TNF-Anti-schistosomular activity of human monocytes / macrophages in response to interleukin-3 and granulocyte-macrophage colony-stimulating factor stimulation

HUMAN monocytes, co-incubated for 7 days in culture with GM-CSF or IL-3 but not with IFN-T, exerted a variable schistosotnulicidal effect on Schistosoma mansoni parasites when grown in 96-well round-bottomed plates but not in flat-bottomed plates. Addition of LPS or IFN-’{ or both, for the last 48 h did not enhance the cidal effect. Addition of LPS but not IFN-T to the pre-incubated cells with GM-CSF or IL-3 markedly stimulated TNFproduction by the cells but not their cidal activity. The variable cidal effects obtained with the monocytes/macrophages from different donors suggest that these effects may be genetically predetermined and are possibly linked to blood group markers or to MHC class I or II antigens.

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