry.

[26]  Jiahuai Han,et al.  Primary structure of BMK1: a new mammalian map kinase. , 1995, Biochemical and biophysical research communications.

[27]  O. Yoshie,et al.  Identification of Single C Motif-1/Lymphotactin Receptor XCR1* , 1998, The Journal of Biological Chemistry.

[28]  M. Lievens,et al.  Immunogenicity and safety of an adjuvanted hepatitis B vaccine in pre-hemodialysis and hemodialysis patients. , 2005, Kidney international.

[29]  Somasekar Seshagiri,et al.  The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. , 2003, Genome research.

[30]  C. Garner,et al.  The presynaptic cytomatrix protein Bassoon: sequence and chromosomal localization of the human BSN gene. , 1999, Genomics.

[31]  Christina A. Cuomo,et al.  Human chromosome 11 DNA sequence and analysis including novel gene identification , 2006, Nature.

[32]  T. Akazawa,et al.  TICAM-1, an adaptor molecule that participates in Toll-like receptor 3–mediated interferon-β induction , 2003, Nature Immunology.

[33]  D. Maślińska,et al.  Expression of IL‐15 and IL‐15 Receptor Isoforms in Select Structures of Human Fetal Brain , 2002, Annals of the New York Academy of Sciences.

[34]  D. Pham‐Dinh,et al.  Complex alternative splicing of the myelin oligodendrocyte glycoprotein gene is unique to human and non‐human primates , 2006, Journal of neurochemistry.

[35]  Andrew Menzies,et al.  Mutations in CUL4B, which encodes a ubiquitin E3 ligase subunit, cause an X-linked mental retardation syndrome associated with aggressive outbursts, seizures, relative macrocephaly, central obesity, hypogonadism, pes cavus, and tremor. , 2007, American journal of human genetics.

[36]  Roland M. Martin,et al.  Enhanced T cell responsiveness to citrulline-containing myelin basic protein in multiple sclerosis patients , 2000, Multiple sclerosis.

[37]  Gerhard Wagner,et al.  Structural studies of the interaction between ubiquitin family proteins and proteasome subunit S5a. , 2002, Biochemistry.

[38]  N. Nomura,et al.  Complete sequencing and characterization of 21,243 full-length human cDNAs , 2004, Nature Genetics.

[39]  S. Fröhling,et al.  hIan5: the human ortholog to the rat Ian4/Iddm1/lyp is a new member of the Ian family that is overexpressed in B-cell lymphoid malignancies , 2004, Genes and Immunity.

[40]  B. Olde,et al.  Identification of a free fatty acid receptor, FFA2R, expressed on leukocytes and activated by short-chain fatty acids. , 2003, Biochemical and biophysical research communications.

[41]  M. Oldstone.  Molecular mimicry and immune‐mediated diseases , 1998, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[42]  M. Kok,et al.  CHD5, a new member of the chromodomain gene family, is preferentially expressed in the nervous system , 2003, Oncogene.

[43]  I. Dunham,et al.  The DNA sequence and analysis of human chromosome 6 , 2003, Nature.

[44]  J. Bryan.  Developing an HPV vaccine to prevent cervical cancer and genital warts. , 2007, Vaccine.

[45]  Terrence S. Furey,et al.  The DNA sequence and biology of human chromosome 19 , 2004, Nature.

[46]  Uta Francke,et al.  The human neuregulin-2 (NRG2) gene: cloning, mapping and evaluation as a candidate for the autosomal recessive form of Charcot-Marie-Tooth disease linked to 5q , 1999, Human Genetics.

[47]  M. Colonna,et al.  Cloning of immunoglobulin-superfamily members associated with HLA-C and HLA-B recognition by human natural killer cells. , 1995, Science.

[48]  I. Dunham,et al.  DNA sequence and analysis of human chromosome 9 , 2003, Nature.

[49]  C. Florentz,et al.  Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS. , 2005, Biochemistry.

[50]  P. Sharp,et al.  A coactivator of pre-mRNA splicing. , 1998, Genes & development.

[51]  E. Nagata,et al.  PI3 kinase enhancer–Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis , 2003, Nature Neuroscience.

[52]  P. Leder,et al.  Structure and expression of the human immunoglobulin lambda genes , 1990, The Journal of experimental medicine.

[53]  L. A. McInnes,et al.  A quantitative regional expression profile of EAAT2 known and novel splice variants reopens the question of aberrant EAAT2 splicing in disease , 2007, Neurochemistry International.

[54]  M. Katoh,et al.  Comparative genomics on HHIP family orthologs. , 2006, International journal of molecular medicine.

[55]  G. Blobel,et al.  Primary structure of a human arginine-rich nuclear protein that colocalizes with spliceosome components. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[56]  Junjun Zhang,et al.  Human Chromosome 7: DNA Sequence and Biology , 2003, Science.

[57]  N. Sheikh,et al.  Smart adjuvants , 2007, Expert review of vaccines.

[58]  C. Power,et al.  Diminished adenosine A1 receptor expression on macrophages in brain and blood of patients with multiple sclerosis , 2001, Annals of neurology.

[59]  G. Matassi,et al.  Molecular characterization of a new urea transporter in the human kidney , 1996, FEBS letters.

[60]  Denis Hochstrasser,et al.  Functional proteomic analysis of human nucleolus. , 2002, Molecular biology of the cell.

[61]  W. Good,et al.  Suppressed expression of tubedown-1 in retinal neovascularization of proliferative diabetic retinopathy. , 2001, Investigative ophthalmology & visual science.

[62]  K. Pollard,et al.  Gold‐ and silver‐induced murine autoimmunity – requirement for cytokines and CD28 in murine heavy metal‐induced autoimmunity , 2009, Clinical and experimental immunology.

[63]  B. Seed,et al.  Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. , 1989, Science.

[64]  Tohru Natsume,et al.  Physical and Functional Interaction between Dorfin and Valosin-containing Protein That Are Colocalized in Ubiquitylated Inclusions in Neurodegenerative Disorders* , 2004, Journal of Biological Chemistry.

[65]  J. L. Rosa,et al.  The giant protein HERC1 is recruited to aluminum fluoride‐induced actin‐rich surface protrusions in HeLa cells , 2004, FEBS letters.

[66]  A. Hudspeth,et al.  RIM Binding Proteins (RBPs) Couple Rab3-Interacting Molecules (RIMs) to Voltage-Gated Ca2+ Channels , 2002, Neuron.

[67]  E. Sugikawa,et al.  Identification of a novel human eicosanoid receptor coupled to G(i/o). , 2002, The Journal of biological chemistry.