Serotyping, stx2 Subtyping, and Characterization of the Locus of Enterocyte Effacement Island of Shiga Toxin-Producing Escherichia coli and E. coli O157:H7 Strains Isolated from the Environment in France

ABSTRACT Twenty-seven Shiga toxin-producing Escherichia coli (STEC) strains were isolated from 207 stx-positive French environmental samples. Ten of these strains were positive for stx1, and 24 were positive for stx2 (10 were positive for stx2vh-a or stx2vh-b, 19 were positive for stx2d, and 15 were positive for stx2e). One strain belonged to serotype O157:H7, and the others belonged to serogroups O2, O8, O11, O26, O76, O103, O113, O121, O141, O166, and O174. The environment is a reservoir in which new clones of STEC that are pathogenic for humans can emerge.

[1]  V. Livrelli,et al.  Localization of the Insertion Site and Pathotype Determination of the Locus of Enterocyte Effacement of Shiga Toxin-Producing Escherichia coli Strains , 2004, Applied and Environmental Microbiology.

[2]  L. Beutin,et al.  Genetic Diversity of Intimin Genes of Attaching and Effacing Escherichia coli Strains , 2002, Journal of Clinical Microbiology.

[3]  C. Vernozy-Rozand,et al.  Prevalence of verotoxin‐producing Escherichia coli (VTEC) in slurry, farmyard manure and sewage sludge in France , 2002, Journal of applied microbiology.

[4]  C. Mazuy,et al.  Prevalence of verotoxin‐producing Escherichia coli (VTEC) and E. coli O157:H7 in French pork , 2002, Journal of applied microbiology.

[5]  T. Whittam,et al.  Molecular Evolution of the Intimin Gene in O111 Clones of Pathogenic Escherichia coli , 2002, Journal of bacteriology.

[6]  A. Friedrich,et al.  Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms. , 2002, The Journal of infectious diseases.

[7]  N. Pradel,et al.  Stx2 Subtyping of Shiga Toxin-Producing Escherichia coli Isolated from Cattle in France: Detection of a New Stx2 Subtype and Correlation with Additional Virulence Factors , 2001, Journal of Clinical Microbiology.

[8]  A. Maule.  Survival of verocytotoxigenic Escherichia coli O157 in soil, water and on surfaces , 2000, Symposium series.

[9]  F. Grimont,et al.  Identification of Escherichia coli flagellar types by restriction of the amplified fliC gene. , 2000, Research in microbiology.

[10]  H. Schmidt,et al.  Structural Analysis of Phage-Borne stx Genes and Their Flanking Sequences in Shiga Toxin-Producing Escherichia coli and Shigella dysenteriae Type 1 Strains , 2000, Infection and Immunity.

[11]  B. China,et al.  Comparison of eae, tir, espA and espB genes of bovine and human attaching and effacing Escherichia coli by multiplex polymerase chain reaction. , 1999, FEMS microbiology letters.

[12]  C. Höller,et al.  Isolation of enterohaemorrhagic Escherichia coli from municipal sewage , 1999, The Lancet.

[13]  B. China,et al.  Heterogeneity of the eae genes in attaching/effacing Escherichia coli from cattle: comparison with human strains. , 1999, Research in microbiology.

[14]  S. McEwen,et al.  Associations between Virulence Factors of Shiga Toxin-ProducingEscherichia coli and Disease in Humans , 1999, Journal of Clinical Microbiology.

[15]  L. Beutin,et al.  Human infections with Shiga toxin-producing Escherichia coli other than serogroup O157 in Germany. , 1998, Emerging infectious diseases.

[16]  V. Sperandio,et al.  Characterization of the locus of enterocyte effacement (LEE) in different enteropathogenic Escherichia coli (EPEC) and Shiga-toxin producing Escherichia coli (STEC) serotypes. , 1998, FEMS microbiology letters.

[17]  F. Grimont.  Recherche de Escherichia coli pathogènes dans les selles , 1998 .

[18]  J. Paton,et al.  Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. , 1998, Journal of clinical microbiology.

[19]  J. Bensink,et al.  Verotoxin-producing Escherichia coli from the faeces of sheep, calves and pigs. , 1997, Australian veterinary journal.

[20]  L. Beutin,et al.  Rapid detection and isolation of shiga-like toxin (verocytotoxin)-producing Escherichia coli by direct testing of individual enterohemolytic colonies from washed sheep blood agar plates in the VTEC-RPLA assay , 1996, Journal of clinical microbiology.

[21]  S. Grant,et al.  Prevalence of enterohemorrhagic Escherichia coli in raw and treated municipal sewage , 1996, Applied and environmental microbiology.

[22]  M. Doyle,et al.  Fate of enterohemorrhagic Escherichia coli O157:H7 in bovine feces , 1996, Applied and environmental microbiology.

[23]  Y. Tsai,et al.  Multiplex PCR for detection of the heat-labile toxin gene and shiga-like toxin I and II genes in Escherichia coli isolated from natural waters , 1994, Applied and environmental microbiology.

[24]  D. L. Clark,et al.  Detection of virulence factors in culturable Escherichia coli isolates from water samples by DNA probes and recovery of toxin-bearing strains in minimal o-nitrophenol-beta-D-galactopyranoside-4-methylumbelliferyl-beta-D-g luc uronide media , 1992, Applied and environmental microbiology.

[25]  M. P. Jackson,et al.  Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine , 1988, Journal of bacteriology.

[26]  I. Ørskov,et al.  2 Serotyping of Escherichia coli , 1984 .