Amperometric lactate biosensor based on carbon paste electrode modified with benzo[c]cinnoline and multiwalled carbon nanotubes

Amperometric lactate biosensor based on a carbon paste electrode modified with benzo[c]cinnoline and multiwalled carbon nanotubes is reported. Incorporation of benzo[c]cinnoline acting as a mediator and multiwalled carbon nanotubes providing a conduction pathway to accelerate electron transfer due to their excellent conductivity into carbon paste matrix resulted in a high performance lactate biosensor. The resulting biosensor exhibited a fast response, high selectivity, good repeatability and storage stability. Under the optimal conditions, the enzyme electrode showed the detection limit of 7.0×10−8 M with a linear range of 2.0×10−7 M–1.1×10−4 M. The usefulness of the biosensor was demonstrated in serum samples.

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