Preliminary Identification of Sugarcane Mosaic Pathogeny in Yunnan

Preliminary identification of sugarcane mosaic pathogeny was undertaken in Yunnan sugarcane planting regions, and the disease pathogeny infection occurred on complexity infecting by the simple linear or spherical virus or by the complex both of them: Lots of linear and crooked virus particles in 750nm length can be seen under the electron microscope in the sample containing linear virus, which had the same characters as Potyviridae virus: plentiful wheel-like and tome-like inclusion body in the cytoplasm; 17 samples were examined by serology, and there were 10 samples checked by antibody as a positive reaction, Carried out RT-PCR on 5 samples based on the special characters of Potyviridae virus primers, the amplification product was according with the anticipation, which made clear that the virus is a kind of Potyvirus virus; The other 7 samples were checked by antibody as a negative reaction, which proved that the virus carried with the Potyviridae virus and not to belong the Potyvirus virus. The virus on 13 sugarcane varieties or kindred section varieties from 5 samples collected universally, had evident differences in infection ability, the No.11 infected 10 sugarcane varieties, was the most serious and with the most extensively distribution.