Directional blood flow imaging in volumetric optical microangiography achieved by digital frequency modulation.

An effective digital frequency modulation approach to achieve directional blood flow imaging within microcirculations in tissue beds in vivo for optical microangiography is presented. The method only requires the system to capture one three-dimensional data set within which the interferograms are modulated by a constant frequency modulation that gives one directional flow information. The result is that the imaging speed is doubled and the computational load is halved. The method is experimentally validated by a flow phantom and is tested for imaging of cerebral vascular blood perfusion in a live mouse with the cranium left intact.

[1]  Ruikang K. Wang,et al.  Three-dimensional optical micro-angiography maps directional blood perfusion deep within microcirculation tissue beds in vivo , 2007, Physics in medicine and biology.

[2]  Teresa C. Chen,et al.  In vivo dynamic human retinal blood flow imaging using ultra-high-speed spectral domain optical Doppler tomography , 2003 .

[3]  Ruikang K. Wang.  In vivo full range complex Fourier domain optical coherence tomography , 2007 .

[4]  Ruikang K. Wang,et al.  Three dimensional optical angiography. , 2007, Optics express.

[5]  Ruikang K. Wang,et al.  Real-time flow imaging by removing texture pattern artifacts in spectral-domain optical Doppler tomography. , 2006, Optics letters.

[6]  G. Ha Usler,et al.  "Coherence radar" and "spectral radar"-new tools for dermatological diagnosis. , 1998, Journal of biomedical optics.

[7]  Ruikang K. Wang,et al.  Fourier domain optical coherence tomography achieves full range complex imaging in vivo by introducing a carrier frequency during scanning , 2007, Physics in medicine and biology.

[8]  M. V. van Gemert,et al.  Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography. , 1997, Optics letters.

[9]  Ruikang K. Wang,et al.  Mapping of cerebro-vascular blood perfusion in mice with skin and skull intact by Optical Micro-AngioGraphy at 1.3 mum wavelength. , 2007, Optics express.

[10]  Ruikang K. Wang,et al.  Use of a scanner to modulate spatial interferograms for in vivo full-range Fourier-domain optical coherence tomography. , 2007, Optics letters.