Incorporating Graphene Oxide and Gold Nanoclusters: A Synergistic Catalyst with Surprisingly High Peroxidase‐Like Activity Over a Broad pH Range and its Application for Cancer Cell Detection

A synergistic graphene oxide-gold nanocluster (GO-AuNC) hybrid has been constructed as an enzyme mimic that is able to show high catalytic activity over a broad pH range, especially at neutral pH. Importantly, the target-functionalized hybrid has been applied as a robust nanoprobe for selective, quantitative, and fast colorimetric detection of cancer cells.

[1]  Z. Rahman,et al.  Graphene oxide-Fe3O4 magnetic nanocomposites with peroxidase-like activity for colorimetric detection of glucose. , 2012, Nanoscale.

[2]  Chunhai Fan,et al.  Catalytic gold nanoparticles for nanoplasmonic detection of DNA hybridization. , 2011, Angewandte Chemie.

[3]  R. Arakawa,et al.  ph‐Dependent Synthesis of Pepsin‐Mediated Gold Nanoclusters with Blue Green and Red Fluorescent Emission , 2011 .

[4]  Tom Regier,et al.  Co₃O₄ nanocrystals on graphene as a synergistic catalyst for oxygen reduction reaction. , 2011, Nature materials.

[5]  Miqin Zhang,et al.  Methotrexate-modified superparamagnetic nanoparticles and their intracellular uptake into human cancer cells. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[6]  Ding Ma,et al.  Caged-protein-confined bimetallic structural assemblies with mimetic peroxidase activity. , 2012, Small.

[7]  Xiaogang Qu,et al.  Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and Its Application to Glucose Detection , 2010, Advanced materials.

[8]  Shouwu Guo,et al.  Folic Acid-conjugated Graphene Oxide loaded with Photosensitizers for Targeting Photodynamic Therapy , 2011, Theranostics.

[9]  J. Ferlay,et al.  Global Cancer Statistics, 2002 , 2005, CA: a cancer journal for clinicians.

[10]  Per Berglund,et al.  Engineered enzymes for improved organic synthesis. , 2003, Current opinion in biotechnology.

[11]  R. Dickson,et al.  Shuttle-based fluorogenic silver-cluster biolabels. , 2009, Angewandte Chemie.

[12]  Xiaobin Fan,et al.  Graphene supported Au-Pd bimetallic nanoparticles with core-shell structures and superior peroxidase-like activities , 2011 .

[13]  Shuai Hou,et al.  Design of AgM Bimetallic Alloy Nanostructures (M = Au, Pd, Pt) with Tunable Morphology and Peroxidase-Like Activity , 2010 .

[14]  E. Yeung,et al.  A handheld device for potential point-of-care screening of cancer. , 2007, The Analyst.

[15]  A. Antony,et al.  The biological chemistry of folate receptors. , 1992, Blood.

[16]  Shusheng Zhang,et al.  High-intensity fluorescence imaging and sensitive electrochemical detection of cancer cells by using an extracellular supramolecular reticular DNA-quantum dot sheath. , 2011, Chemistry.

[17]  Guoliang Zhang,et al.  Palladium nanoparticle-graphene hybrids as active catalysts for the Suzuki reaction , 2010 .

[18]  X. Qu,et al.  Selective and quantitative cancer cell detection using target-directed functionalized graphene and its synergetic peroxidase-like activity. , 2011, Chemical communications.

[19]  Kai Yang,et al.  Functionalized graphene oxide in enzyme engineering: a selective modulator for enzyme activity and thermostability. , 2012, ACS nano.

[20]  Bing Xu,et al.  A supramolecular-hydrogel-encapsulated hemin as an artificial enzyme to mimic peroxidase. , 2007, Angewandte Chemie.

[21]  Jianping Xie,et al.  Protein-directed synthesis of highly fluorescent gold nanoclusters. , 2009, Journal of the American Chemical Society.

[22]  H. Frey,et al.  Water‐Soluble Fluorescent Ag Nanoclusters Obtained from Multiarm Star Poly(acrylic acid) as “Molecular Hydrogel” Templates , 2007 .

[23]  Yi Lu,et al.  Lysozyme-stabilized gold fluorescent cluster: Synthesis and application as Hg(2+) sensor. , 2010, The Analyst.

[24]  H. Dunford,et al.  Mechanism of the oxidation of 3,5,3',5'-tetramethylbenzidine by myeloperoxidase determined by transient- and steady-state kinetics. , 1997, Biochemistry.

[25]  W. Foulkes,et al.  Folate-binding protein is a marker for ovarian cancer. , 1991, Cancer research.

[26]  K. Teker.  Bioconjugated carbon nanotubes for targeting cancer biomarkers , 2008 .

[27]  M. Karplus,et al.  How Enzymes Work: Analysis by Modern Rate Theory and Computer Simulations , 2004, Science.

[28]  Zhi Shan,et al.  BSA-stabilized Au clusters as peroxidase mimetics for use in xanthine detection. , 2011, Biosensors & bioelectronics.

[29]  Xiaogang Qu,et al.  Visual and quantitative detection of copper ions using magnetic silica nanoparticles clicked on multiwalled carbon nanotubes. , 2010, Chemical communications.

[30]  M. Liu,et al.  Stimuli-responsive peroxidase mimicking at a smart graphene interface. , 2012, Chemical communications.

[31]  J. Barber.  Photosynthetic energy conversion: natural and artificial. , 2009, Chemical Society reviews.

[32]  Y. Liu,et al.  Au@Pt nanostructures as oxidase and peroxidase mimetics for use in immunoassays. , 2011, Biomaterials.

[33]  Hui Jiang,et al.  Gold nanoclusters and graphene nanocomposites for drug delivery and imaging of cancer cells. , 2011, Angewandte Chemie.

[34]  Yanglong Hou,et al.  Liquid-phase exfoliation, functionalization and applications of graphene. , 2011, Nanoscale.

[35]  Xiaochun Wu,et al.  Screening of inhibitors for oxidase mimics of Au@Pt nanorods by catalytic oxidation of OPD. , 2011, Chemical communications.

[36]  V. Dravid,et al.  Graphene oxide as an enzyme inhibitor: modulation of activity of α-chymotrypsin. , 2011, Journal of the American Chemical Society.

[37]  Jing Li,et al.  Hemin-graphene hybrid nanosheets with intrinsic peroxidase-like activity for label-free colorimetric detection of single-nucleotide polymorphism. , 2011, ACS nano.

[38]  Itaru Honma,et al.  Enhanced electrocatalytic activity of Pt subnanoclusters on graphene nanosheet surface. , 2009, Nano letters.

[39]  Rui Cao,et al.  Positively-charged gold nanoparticles as peroxidase mimic and their application in hydrogen peroxide and glucose detection. , 2010, Chemical communications.

[40]  Zhiyong Tang,et al.  Facile synthesis of surfactant-free Au cluster/graphene hybrids for high-performance oxygen reduction reaction. , 2012, ACS nano.

[41]  P. Paterlini-Bréchot,et al.  Circulating tumor cells (CTC) detection: clinical impact and future directions. , 2007, Cancer letters.

[42]  X. Duan,et al.  Graphene-supported hemin as a highly active biomimetic oxidation catalyst. , 2012, Angewandte Chemie.

[43]  Guillermo C Bazan,et al.  DNA hybridization detection with water-soluble conjugated polymers and chromophore-labeled single-stranded DNA. , 2003, Journal of the American Chemical Society.

[44]  W. Tseng,et al.  Ultrasensitive sensing of Hg(2+) and CH(3)Hg(+) based on the fluorescence quenching of lysozyme type VI-stabilized gold nanoclusters. , 2010, Analytical chemistry.

[45]  Zhijun Zhang,et al.  Functional graphene oxide as a nanocarrier for controlled loading and targeted delivery of mixed anticancer drugs. , 2010, Small.

[46]  Yu Zhang,et al.  Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. , 2007, Nature nanotechnology.

[47]  Hongtao Yu,et al.  Interface engineering catalytic graphene for smart colorimetric biosensing. , 2012, ACS nano.