Transcription inhibition using oligonucleotide-modified gold nanoparticles.

The capture of T7 RNA polymerase using double-stranded promoter DNA on the surface of gold nanoparticles has been demonstrated. The competitive binding and inhibition of T7 RNA polymerase due to specific interactions on the nanoparticle surface represents a transcription factor decoy approach in a model system. The efficiency of inhibition was determined for various nanoparticle sizes, surface coverage, and linker length for double-stranded promoter DNA on gold nanoparticles. The experiments provide a basis for determining the accessibility of binding sites on nanoparticle surfaces for applications involving cell targeting or the use of nanoparticles as binding agents in solution.

[1]  Chad A Mirkin,et al.  A thermodynamic investigation into the binding properties of DNA functionalized gold nanoparticle probes and molecular fluorophore probes. , 2005, Journal of the American Chemical Society.

[2]  J. Messing,et al.  A new opaque variant of maize by a single dominant RNA-interference-inducing transgene. , 2003, Genetics.

[3]  R. Levine,et al.  New Efficient Catheter-Based System for Myocardial Gene Delivery , 2002, Circulation.

[4]  Y. Cho‐Chung.  Antisense and therapeutic oligonucleotides: toward a gene-targeting cancer clinic , 2000 .

[5]  M. Gleave,et al.  A novel antisense oligonucleotide inhibiting several antiapoptotic Bcl-2 family members induces apoptosis and enhances chemosensitivity in androgen-independent human prostate cancer PC3 cells , 2005, Molecular Cancer Therapeutics.

[6]  M. Mann,et al.  Therapeutic applications of transcription factor decoy oligonucleotides. , 2000, The Journal of clinical investigation.

[7]  S. Franzen,et al.  Multifunctional gold nanoparticle-peptide complexes for nuclear targeting. , 2003, Journal of the American Chemical Society.

[8]  D. Scadden,et al.  Ex vivo targeting of p21Cip1/Waf1 permits relative expansion of human hematopoietic stem cells. , 2003, Blood.

[9]  E. Pearce,et al.  Transgene expression in Schistosoma mansoni: introduction of RNA into schistosomula by electroporation. , 2004, Molecular and biochemical parasitology.

[10]  Glenn P. Goodrich,et al.  Hybridization and enzymatic extension of au nanoparticle-bound oligonucleotides. , 2002, Journal of the American Chemical Society.

[11]  C. Mirkin,et al.  A two-color-change, nanoparticle-based method for DNA detection. , 2005, Talanta.

[12]  Chad A. Mirkin,et al.  One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle Probes , 1998 .

[13]  R. Morishita,et al.  Antisense oligonucleotides as a powerful molecular strategy for gene therapy in cardiovascular diseases. , 2004, Current pharmaceutical design.

[14]  S. Woo,et al.  Downregulation of human type III collagen gene expression by antisense oligodeoxynucleotide. , 2005, Tissue engineering.

[15]  J. Grandis,et al.  In vivo antitumor efficacy of STAT3 blockade using a transcription factor decoy approach: implications for cancer therapy , 2005, Oncogene.

[16]  Federica Gemignani,et al.  Systemically delivered antisense oligomers upregulate gene expression in mouse tissues , 2002, Nature Biotechnology.

[17]  A. Sinskey,et al.  Manipulating gene expression for the metabolic engineering of plants. , 2002, Metabolic engineering.

[18]  M. K. Brennaman,et al.  Critical flocculation concentrations, binding isotherms, and ligand exchange properties of peptide-modified gold nanoparticles studied by UV-visible, fluorescence, and time-correlated single photon counting spectroscopies. , 2003, Analytical chemistry.

[19]  Yanli Liu,et al.  Cellular trajectories of peptide-modified gold particle complexes: comparison of nuclear localization signals and peptide transduction domains. , 2004, Bioconjugate chemistry.

[20]  Young-Chae Chang,et al.  E2F decoy oligodeoxynucleotides effectively inhibit growth of human tumor cells. , 2003, Biochemical and biophysical research communications.

[21]  Y. Inagaki,et al.  Interferon-γ Interferes with Transforming Growth Factor-β Signaling through Direct Interaction of YB-1 with Smad3* , 2003, Journal of Biological Chemistry.

[22]  A. Steel,et al.  Electrochemical quantitation of DNA immobilized on gold. , 1998, Analytical chemistry.