Time-resolved polarization measurements of porphyrin fluorescence in solution and in single cells.

Porphyrin monomers, dimers and aggregates, which can be differentiated on the basis of their fluorescence lifetimes, are shown to possess different degrees of fluorescence polarization. This opens up new possibilities for microscopic imaging of these individual components in photosensitization and tumour detection. A rough estimate of the size of the porphyrin aggregates is obtained from the data of time-resolved fluorescence anisotropy.

[1]  H. Schneckenburger,et al.  Time-resolved fluorescence in photobiology. , 1988, Journal of photochemistry and photobiology. B, Biology.

[2]  H. Schneckenburger,et al.  INTRACELLULAR FLUORESCENCE OF PHOTOSENSITIZING PORPHYRINS AT DIFFERENT CONCENTRATIONS OF MITOCHONDRIA , 1988, Photochemistry and photobiology.

[3]  B. Thorell,et al.  MICROSPECTROFLUOROMETRIC APPROACH TO THE STUDY OF FREE/BOUND NAD(P)H RATIO AS METABOLIC INDICATOR IN VARIOUS CELL TYPES , 1982, Photochemistry and photobiology.

[4]  S. Gibson,et al.  INTERDEPENDENCE OF FLUENCE, DRUG DOSE and OXYGEN ON HEMATOPORPHYRIN DERIVATIVE INDUCED PHOTOSENSITIZATION OF TUMOR MITOCHONDRIA , 1985, Photochemistry and photobiology.

[5]  D. Kessel.  IN VIVO FLUORESCENCE OF TUMORS AFTER TREATMENT WITH DERIVATIVES OF HEMATOPORPHYRIN , 1986, Photochemistry and photobiology.

[6]  David Dolphin,et al.  Excitonic interactions in covalently-linked porphyrin dimers , 1981 .

[7]  R. Steiner,et al.  Intracellular distribution of photosensitizing porphyrins measured by video-enhanced fluorescence microscopy. , 1988, Journal of photochemistry and photobiology. B, Biology.

[8]  T J Dougherty,et al.  PHOTOSENSITIZERS: THERAPY AND DETECTION OF MALIGNANT TUMORS , 1987, Photochemistry and photobiology.

[9]  Rinaldo Cubeddu,et al.  Hematoporphyrin derivative: experimental evidence for aggregated species , 1982 .

[10]  I. Summerhayes,et al.  Decreased uptake and retention of rhodamine 123 by mitochondria in feline sarcoma virus-transformed mink cells , 1982, Cell.

[11]  M. Yamashita,et al.  Picosecond time-resolved fluorescence spectroscopy of hematoporphyrin derivative , 1984 .

[12]  R. Ramponi,et al.  QUANTITATIVE ANALYSIS OF INTRACELLULAR BEHAVIOUR OF PORPHYRINS , 1987, Photochemistry and photobiology.

[13]  G. Moreno,et al.  Photodynamic effects of haematoporphyrin on respiration and calcium uptake in isolated mitochondria. , 1981, International journal of radiation biology and related studies in physics, chemistry, and medicine.

[14]  J Moan,et al.  ACTION SPECTRA FOR HEMATOPORPHYRIN DERIVATIVE AND PHOTOFRIN II WITH RESPECT TO SENSITIZATION OF HUMAN CELLS IN VITRO TO PHOTOINACTIVATION , 1984, Photochemistry and photobiology.