Removal of nitrogen and phosphorus from agro-industrial wastewater by using microalgae collected from coastal region of peninsular Malaysia

© 2021 African Journal of Biological Sciences.This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Abstract The potential of microalgae as a source of renewable energy based on wastewater has received increasing interest worldwide in recent decades. A freshwater microalga Chlorella vulgaris was investigated for its ability to remove both nitrogen and phosphorus from three industrial wastewaters which were diluted in microalgae in two different proportions (namely, 50% and 75%). C. vulgaris grew fastest under 75% palm oil mill, and showed an maximum cell density (0.408 ± 0.012 g/L) for Palm Oil Mill Effluent (POME) wastewater, followed by riboflavin manufacturing wastewater (0.402 ± 0.083 g/L), and fertilizer industrial wastewater (0.320 ± 0.074 g/L), indicating the levels of nitrogen and phosphorus greatly influenced algal growth. Low removal efficiency for total nitrogen (TN) (11.35 ± 0.07% – 51.31 ± 0.03%) and total phosphorus (TP) (31.25 ± 0.24% – 93.62 ± 0.16%) was observed. C. vulgaris grew well when TP concentration was very low, indicating that this might be not the limiting factor to algal growth. The results suggest the potential of removing nutrient from wastewater by microalgae cultivation as production feedstock.

[1]  R. Ramaraj,et al.  Exploration of bioactive compounds and antibacterial activity of marine blue-green microalgae (Oscillatoria sp.) isolated from coastal region of west Malaysia , 2020, SN Applied Sciences.

[2]  A. Ramli,et al.  Antibacterial and antioxidative activity of the essential oil and seed extracts of Artocarpus heterophyllus for effective shelf‐life enhancement of stored meat , 2020, Journal of Food Processing and Preservation.

[3]  A. Ramli,et al.  Passion fruit (Passiflora edulis) peel powder extract and its application towards antibacterial and antioxidant activity on the preserved meat products , 2020, SN Applied Sciences.

[4]  V. Ponnusamy,et al.  The optimization of oil extraction from macroalgae, Rhizoclonium sp. by chemical methods for efficient conversion into biodiesel , 2020 .

[5]  M. Yusoff,et al.  EFFECT OF PLANT HORMONES ON THE PRODUCTION OF BIOMASS AND LIPID EXTRACTION FOR BIODIESEL PRODUCTION FROM MICROALGAE Chlorella Sp. , 2020 .

[6]  R. Ramaraj,et al.  Synthesis of silver nanoparticles using marine macroalgae Padina sp. and its antibacterial activity towards pathogenic bacteria , 2020, Beni-Suef University Journal of Basic and Applied Sciences.

[7]  E. Mandia,et al.  Desalination of Polymer and Chemical industrial wastewater by using green photosynthetic microalgae, Chlorella sp , 2020 .

[8]  R. Ramaraj,et al.  Microalgae cultivation using palm oil mill effluent as growth medium for lipid production with the effect of CO2 supply and light intensity , 2019 .

[9]  Q. Yuan,et al.  Removal of nitrogen from wastewater using microalgae and microalgae–bacteria consortia , 2016 .

[10]  Junhong Yang,et al.  Effects of Light and pH on Cell Density of Chlorella Vulgaris , 2014 .

[11]  J. Grobbelaar Inorganic Algal Nutrition , 2013 .

[12]  R. Andersen The Microalgal Cell , 2013 .

[13]  R. Prasanna,et al.  Evaluation of microalgal consortia for treatment of primary treated sewage effluent and biomass production , 2013, Journal of Applied Phycology.

[14]  Sa'id,et al.  Phytochemical, IR Spectral and Biological Studies on the Leaf Extracts of Commiphora Africana (Burseraceae) , 2013 .

[15]  S. Sriram,et al.  Microalgae Cultivation in Wastewater for Nutrient Removal , 2012 .

[16]  Y. Ghasemi,et al.  Effect of alginate structure and microalgae immobilization method on orthophosphate removal from wastewater , 2012, Journal of Applied Phycology.

[17]  Zhang Chunmin,et al.  Cultivation of Chlorella pyrenoidosa in soybean processing wastewater. , 2011, Bioresource technology.

[18]  G. Torzillo,et al.  Nitrogen and phosphorus removal through laboratory batch cultures of microalga Chlorella vulgaris and cyanobacterium Planktothrix isothrix grown as monoalgal and as co-cultures , 2011, Journal of Applied Phycology.

[19]  Paul Chen,et al.  Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production. , 2011, Bioresource technology.

[20]  Yujie Feng,et al.  Lipid production of Chlorella vulgaris cultured in artificial wastewater medium. , 2011, Bioresource technology.

[21]  Siew-Moi Phang,et al.  Use of Chlorella vulgaris for bioremediation of textile wastewater. , 2010, Bioresource technology.

[22]  K. C. Das,et al.  Biomass and bioenergy production potential of microalgae consortium in open and closed bioreactors using untreated carpet industry effluent as growth medium. , 2010, Bioresource technology.

[23]  C. Heim,et al.  Algae Production in Wastewater Treatment: Prospects for Ballen , 2010 .

[24]  T. Stephenson,et al.  Growth and nutrient removal in free and immobilized green algae in batch and semi-continuous cultures treating real wastewater. , 2010, Bioresource technology.

[25]  I. Kapdan,et al.  Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae , 2006 .

[26]  Yutaka Dote,et al.  Growth of the hydrocarbon-rich microalga Botryococcus braunii in secondarily treated sewage , 1992, Applied Microbiology and Biotechnology.

[27]  G. Kleinheinz,et al.  Use of Chlorella vulgaris for CO2 mitigation in a photobioreactor , 2002, Journal of Industrial Microbiology and Biotechnology.

[28]  Y. Bashan,et al.  Treatment of recalcitrant wastewater from ethanol and citric acid production using the microalga Chlorella vulgaris and the macrophyte Lemna minuscula. , 2002, Water research.

[29]  Yeoung-Sang Yun,et al.  Carbon Dioxide Fixation by Algal Cultivation Using Wastewater Nutrients , 1997 .

[30]  S. Baena,et al.  Efficiency of ammonia and phosphorus removal from a colombian agroindustrial wastewater by the microalgae Chlorella vulgaris and Scenedesmus dimorphus , 1997 .

[31]  Yuk Shan Wong,et al.  Wastewater Nutrients Removal by Chlorella Vulgaris: Optimization Through Acclimation , 1996 .

[32]  Claude E. Boyd,et al.  Orthophosphate uptake by phytoplankton and sediment , 1981 .

[33]  G. Cohen-bazire,et al.  Purification and properties of unicellular blue-green algae (order Chroococcales). , 1971, Bacteriological reviews.

[34]  M. Yusoff,et al.  A selective microalgae strain for biodiesel production in relation to higher lipid profile , 2022 .