Multi-stage analysis of gene expression and transcription regulation in C57/B6 mouse liver development.

The liver performs a number of essential functions for life. The development of such a complex organ relies on finely regulated gene expression profiles which change over time in the development and determine the phenotype and function of the liver. We used high-density oligonucleotide microarrays to study the gene expression and transcription regulation at 14 time points across the C57/B6 mouse liver development, which include E11.5 (embryonic day 11.5), E12.5, E13.5, E14.5, E15.5, E16.5, E17.5, E18.5, Day0 (the day of birth), Day3, Day7, Day14, Day21, and normal adult liver. With these data, we made a comprehensive analysis on gene expression patterns, functional preferences and transcriptional regulations during the liver development. A group of uncharacterized genes which might be involved in the fetal hematopoiesis were detected.

[1]  D. Botstein,et al.  Cluster analysis and display of genome-wide expression patterns. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[2]  S. Thorgeirsson,et al.  A novel prognostic subtype of human hepatocellular carcinoma derived from hepatic progenitor cells , 2006, Nature Medicine.

[3]  Michael Q. Zhang,et al.  DNA motifs in human and mouse proximal promoters predict tissue-specific expression. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[4]  P. Delost,et al.  Mortality in premature mice at birth and during neonatal development. , 1983, Reproduction, nutrition, developpement.

[5]  Xuesong Lu,et al.  The effect of GeneChip gene definitions on the microarray study of cancers. , 2006, BioEssays : news and reviews in molecular, cellular and developmental biology.

[6]  H. Nishina,et al.  Liver development and regeneration: From laboratory study to clinical therapy , 2007, Development, growth & differentiation.

[7]  J. R. Coleman,et al.  Hepatic specification of the gut endoderm in vitro: cell signaling and transcriptional control. , 1996, Genes & development.

[8]  Rafael A Irizarry,et al.  Exploration, normalization, and summaries of high density oligonucleotide array probe level data. , 2003, Biostatistics.

[9]  A. Zorn,et al.  Molecular control of liver development. , 2006, Clinics in liver disease.

[10]  Rodger Staden,et al.  Methods for calculating the probabilities of finding patterns in sequences , 1989, Comput. Appl. Biosci..

[11]  R. Myers,et al.  Evolving gene/transcript definitions significantly alter the interpretation of GeneChip data , 2005, Nucleic acids research.