Surfactant protein-A binds group B streptococcus enhancing phagocytosis and clearance from lungs of surfactant protein-A-deficient mice.

Surfactant protein-A (SP-A) gene-targeted mice clear group B streptococcus (GBS) from the lungs at a slower rate than wild-type mice. To determine mechanisms by which SP-A enhances pulmonary clearance of GBS, the role of SP-A in binding and phagocytosis of GBS was assessed in SP-A (-/-) mice infected with GBS in the presence and absence of exogenous SP-A. Coadministration of GBS with exogenous SP-A decreased GBS colony counts in lung homogenates of SP-A (-/-) mice. SP-A bound to GBS in a calcium-dependent manner. Although pulmonary infiltration with macrophages was not altered in SP-A (-/-) versus wild-type mice after GBS infection, the number of alveolar macrophages with phagocytosed bacteria was lower in the SP-A (-/-) mice than in the wild-type mice. When SP-A was coadministered with GBS, phagocytosis was significantly increased. Oxygen radical production by alveolar macrophages from SP-A (-/-) mice infected with GBS was decreased compared with wild-type controls and was increased when SP-A (-/-) mice were infected in the presence of exogenous SP-A. Superoxide (SO) radical generation was deficient in macrophages from SP-A (-/-) mice. SP-A plays an important role in GBS clearance in vivo, mediated in part by binding to and enhancing GBS phagocytosis and by increasing SO production by alveolar macrophages.

[1]  A. Jobe,et al.  Characteristics of surfactant from SP-A-deficient mice. , 1998, American journal of physiology. Lung cellular and molecular physiology.

[2]  A. Jobe,et al.  Effects of ventilation style on surfactant metabolism and treatment response in preterm lambs. , 1998, American journal of respiratory and critical care medicine.

[3]  W. Martin,et al.  Surfactant protein A (SP-A) mediates attachment of Mycobacterium tuberculosis to murine alveolar macrophages. , 1997, American journal of respiratory cell and molecular biology.

[4]  J. Whitsett,et al.  Surfactant protein A-deficient mice are susceptible to group B streptococcal infection. , 1997, Journal of immunology.

[5]  I. Ofek,et al.  SP-A enhances phagocytosis of Klebsiella by interaction with capsular polysaccharides and alveolar macrophages. , 1997, The American journal of physiology.

[6]  A. Lucas,et al.  Paediatric nutrition as a new subspecialty: is the time right? , 1997, Archives of disease in childhood.

[7]  C. Jarstrand,et al.  Surfactant improves lung function and mitigates bacterial growth in immature ventilated rabbits with experimentally induced neonatal group B streptococcal pneumonia , 1997, Archives of disease in childhood. Fetal and neonatal edition.

[8]  B. Stripp,et al.  Altered surfactant function and structure in SP-A gene targeted mice. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[9]  M. Pollack,et al.  Surfactant content in children with inflammatory lung disease. , 1996, Critical care medicine.

[10]  M. Sherman,et al.  Effect of different surfactants on pulmonary group B streptococcal infection in premature rabbits. , 1994, The Journal of pediatrics.

[11]  T. Schaberg,et al.  Surfactant protein A modulates release of reactive oxygen species from alveolar macrophages. , 1994, The American journal of physiology.

[12]  C. Jarstrand,et al.  Experimental Neonatal Group B Streptococcal Pneumonia: Effect of a Modified Porcine Surfactant on Bacterial Proliferation in Ventilated Near-Term Rabbits , 1994, Pediatric Research.

[13]  M. Daha,et al.  Binding of surfactant protein A to C1q receptors mediates phagocytosis of Staphylococcus aureus by monocytes. , 1994, The American journal of physiology.

[14]  J. Coonrod,et al.  Aggregation and opsonization of type A but not type B Hemophilus influenzae by surfactant protein A. , 1994, American journal of respiratory cell and molecular biology.

[15]  J. Wright,et al.  Pulmonary surfactant protein A stimulates chemotaxis of alveolar macrophage. , 1993, The American journal of physiology.

[16]  J. Whitsett,et al.  Decreased surfactant protein A in patients with bacterial pneumonia. , 1993, The American review of respiratory disease.

[17]  R. Ezekowitz,et al.  Collectins: pattern recognition molecules involved in first line host defense. , 1993, Current opinion in immunology.

[18]  Coonrod Jd,et al.  Comparison of the Opsonic Activity of Human Surfactant Protein A for Staphylococcus aureus and Streptococcus pneumoniae with Rabbit and Human Macrophages , 1993 .

[19]  J. Coonrod,et al.  Comparison of the opsonic activity of human surfactant protein A for Staphylococcus aureus and Streptococcus pneumoniae with rabbit and human macrophages. , 1993, The Journal of infectious diseases.

[20]  E. Crouch,et al.  Interactions of surfactant protein D with bacterial lipopolysaccharides. Surfactant protein D is an Escherichia coli-binding protein in bronchoalveolar lavage. , 1992, The Journal of clinical investigation.

[21]  J. Wright,et al.  Specific binding of surfactant apoprotein SP-A to rat alveolar macrophages. , 1992, The American journal of physiology.

[22]  H. Plattner,et al.  Lung surfactant protein A (SP-A) enhances serum-independent phagocytosis of bacteria by alveolar macrophages. , 1992, European journal of cell biology.

[23]  K. Maier,et al.  Effect of canine surfactant protein (SP‐A) on the respiratory burst of phagocytic cells , 1990, FEBS letters.

[24]  S. Thiel,et al.  Human leukocyte C1q receptor binds other soluble proteins with collagen domains , 1990, The Journal of experimental medicine.

[25]  R. Dessau,et al.  In Vivo and In Vitro Antibacterial Activity of Conglutinin, a Mammalian Plasma Lectin , 1990, Scandinavian journal of immunology.

[26]  J. Whitsett,et al.  Surfactant protein A concentrations in tracheal aspirate fluid from infants requiring extracorporeal membrane oxygenation. , 1990, The Journal of pediatrics.

[27]  J. Verhoef,et al.  Pulmonary surfactant protein A enhances the host-defense mechanism of rat alveolar macrophages. , 1990, American journal of respiratory cell and molecular biology.

[28]  W. Bullock,et al.  Phagocytosis of Histoplasma capsulatum yeasts and microconidia by human cultured macrophages and alveolar macrophages. Cellular cytoskeleton requirement for attachment and ingestion. , 1990, The Journal of clinical investigation.

[29]  S. Thiel,et al.  ASSOCIATION OF LOW LEVELS OF MANNAN-BINDING PROTEIN WITH A COMMON DEFECT OF OPSONISATION , 1989, The Lancet.

[30]  J. Wright,et al.  Lung surfactant apoprotein SP-A (26-36 kDa) binds with high affinity to isolated alveolar type II cells. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[31]  Kenneth Reid,et al.  Structures and functions associated with the group of mammalian lectins containing collagen‐like sequences , 1989, FEBS letters.

[32]  J. Groopman,et al.  A human serum mannose-binding protein inhibits in vitro infection by the human immunodeficiency virus , 1989, The Journal of experimental medicine.

[33]  W. Busse,et al.  Stimulus-dependent differences in superoxide anion generation by normal human eosinophils and neutrophils. , 1988, The Journal of allergy and clinical immunology.

[34]  K. Drickamer,et al.  The major lung surfactant protein, SP 28-36, is a calcium-dependent, carbohydrate-binding protein. , 1987, The Journal of biological chemistry.

[35]  J. Whitsett,et al.  Surfactant Associated Protein (SAP‐35) in Amniotic Fluid FroMDiabetic and Nondiabetic Pregnancies , 1987, Obstetrics and gynecology.

[36]  D. Nielson.  Electrolyte composition of pulmonary alveolar subphase in anesthetized rabbits. , 1986, Journal of applied physiology.

[37]  R. Wennberg,et al.  Group B Streptococcal Lung Infection in Neonatal Rabbits , 1982, Pediatric Research.

[38]  E. Goldstein,et al.  Murine pulmonary alveolar macrophages: rates of bacterial ingestion, inactivation, and destruction. , 1976, The Journal of infectious diseases.