The impact of nitrogen starvation on the dynamics of triacylglycerol accumulation in nine microalgae strains.

Microalgae-derived lipids are an alternative to vegetable and fossil oils, but lipid content and quality vary among microalgae strains. Selection of a suitable strain for lipid production is therefore of paramount importance. Based on published results for 96 species, nine strains were selected to study their biomass, total fatty acid, and triacylglycerol (TAG) production under nitrogen-sufficient and deficient cultivation conditions. Under nitrogen-deficient conditions, Chlorella vulgaris, Chlorella zofingiensis, Neochloris oleoabundans, and Scenedesmus obliquus, accumulated more than 35% of their dry weight as TAGs. Palmitic and oleic acid were the major fatty acids produced. The main difference between these strains was the amount of biomass that was produced (3.0-7.8-fold increase in dry weight) and the duration that the biomass productivity was retained (2-7 days) after nitrogen depletion. S. obliquus (UTEX 393) and C. zofingiensis (UTEX B32) showed the highest average TAG productivity (322 and 243 mg l(-1)day(-1)).

[1]  M. Eppink,et al.  Microalgae for the production of bulk chemicals and biofuels , 2010 .

[2]  Rocky de Nys,et al.  Growth, lipid content, productivity, and fatty acid composition of tropical microalgae for scale‐up production , 2010, Biotechnology and bioengineering.

[3]  René H Wijffels,et al.  Carotenoid and fatty acid metabolism in light‐stressed Dunaliella salina , 2010, Biotechnology and bioengineering.

[4]  S. Boussiba,et al.  The effect of light, salinity, and nitrogen availability on lipid production by Nannochloropsis sp. , 2011, Applied Microbiology and Biotechnology.

[5]  A. Ben‐Amotz,et al.  CHEMICAL PROFILE OF SELECTED SPECIES OF MICROALGAE WITH EMPHASIS ON LIPIDS 1 , 1985 .

[6]  Ana Cristina Oliveira,et al.  Microalgae as a raw material for biofuels production , 2009, Journal of Industrial Microbiology & Biotechnology.

[7]  R. Wijffels,et al.  Growth of oil accumulating microalga Neochloris oleoabundans under alkaline-saline conditions. , 2012, Bioresource technology.

[8]  Q. Hu,et al.  Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. , 2008, The Plant journal : for cell and molecular biology.

[9]  E. Cahoon,et al.  Metabolic and gene expression changes triggered by nitrogen deprivation in the photoautotrophically grown microalgae Chlamydomonas reinhardtii and Coccomyxa sp. C-169. , 2012, Phytochemistry.

[10]  J. Pruvost,et al.  Investigation of biomass and lipids production with Neochloris oleoabundans in photobioreactor. , 2009, Bioresource technology.

[11]  Li-Hua Cheng,et al.  Enhanced lipid production of Chlorella vulgaris by adjustment of cultivation conditions. , 2010, Bioresource technology.

[12]  Yang Kuang,et al.  Growth and neutral lipid synthesis in green microalgae: a mathematical model. , 2011, Bioresource technology.

[13]  K. Yamaberi,et al.  Limited feeding of potassium nitrate for intracellular lipid and triglyceride accumulation of Nannochloris sp. UTEX LB1999 , 2000, Applied Microbiology and Biotechnology.

[14]  Robert P. van Hille,et al.  Lipid productivity, settling potential and fatty acid profile of 11 microalgal species grown under nitrogen replete and limited conditions , 2011, Journal of Applied Phycology.

[15]  Karseno,et al.  Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells. , 2006, Journal of bioscience and bioengineering.

[16]  Mutsumi Takagi,et al.  Nitrogen depletion for intracellular triglyceride accumulation to enhance liquefaction yield of marine microalgal cells into a fuel oil , 1998 .

[17]  S. O. Lourenço,et al.  Effects of nitrogen starvation on the photosynthetic physiology of a tropical marine microalga Rhodomonas sp. (Cryptophyceae) , 2009 .

[18]  K. Cooksey,et al.  TRIGLYCERIDE ACCUMULATION AND FATTY ACID PROFILE CHANGES IN CHLORELLA (CHLOROPHYTA) DURING HIGH pH‐INDUCED CELL CYCLE INHIBITION 1 , 1990 .

[19]  Y. Chisti.  Biodiesel from microalgae. , 2007, Biotechnology advances.

[20]  Milton Sommerfeld,et al.  Photosynthetic carbon partitioning and lipid production in the oleaginous microalga Pseudochlorococcum sp. (Chlorophyceae) under nitrogen-limited conditions. , 2011, Bioresource technology.

[21]  S. Arad,et al.  Pigment and Structural Changes in Chlorella zofingiensis upon Light and Nitrogen Stress , 1995 .

[22]  C. Lan,et al.  Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans , 2008, Applied Microbiology and Biotechnology.

[23]  S. Harrison,et al.  Lipid productivity as a key characteristic for choosing algal species for biodiesel production , 2009, Journal of Applied Phycology.

[24]  J. Pruvost,et al.  Systematic investigation of biomass and lipid productivity by microalgae in photobioreactors for biodiesel application. , 2011, Bioresource technology.

[25]  C. Posten,et al.  Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production , 2008, BioEnergy Research.

[26]  L. Rodolfi,et al.  Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low‐cost photobioreactor , 2009, Biotechnology and bioengineering.

[27]  C. Wilhelm,et al.  A complete energy balance from photons to new biomass reveals a light- and nutrient-dependent variability in the metabolic costs of carbon assimilation. , 2007, Journal of experimental botany.

[28]  Maria J Barbosa,et al.  Microalgal production--a close look at the economics. , 2011, Biotechnology advances.

[29]  Z. Cohen,et al.  Microbial and algal oils: Do they have a future for biodiesel or as commodity oils? , 2008 .

[30]  K. Apt,et al.  COMMERCIAL DEVELOPMENTS IN MICROALGAL BIOTECHNOLOGY , 1999 .

[31]  Teresa M. Mata,et al.  Microalgae for biodiesel production and other applications: A review , 2010 .

[32]  M. Seibert,et al.  Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga , 2011, PloS one.