Entomopathogenic fungus, Lecanicillium lecanii R. Z are & W. Gams anchored into MCM-41: A new and effective bio-insecticide against Brevicoryne brassicae (Linnaeus, 1758) (Hom: Aphididae) to protect cabbages

Brevicoryne brassicae is a significant pest of cultivated cabbages and vegetable crops in the world. The present study was carried out to examine a potential strategy to enhance the insecticidal activity of Lecanicillium lecanii for cost-effective management of B. brassicae. The insecticidal efficacy of pure entomopathogenic fungus (PEF) and MCM-41 (Mobil Composition of Matter) L. lecanii were assessed against the cabbage aphid under laboratory and greenhouse conditions. The fungus was supported on MCM-41 and was completely characterized by Scanning Electron Microscope (SEM), thermogravimetric analysis (TGA) and Fourier transform infrared (FT-IR) techniques. LC50 values of PEF and MCM-41@fungus were 1.9×106 and 2.5×104 and 2.0×107 and 2.0×105 conidia/ml on adults of B. brassicae under laboratory and greenhouse conditions, respectively. Bioassays demonstrated that MCM-41@fungus significantly decreased LC50 values of entomopathogenic fungus and it was more toxic than L. lecanii at adult stage of the pest. The results showed that pure L. lecanii and its nano-formulation could play key roles as bio-pesticides in B. brassicae management programs.

[1]  B. Ramanujam,et al.  Field evaluation of entomopathogenic fungi against cabbage aphid, Brevicoryne brassicae (L.) and their effect on coccinellid predator , Coccinella septempunctata (Linnaeus) , 2018 .

[2]  S. Kamali,et al.  Biocontrol of Planococcus citri (Hemiptera: Pseudococcidae) by Lecanicillium longisporum and Lecanicillium lecanii under laboratory and greenhouse conditions , 2017 .

[3]  T. Alavo.  The insect pathogenic fungus Verticillium lecanii (Zimm.) Viegas and its use for pests control: a review. , 2015 .

[4]  A. Ghorbani‐Choghamarani,et al.  Bidentate salen Cu(II) complex functionalized on mesoporous MCM-41 as novel nano catalyst for the oxidative coupling of thiols into disulfides using urea hydrogen peroxide (UHP) , 2015, Journal of Porous Materials.

[5]  M. Sabbour.  Evaluating toxicity of extracted nano -Destruxin against the desert locust Schistocerca gregaria in Egypt , 2014 .

[6]  Matsunami,et al.  Biological Activity , 2014 .

[7]  Gamal A. M. AbduAllah.  Aphicidal activity of Imidacloprid and Primicarb compared with certain plant extracts on Brevicoryne brassicae L. and Aphis craccivora Koch , 2012, Assiut Journal of Agricultural Sciences.

[8]  M. Abdollahi-Alibeik,et al.  Nanosized MCM-41 supported protic ionic liquid as an efficient novel catalytic system for Friedlander synthesis of quinolines , 2012 .

[9]  K. Parida,et al.  Copper and Nickel Modified MCM-41 An Efficient Catalyst for Hydrodehalogenation of Chlorobenzene at Room Temperature , 2011 .

[10]  R. Gupta,et al.  Biological activity of some plant extracts against Pieris brassicae (Linn.) , 2009 .

[11]  M. Goettel,et al.  Potential of Lecanicillium spp. for management of insects, nematodes and plant diseases. , 2008, Journal of invertebrate pathology.

[12]  S. Shylesh,et al.  Vanadium-containing ordered mesoporous silicates: Does the silica source really affect the catalytic activity, structural stability, and nature of vanadium sites in V-MCM-41? , 2005 .

[13]  Qiang Cai,et al.  Dilute solution routes to various controllable morphologies of MCM-41 silica with a basic medium , 2001 .

[14]  F. P. Neupane.  Field Evaluation of Botanicals for the Management of Cruciferous Vegetable Insect Pests , 1999 .

[15]  Á. Lagunes-Tejeda,et al.  Susceptibility of Phthorimaea operculella (Zeller) to Insecticides , 1996 .

[16]  A. Beckett,et al.  Invasion and development of Verticillium lecanii in the western flower thrips, Frankliniella occidentalis , 1994 .

[17]  K. Raman,et al.  Effect of six synthetic pyrethroids on two populations of potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), in Peru , 1986 .

[18]  R. Hall.  fungus Verticillium lecanii as a microbial insecticide against aphids and scales , 1981 .

[19]  H. D. Burges.  Microbial control of pests and plant diseases 1970-1980 , 1981 .

[20]  M. Kanaoka,et al.  Bassianolide, a New Insecticidal Cyclodepsipeptide from Beauveria bassiana and Verticillium lecanii , 1977 .