Amperometric biosensors based on an apparent direct electron transfer between electrodes and immobilized peroxidases. Plenary lecture

An apparent direct electron transfer between various electrode materials and peroxidases immobilized on the surface of the electrode has been reported in the last few years. An electrocatalytic reduction of hydrogen peroxide stars at about +600 mV versus a saturated calomel (reference) electrode (SCE) at neutral pH. The efficiency of the electrocatalytic current increases as the applied potential is made more negative and starts to level off at about –200 mV versus SCE. Amperometric biosensors for hydrogen peroxide can be constructed with these types of peroxidase modified electrodes. By co-immobilizing a hydrogen peroxide-producing oxidase with the peroxidase, amperometric biosensors can be made that respond to the substrate of the oxidase within a potential range essentially free of interfering electrochemical reactions. Examples of glucose, alcohol and amino acid sensors are shown.

[1]  Theodore Kuwana,et al.  Electrocatalysis of dihydronicotinamide adenosine diphosphate with quinones and modified quinone electrodes , 1978 .

[2]  L. Gorton,et al.  Liquid chromatographic separation and stereoselective detection of L- and D-amino acids with catalytic reaction detection using immobilized enzymes. , 1990, Journal of pharmaceutical and biomedical analysis.

[3]  Joseph Wang,et al.  Organic-phase enzymatic assays with ultramicroelectrodes , 1991 .

[4]  C. Malitesta,et al.  Glucose fast-response amperometric sensor based on glucose oxidase immobilized in an electropolymerized poly(o-phenylenediamine) film. , 1990, Analytical chemistry.

[5]  Masuo Aizawa,et al.  Principles and applications of electrochemical and optical biosensors , 1991 .

[6]  H. Shinohara,et al.  Enzyme microsensor for glucose with an electrochemically synthesized enzyme-polyaniline film , 1988 .

[7]  T. Tatsuma,et al.  Peroxidase model electrodes: heme peptide modified electrodes as reagentless sensors for hydrogen peroxide. , 1991, Analytical chemistry.

[8]  Lo Gorton,et al.  An amperometric glucose electrode based on adsorbed glucose oxidase on palladium/gold modified graphite , 1987 .

[9]  J. J. Kalys.  Enzyme electrodes based on organic metals , 1986 .

[10]  J. Kulys,et al.  Reagentless lactate electrodes based on electrocatalytic oxidation of flavocytochrome b2 , 1991 .

[11]  Björn Persson,et al.  Amperometric glucose sensors based on immobilized glucose-oxidizing enzymes and chemically modified electrodes , 1991 .

[12]  H. Hill,et al.  A method for estimation of hydrogen peroxide based on mediated electron transfer reactions of peroxidases at electrodes , 1986 .

[13]  Alexander M. Yacynych,et al.  Electropolymerized films to prevent interferences and electrode fouling in biosensors , 1991 .

[14]  Lo Gorton,et al.  An electrochemical sensor for hydrogen peroxide based on peroxidase adsorbed on a spectrographic graphite electrode , 1989 .

[15]  H. Dunford.  On the function and mechanism of action of peroxidases , 1976 .

[16]  L. Gorton,et al.  Enzymatic determination of glucose in a flow system by catalytic oxidation of the nicotinamide coenzyme at a modified electrode , 1985 .

[17]  L. Gorton,et al.  A glucose electrode based on carbon paste chemically modified with a ferrocene-containing siloxane polymer and glucose oxidase, coated with a poly(ester-sulfonic acid) cation-exchanger , 1990 .

[18]  M. Smyth,et al.  Flow-Injection Analysis of Hydrogen Peroxide Using a Horseradish Peroxidase-Modified Electrode Detection System , 1990 .

[19]  R G Whitaker,et al.  Strategies for the development of amperometric enzyme electrodes. , 1987, Biosensors.

[20]  E. F. Bowden,et al.  Electrocatalytic reduction of hydrogen peroxide via direct electron transfer from pyrolytic graphite electrodes to irreversibly adsorbed cytochrome c peroxidase , 1989 .

[21]  S. Ponrathnam,et al.  Polyethyleneimine in immobilization of biocatalysts. , 1991, Enzyme and microbial technology.

[22]  Alexander M. Yacynych,et al.  Electropolymerized 1,2-diaminobenzene as a means to prevent interferences and fouling and to stabilize immobilized enzyme in electrochemical biosensors , 1990 .

[23]  T. Yao,et al.  Flow injection analysis for glucose by the combined use of an immobilized glucose oxidase reactor and a peroxidase electrode , 1984 .

[24]  I. Yamazaki,et al.  The oxidation-reduction potentials of compound I/compound II and compound II/ferric couples of horseradish peroxidases A2 and C. , 1979, The Journal of biological chemistry.

[25]  F. Armstrong,et al.  Direct electrochemistry of redox proteins , 1988 .

[26]  G. Jönsson-Pettersson,et al.  Reagentless hydrogen peroxide and glucose sensors based on peroxidase immobilized on graphite electrodes , 1991 .

[27]  Björn Persson,et al.  Selective detection in flow analysis based on the combination of immobilized enzymes and chemically modified electrodes , 1991 .

[28]  U. Wollenberger,et al.  Bulk modified enzyme electrodes for reagentless detection of peroxides , 1991 .

[29]  G. Hicks,et al.  The Enzyme Electrode , 1967, Nature.

[30]  J. Kulys,et al.  401 — The development of bienzyme glucose electrodes , 1981 .

[31]  Frieder W. Scheller,et al.  Enzyme Electrodes Using Bioelectrocatalytic Reduction of Hydrogen Peroxide , 1990 .

[32]  K. Welinder.  Amino Acid Sequence Studies of Horseradish Peroxidase , 1979 .