On the development of colloidal nanoparticles towards multifunctional structures and their possible use for biological applications.

In this Review, we describe the synthesis of high-quality colloidal nanoparticles in organic solvents, the mechanisms by which they can be transferred into aqueous solution, and some of their applications in biology. In particular, we will place emphasis on the creation of multifunctional nanoparticles or nanoparticle assemblies.

[1]  Andreas Kornowski,et al.  One-pot synthesis of highly luminescent CdSe/CdS core-shell nanocrystals via organometallic and greener chemical approaches , 2003 .

[2]  A. Rogach,et al.  Colloidal synthesis and self-assembly of CoPt(3) nanocrystals. , 2002, Journal of the American Chemical Society.

[3]  J. Storhoff,et al.  A DNA-based method for rationally assembling nanoparticles into macroscopic materials , 1996, Nature.

[4]  J. Jacobson,et al.  Synthesis of monofunctionalized gold nanoparticles by fmoc solid-phase reactions. , 2004, Journal of the American Chemical Society.

[5]  Andreas Kornowski,et al.  Highly Luminescent Monodisperse CdSe and CdSe/ZnS Nanocrystals Synthesized in a Hexadecylamine-Trioctylphosphine Oxide-Trioctylphospine Mixture. , 2001, Nano letters.

[6]  G. Galli,et al.  Solution Synthesis of Germanium Nanocrystals: Success and Open Challenges , 2004 .

[7]  U. Banin,et al.  Synthesis and Properties of CdSe/ZnS Core/Shell Nanorods , 2003 .

[8]  H. Mattoussi,et al.  Preparation of quantum dot-biotin conjugates and their use in immunochromatography assays. , 2003, Analytical chemistry.

[9]  J. Bacri,et al.  Intracellular uptake of anionic superparamagnetic nanoparticles as a function of their surface coating. , 2003, Biomaterials.

[10]  Christopher Andersen,et al.  The Name Game , 1977 .

[11]  Stephen G. Hickey,et al.  Highly Luminescent Water-Soluble CdTe Quantum Dots , 2003 .

[12]  Shimon Weiss,et al.  Bioactivation and cell targeting of semiconductor CdSe/ZnS nanocrystals with phytochelatin-related peptides. , 2004, Journal of the American Chemical Society.

[13]  Philippe Rostaing,et al.  Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking , 2003, Science.

[14]  M. Bruchez,et al.  Corrigendum: Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots , 2003, Nature Biotechnology.

[15]  A. Alivisatos,et al.  Synthesis of Colloidal Cobalt Nanoparticles with Controlled Size and Shapes , 2001 .

[16]  P. Schultz,et al.  Organization of 'nanocrystal molecules' using DNA , 1996, Nature.

[17]  Christine M. Micheel,et al.  Cell Motility and Metastatic Potential Studies Based on Quantum Dot Imaging of Phagokinetic Tracks , 2002 .

[18]  Kenji Yamamoto,et al.  Semiconductor quantum dot/albumin complex is a long-life and highly photostable endosome marker. , 2003, Biochemical and biophysical research communications.

[19]  George P. Anderson,et al.  Bioconjugation of Highly Luminescent Colloidal CdSe–ZnS Quantum Dots with an Engineered Two-Domain Recombinant Protein , 2001 .

[20]  J. Bonner,et al.  Differentiation , 1968, Nature.

[21]  A Paul Alivisatos,et al.  Room-temperature single-nucleotide polymorphism and multiallele DNA detection using fluorescent nanocrystals and microarrays. , 2003, Analytical chemistry.

[22]  J. Matthew Mauro,et al.  Long-term multiple color imaging of live cells using quantum dot bioconjugates , 2003, Nature Biotechnology.

[23]  M. Dahan,et al.  Time-gated biological imaging by use of colloidal quantum dots. , 2001, Optics letters.

[24]  Igor L. Medintz,et al.  Multiplexed toxin analysis using four colors of quantum dot fluororeagents. , 2004, Analytical chemistry.

[25]  D. Schiffrin,et al.  Self-Organization of Nanosized Gold Particles , 1998 .

[26]  Horst Weller,et al.  Ligand design and bioconjugation of colloidal gold nanoparticles. , 2002, Angewandte Chemie.

[27]  N. Seeman,et al.  Selfassembly of Metallic Nanoparticle Arrays by DNA Scaffolding , 2002 .

[28]  Weidong Yang,et al.  Linearly Polarized Emission from Colloidal Semiconductor Quantum Rods , 2001, Science.

[29]  D. Balding,et al.  HLA Sequence Polymorphism and the Origin of Humans , 2006 .

[30]  G. Albrecht-Buehler,et al.  Phagokinetic tracks of 3T3 cells: Parallels between the orientation of track segments and of cellular structures which contain actin or tubulin , 1977, Cell.

[31]  M. Farle,et al.  Synthesis and structure of colloidal bimetallic nanocrystals: The non-alloying system Ag/Co , 2002 .

[32]  Oliver Benson,et al.  Highly Emissive Colloidal CdSe/CdS Heterostructures of Mixed Dimensionality , 2003 .

[33]  Xiaogang Peng,et al.  Epitaxial Growth of Highly Luminescent CdSe/CdS Core/Shell Nanocrystals with Photostability and Electronic Accessibility , 1997 .

[34]  M. Bruchez,et al.  Optical coding of mammalian cells using semiconductor quantum dots. , 2004, Analytical biochemistry.

[35]  Thomas M. Jovin,et al.  Quantum dots finally come of age , 2003, Nature Biotechnology.

[36]  J. Cheon,et al.  Size and shape controlled ZnTe nanocrystals with quantum confinement effect , 2001 .

[37]  R. Murray,et al.  Monolayer-protected cluster molecules. , 2000, Accounts of chemical research.

[38]  M. Bawendi,et al.  (CdSe)ZnS Core-Shell Quantum Dots - Synthesis and Characterization of a Size Series of Highly Luminescent Nanocrystallites , 1997 .

[39]  M. Rosen,et al.  The Electronic Structure of Semiconductor Nanocrystals1 , 2000 .

[40]  Shimon Weiss,et al.  Synthesis and Properties of Biocompatible Water-Soluble Silica-Coated CdSe/ZnS Semiconductor Quantum Dots† , 2001 .

[41]  Chad A. Mirkin,et al.  One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle Probes , 1998 .

[42]  C. Murphy,et al.  Temperature- and Salt-Dependent Binding of Long DNA to Protein-Sized Quantum Dots: Thermodynamics of “Inorganic Protein”−DNA Interactions , 2000 .

[43]  G. Schmid,et al.  Die Komplexierung von Goldkolloiden , 1989 .

[44]  Xiaogang Peng,et al.  Formation of high-quality CdS and other II-VI semiconductor nanocrystals in noncoordinating solvents: tunable reactivity of monomers. , 2002, Angewandte Chemie.

[45]  J. Storhoff,et al.  Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. , 1997, Science.

[46]  Christine M. Micheel,et al.  Conformation of oligonucleotides attached to gold nanocrystals probed by gel-electrophoresis , 2003 .

[47]  E. Braun,et al.  DNA-templated assembly and electrode attachment of a conducting silver wire , 1998, Nature.

[48]  Erkki Ruoslahti,et al.  Nanocrystal targeting in vivo , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[49]  Zeev Rosenzweig,et al.  Superparamagnetic Fe2O3 Beads−CdSe/ZnS Quantum Dots Core−Shell Nanocomposite Particles for Cell Separation , 2004 .

[50]  C. Mirkin,et al.  Scanometric DNA array detection with nanoparticle probes. , 2000, Science.

[51]  Yi Lu,et al.  A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles. , 2003, Journal of the American Chemical Society.

[52]  J. Matthew Mauro,et al.  Self-Assembly of CdSe−ZnS Quantum Dot Bioconjugates Using an Engineered Recombinant Protein , 2000 .

[53]  M. Bruchez,et al.  Lighting up cells with quantum dots. , 2003, BioTechniques.

[54]  Gleb B. Sukhorukov,et al.  Stepwise Polyelectrolyte Assembly on Particle Surfaces: a Novel Approach to Colloid Design , 1998 .

[55]  B. Åkerman,et al.  Nonspecific and Thiol-Specific Binding of DNA to Gold Nanoparticles , 2003 .

[56]  Itamar Willner,et al.  Au-nanoparticle nanowires based on DNA and polylysine templates. , 2002, Angewandte Chemie.

[57]  A Paul Alivisatos,et al.  Sorting fluorescent nanocrystals with DNA. , 2002, Journal of the American Chemical Society.

[58]  Horst Weller,et al.  Biofunctionalization of Silica-Coated CdTe and Gold Nanocrystals , 2002 .

[59]  M. Bawendi,et al.  Synthesis and characterization of nearly monodisperse CdE (E = sulfur, selenium, tellurium) semiconductor nanocrystallites , 1993 .

[60]  A Paul Alivisatos,et al.  A single-electron transistor made from a cadmium selenide nanocrystal , 1997, Nature.

[61]  M. Ancona,et al.  Isolation and Characterization of Trioxyethylene-Encapsulated Gold Nanoclusters Functionalized with a Single DNA Strand , 2004 .

[62]  Xiaogang Peng,et al.  Formation of High Quality InP and InAs Nanocrystals in a Noncoordinating Solvent , 2002 .

[63]  Liberato Manna,et al.  Synthesis of Soluble and Processable Rod-, Arrow-, Teardrop-, and Tetrapod-Shaped CdSe Nanocrystals , 2000 .

[64]  Xiaogang Peng,et al.  Formation of high-quality CdTe, CdSe, and CdS nanocrystals using CdO as precursor. , 2001, Journal of the American Chemical Society.

[65]  Liberato Manna,et al.  Shape Control of Colloidal Semiconductor Nanocrystals , 2002 .

[66]  James McBride,et al.  Targeting cell surface receptors with ligand-conjugated nanocrystals. , 2002, Journal of the American Chemical Society.

[67]  A. Sutherland,et al.  Quantum dots as luminescent probes in biological systems , 2002 .

[68]  J. Davies,et al.  Molecular Biology of the Cell , 1983, Bristol Medico-Chirurgical Journal.

[69]  O. Urakawa,et al.  Small - , 2007 .

[70]  A Paul Alivisatos,et al.  DNA-Based Assembly of Gold Nanocrystals. , 1999, Angewandte Chemie.

[71]  H. Weller,et al.  Ligandendesign und Biokonjugation kolloidaler Gold‐Nanopartikel , 2002 .

[72]  Itamar Willner,et al.  Photoelectrochemistry with Controlled DNA-Cross-Linked CdS Nanoparticle Arrays This research is supported by The U.S.-Israel Binational Science Foundation. The Max Planck Research Award for International Cooperation (I.W.) is gratefully acknowledged. , 2001, Angewandte Chemie.

[73]  Weidong Yang,et al.  Shape control of CdSe nanocrystals , 2000, Nature.

[74]  M. Sastry,et al.  Phase transfer of silver nanoparticles from aqueous to organic solutions using fatty amine molecules. , 2003, Journal of colloid and interface science.

[75]  N. Seeman,et al.  Design and self-assembly of two-dimensional DNA crystals , 1998, Nature.

[76]  Sun,et al.  Monodisperse FePt nanoparticles and ferromagnetic FePt nanocrystal superlattices , 2000, Science.

[77]  J. Post,et al.  Quantum dot ligands provide new insights into erbB/HER receptor–mediated signal transduction , 2004, Nature Biotechnology.

[78]  S. Nie,et al.  Luminescent quantum dots for multiplexed biological detection and imaging. , 2002, Current opinion in biotechnology.

[79]  Christine M. Micheel,et al.  Electrophoretic Isolation of Discrete Au Nanocrystal/DNA Conjugates , 2001 .

[80]  C. Alexiou,et al.  Locoregional cancer treatment with magnetic drug targeting. , 2000, Cancer research.

[81]  A Paul Alivisatos,et al.  Quantum-dot-based cell motility assay. , 2003, Science's STKE : signal transduction knowledge environment.

[82]  E. Braun,et al.  DNA-Templated Carbon Nanotube Field-Effect Transistor , 2003, Science.

[83]  Chad A. Mirkin,et al.  Programmed Materials Synthesis with DNA. , 1999, Chemical reviews.

[84]  M. Casanove,et al.  Synthesis and Magnetic Properties of Nickel Nanorods , 2001 .

[85]  Mathias Brust,et al.  Synthesis of thiol-derivatised gold nanoparticles in a two-phase liquid-liquid system , 1994 .

[86]  Ma Hui,et al.  Quantum dot-labeled trichosanthin , 2000 .

[87]  Colin J. Loweth,et al.  DNA ALS GERUST ZUR BILDUNG VON AGGREGATEN AUS GOLD-NANOKRISTALLEN , 1999 .

[88]  P. Alivisatos The use of nanocrystals in biological detection , 2004, Nature Biotechnology.

[89]  F. Caruso,et al.  Phase transfer of surface-modified gold nanoparticles by hydrophobization with alkylamines , 2003 .

[90]  Uri Banin,et al.  Synthese und Charakterisierung von InAs/InP‐ und InAs/CdSe‐Kern/Schalen‐Nanokristallen , 1999 .

[91]  L. Liz‐Marzán,et al.  Synthesis and Optical Properties of Gold-Labeled Silica Particles , 1995 .

[92]  S. Bhatia,et al.  Probing the Cytotoxicity Of Semiconductor Quantum Dots. , 2004, Nano letters.

[93]  Zhong Lin Wang,et al.  Bimagnetic Core/Shell FePt/Fe3O4 Nanoparticles , 2004 .

[94]  Xiaogang Peng,et al.  Photochemical instability of CdSe nanocrystals coated by hydrophilic thiols. , 2001, Journal of the American Chemical Society.

[95]  Yoshio Kobayashi,et al.  Preparation and Properties of Silica-Coated Cobalt Nanoparticles† , 2003 .

[96]  Robert L. Letsinger,et al.  The DNA-Mediated Formation of Supramolecular Mono- and Multilayered Nanoparticle Structures , 2000 .

[97]  Chad A. Mirkin,et al.  Programmed Assembly of DNA Functionalized Quantum Dots , 1999 .

[98]  Liberato Manna,et al.  Controlled growth of tetrapod-branched inorganic nanocrystals , 2003, Nature materials.

[99]  M. Petruska,et al.  An amphiphilic approach to nanocrystal quantum dot-titania nanocomposites. , 2004, Journal of the American Chemical Society.

[100]  A. P. Alivisatos,et al.  Epitaxial growth and photochemical annealing of graded CdS/ZnS shells on colloidal CdSe nanorods. , 2002, Journal of the American Chemical Society.

[101]  Gleb B. Sukhorukov,et al.  NEUARTIGE POLYMERHOHLKORPER DURCH SELBSTORGANISATION VON POLYELEKTROLYTEN AUF KOLLOIDALEN TEMPLATEN , 1998 .

[102]  C. Tribet,et al.  Amphipols: polymers that keep membrane proteins soluble in aqueous solutions. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[103]  Joachim O. Rädler,et al.  Hydrophobic Nanocrystals Coated with an Amphiphilic Polymer Shell: A General Route to Water Soluble Nanocrystals , 2004 .

[104]  Xiaogang Peng,et al.  Kinetics of II-VI and III-V Colloidal Semiconductor Nanocrystal Growth: “Focusing” of Size Distributions , 1998 .

[105]  Wolfgang Pompe,et al.  DNA as a Selective Metallization Template , 2002 .

[106]  Uri Banin,et al.  Selective Growth of Metal Tips onto Semiconductor Quantum Rods and Tetrapods , 2004, Science.

[107]  Moungi G Bawendi,et al.  Oligomeric ligands for luminescent and stable nanocrystal quantum dots. , 2003, Journal of the American Chemical Society.

[108]  Bing Xu,et al.  Facile one-pot synthesis of bifunctional heterodimers of nanoparticles: a conjugate of quantum dot and magnetic nanoparticles. , 2004, Journal of the American Chemical Society.

[109]  G. Albrecht-Buehler,et al.  The phagokinetic tracks of 3T3 cells , 1977, Cell.

[110]  E. Shevchenko,et al.  Study of nucleation and growth in the organometallic synthesis of magnetic alloy nanocrystals: the role of nucleation rate in size control of CoPt3 nanocrystals. , 2003, Journal of the American Chemical Society.

[111]  A. Alivisatos,et al.  Synthesis, self-assembly, and magnetic behavior of a two-dimensional superlattice of single-crystal ε-Co nanoparticles , 2001 .

[112]  U. Ebels,et al.  Synthesis of Core−Shell PtCo Nanocrystals† , 2003 .

[113]  G. Albrecht-Buehler,et al.  Daughter 3T3 cells. Are they mirror images of each other? , 1977, The Journal of cell biology.

[114]  C. Bárcena,et al.  APPLICATIONS OF MAGNETIC NANOPARTICLES IN BIOMEDICINE , 2003 .

[115]  Zeev Rosenzweig,et al.  Synthesis of Glyconanospheres Containing Luminescent CdSe−ZnS Quantum Dots , 2003 .

[116]  Xiaogang Peng,et al.  Formation and stability of size-, shape-, and structure-controlled CdTe nanocrystals: Ligand effects on monomers and nanocrystals , 2003 .

[117]  M. Bruchez,et al.  Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots , 2003, Nature Biotechnology.

[118]  Vincent Noireaux,et al.  In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles , 2002, Science.

[119]  G. Schmid,et al.  The Complexation of Gold Colloids , 1989 .

[120]  C. Bustamante,et al.  Conjugation of DNA to Silanized Colloidal Semiconductor Nanocrystalline Quantum Dots , 2002 .

[121]  Hao Zeng,et al.  Monodisperse MFe2O4 (M = Fe, Co, Mn) nanoparticles. , 2004, Journal of the American Chemical Society.

[122]  L. Liz‐Marzán,et al.  Stabilization of CdS semiconductor nanoparticles against photodegradation by a silica coating procedure , 1998 .

[123]  S. Nie,et al.  Molecular profiling of single cells and tissue specimens with quantum dots. , 2003, Trends in biotechnology.

[124]  S. Pathak,et al.  Hydroxylated quantum dots as luminescent probes for in situ hybridization. , 2001, Journal of the American Chemical Society.

[125]  C. Mirkin,et al.  A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles. , 2000, Analytical chemistry.

[126]  M. Bawendi,et al.  Type-II quantum dots: CdTe/CdSe(core/shell) and CdSe/ZnTe(core/shell) heterostructures. , 2003, Journal of the American Chemical Society.

[127]  C. Niemeyer,et al.  Self-assembled nanostructures based on DNA: towards the development of nanobiotechnology. , 2000, Current opinion in chemical biology.

[128]  Cao,et al.  Synthesis and Characterization of InAs/InP and InAs/CdSe Core/Shell Nanocrystals. , 1999, Angewandte Chemie.

[129]  G. Sukhorukov,et al.  Luminescent polymer microcapsules addressable by a magnetic field. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[130]  L. Liz‐Marzán,et al.  Silica-coated metals and semiconductors. Stabilization and nanostructuring , 2000 .

[131]  C. Niemeyer,et al.  Site-selective immobilization of gold nanoparticles functionalized with DNA oligomers , 2001 .

[132]  S. Nie,et al.  Quantum dot bioconjugates for ultrasensitive nonisotopic detection. , 1998, Science.