molecular typing of Legionella pneumophila strains isolated from environment in Morocco.

Legionella pneumophila is a common cause of hospital and community-acquired pneumonia, being transmitted by inhalation of aqueous aerosols. Most legionellosis outbreaks are linked to contaminated hot water systems or cooling towers. The aim of this study was to determine the genetic diversity of (n= 55) environmental strains of L. pneumophila recovered from the hot water distribution systems of 16 establishments in seven Moroccan towns during the period 2009-2011. Thirteen chromosomal restriction patterns determined by Pulsed field gel electrophoresis were detected. The strains of L. pneumophila serogroup1 exhibited in 6/13 different PFGE patterns, while the strains of L. pneumophila serogroups 2-14 showed 7/13 PFGE patterns. The PFGE showed the existence of various patterns in Morocco, The pattern -XI- have tree similar profiles with the endemic L. pneumophila Paris's strain. This technique also allowed to conclude that the same pulsotype was found for many strains isolated from different establishments. Moreover, different pulsolypes were found for strains isolated from the same establishment. These results showed that PFGE analysis is a powerful tool to reveal the clonal nature and genetic differences among L. pneumophila strains.

[1]  L. Slim-Saidi,et al.  Comparaison moléculaire de Legionella pneumophila sérogroupe 1 isolées en Tunisie , 2008 .

[2]  C. Campèse,et al.  Clusters of travel-associated Legionnaires disease in Italy, Spain and France, July 2002 - June 2006. , 2007, Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin.

[3]  T. Ezaki,et al.  Legionella impletisoli sp. nov. and Legionella yabuuchiae sp. nov., isolated from soils contaminated with industrial wastes in Japan. , 2007, Systematic and applied microbiology.

[4]  M. Triassi,et al.  Clinical and environmental distribution of Legionella pneumophila in a university hospital in Italy: efficacy of ultraviolet disinfection. , 2006, The Journal of hospital infection.

[5]  M. Maurin,et al.  Antibiotic susceptibilities of Legionella pneumophila strain Paris in THP-1 cells as determined by real-time PCR assay. , 2005, The Journal of antimicrobial chemotherapy.

[6]  J. Etienne,et al.  Consensus Sequence-Based Scheme for Epidemiological Typing of Clinical and Environmental Isolates of Legionella pneumophila , 2005, Journal of Clinical Microbiology.

[7]  M. García-nuñez,et al.  Environmental Cultures and Hospital-Acquired Legionnaires' Disease: A 5-Year Prospective Study in 20 Hospitals in Catalonia, Spain , 2004, Infection Control & Hospital Epidemiology.

[8]  C. Campèse,et al.  Clusters of travel associated legionnaires' disease in France, September 2001- August 2003. , 2004, Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin.

[9]  F. Vandenesch,et al.  Clinical and Environmental Distributions of Legionella Strains in France Are Different , 2004, Journal of Clinical Microbiology.

[10]  K. Yamaguchi,et al.  A large outbreak of Legionnaires' disease due to an inadequate circulating and filtration system for bath water--epidemiologic manifestations. , 2003, Internal medicine.

[11]  C. Campèse,et al.  Legionella pneumophila Serogroup 1 Strain Paris: Endemic Distribution throughout France , 2003, Journal of Clinical Microbiology.

[12]  P. Visca,et al.  Designation of the European Working Group on Legionella Infection (EWGLI) Amplified Fragment Length Polymorphism Types of Legionella pneumophila Serogroup 1 and Results of Intercentre Proficiency Testing Using a Standard Protocol , 2002, European Journal of Clinical Microbiology and Infectious Diseases.

[13]  T. File,et al.  Distribution of Legionella species and serogroups isolated by culture in patients with sporadic community-acquired legionellosis: an international collaborative survey. , 2002, The Journal of infectious diseases.

[14]  Barry S. Fields,et al.  Legionella and Legionnaires' Disease: 25 Years of Investigation , 2002, Clinical Microbiology Reviews.

[15]  Michael Steinert,et al.  Legionella pneumophila: an aquatic microbe goes astray. , 2002, FEMS microbiology reviews.

[16]  U. Buchholz,et al.  Strengths and limitations of molecular subtyping in a community outbreak of Legionnaires' disease , 2000, Epidemiology and Infection.

[17]  C. Goulvestre,et al.  Single Clonal Origin of a High Proportion of Legionella pneumophila Serogroup 1 Isolates from Patients and the Environment in the Area of Paris, France, over a 10-Year Period , 1999, Journal of Clinical Microbiology.

[18]  J. Etienne,et al.  A multicenter evaluation of genotypic methods for the epidemiologic typing of Legionella pneumophila serogroup 1: results of a pan-European study. , 1999, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[19]  R. Leclercq,et al.  Molecular typing of Legionella pneumophila serogroup 1 isolates from patients and the nosocomial environment by arbitrarily primed PCR and pulsed-field gel electrophoresis. , 1999, Journal of medical microbiology.

[20]  C. Whitney,et al.  Comparison of Legionella pneumophila isolates by arbitrarily primed PCR and pulsed-field gel electrophoresis: analysis from seven epidemic investigations , 1995, Journal of clinical microbiology.

[21]  M. Assmann,et al.  Epidemiologic investigation by macrorestriction analysis and by using monoclonal antibodies of nosocomial pneumonia caused by Legionella pneumophila serogroup 10 , 1994, Journal of clinical microbiology.

[22]  N. Cohen,et al.  Enquête Epidémiologique sur la Légionellose et Prévalence de Legionella pneumophila dans Les Eaux Chaudes Sanitaires au Maroc , 2009 .

[23]  J. Freney,et al.  [Molecular comparison of Legionella pneumophila serogroup 1 isolated in Tunisia]. , 2008, Pathologie-biologie.

[24]  K. Ko,et al.  Legionella busanensis sp. nov., isolated from cooling tower water in Korea. , 2003, International journal of systematic and evolutionary microbiology.

[25]  M. Tumbarello,et al.  Diffusione ambientale di Legionella spp e frequenza di legionellosi in pazienti affetti da polmonite: primi risultati di uno studio multicentrico italiano , 2003 .