Sucrase-Isomaltase Gene Expression and Adenocarcinoma

Background: Specialized Barrett’s esophageal mucosa, characterized by incomplete intestinal metaplasia of the esophageal mucosa, is associated with the development of adenocarcinoma. Although the intestinal disaccharidase sucrase-isomaltase (SI) has been shown in incomplete intestinal metaplasia of the stomach, it is commonly believed that Barrett’s mucosa does not express SI based on the lack of enzymatic activity. This study was undertaken to determine whether the SI gene is expressed in Barrett’s epithelium and its associated adenocarcinoma at the leve1 of messenger RNA (mRNA) and protein. Methods: Reverse transcription polymerase chain reaction was used to determine the presence of SI mRNA in Barrett’s esophagus and esophageal adenocarcinomas. Cellular localization of SI protein was determined by immunohistochemistry. Results: SI mRNA was identified in 76% of Barrett’s epithelium and 82% of esophageal adenocarcinomas. The transcriptional initiation site for SI in these tissues was identical to that of the smal1 intestine. Immunohistochemical localization showed that SI was directed to the apical membrane in Barrett’s epithelium in contrast to a more diffuse cytoplasmic pattern in esophageal adenocarcinomas. Conclusions: Columnar cells of specialized Barrett’s epithelium express SI and are, therefore, phenotypically similar to those in incomplete intestinal metaplasia of the stomach with respect to intestinal gene expression.

[1]  P. Traber,et al.  Novel DNA-binding proteins regulate intestine-specific transcription of the sucrase-isomaltase gene , 1992, Molecular and cellular biology.

[2]  C. Andrews,et al.  Sucrase-isomaltase expression in chronic ulcerative colitis and dysplasia. , 1992, Human pathology.

[3]  B. Reid,et al.  Persistent clonal areas and clonal expansion in Barrett's esophagus. , 1992, Cancer research.

[4]  P. Traber,et al.  Isolation and characterization of the human sucrase-isomaltase gene and demonstration of intestine-specific transcriptional elements. , 1992, The Journal of biological chemistry.

[5]  B. Reid,et al.  Barrett's esophagus and esophageal adenocarcinoma. , 1991, Gastroenterology clinics of North America.

[6]  J. Roth,et al.  p53 gene mutations in Barrett's epithelium and esophageal cancer. , 1991, Cancer research.

[7]  R. Ashmun,et al.  Separate promoters control transcription of the human aminopeptidase N gene in myeloid and intestinal epithelial cells. , 1991, The Journal of biological chemistry.

[8]  J. Fraumeni,et al.  Rising incidence of adenocarcinoma of the esophagus and gastric cardia. , 1991, JAMA.

[9]  M. Indelli,et al.  Barrett esophagus after chemotherapy with cyclophosphamide, methotrexate, and 5-fluorouracil (CMF): an iatrogenic injury? , 1991, Annals of internal medicine.

[10]  M. Loeffler,et al.  Stem cells: attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt. , 1990, Development.

[11]  B. Reid,et al.  Specialized metaplastic columnar epithelium in Barrett's esophagus. A comparative transmission electron microscopic study. , 1989, Laboratory investigation; a journal of technical methods and pathology.

[12]  B. Ponder,et al.  Development of the pattern of cell renewal in the crypt-villus unit of chimaeric mouse small intestine. , 1988, Development.

[13]  B. A. J. Ponder,et al.  A clonal marker induced by mutation in mouse intestinal epithelium , 1988, Nature.

[14]  B. Dahms,et al.  Barrett's esophagus in three children after antileukemia chemotherapy , 1987, Cancer.

[15]  C. Boland,et al.  Glycoconjugate expression in normal, metaplastic, and neoplastic human upper gastrointestinal mucosa. , 1987, The Journal of clinical investigation.

[16]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[17]  R. R. Smith,et al.  The relationship between columnar epithelial dysplasia and invasive adenocarcinoma arising in Barrett's esophagus. , 1987, American journal of clinical pathology.

[18]  A. Zweibaum,et al.  A and H blood group antigens as markers of sucrase-isomaltase from the enterocyte-like differentiated human colon carcinoma cell lines HT-29 and Caco-2. , 1987, Cancer research.

[19]  W. Olsen,et al.  A study of the molecular pathology of sucrase-isomaltase deficiency. A defect in the intracellular processing of the enzyme. , 1987, The New England journal of medicine.

[20]  David F. Cruess,et al.  Barrett's esophagus: A prevalent, occult complication of gastroesophageal reflux disease , 1987 .

[21]  S. Povey,et al.  Isolation of a cDNA probe for a human jejunal brush-border hydrolase, sucrase-isomaltase, and assignment of the gene locus to chromosome 3. , 1987, Gene.

[22]  H. Korsmo,et al.  Localization of sucrase-isomaltase in the rat enterocyte. , 1987, Gastroenterology.

[23]  U. Schibler,et al.  Alternative promoters in developmental gene expression. , 1987, Annual review of genetics.

[24]  W S Payne,et al.  The incidence of adenocarcinoma in columnar-lined (Barrett's) esophagus. , 1985, The New England journal of medicine.

[25]  B. Moats-Staats,et al.  Nucleotide sequence analysis of a cDNA encoding human ubiquitin reveals that ubiquitin is synthesized as a precursor. , 1985, The Journal of biological chemistry.

[26]  B. Ponder,et al.  Derivation of mouse intestinal crypts from single progenitor cells , 1985, Nature.

[27]  J. J. Thompson,et al.  Barrett's metaplasia and adenocarcinoma of the esophagus and gastroesophageal junction. , 1983, Human pathology.

[28]  T. Hirota,et al.  Distribution of marker enzymes and mucin in intestinal metaplasia in human stomach and relation to complete and incomplete types of intestinal metaplasia to minute gastric carcinomas. , 1980, Journal of the National Cancer Institute.

[29]  W. Meyer,et al.  Barrett-esophagus Following Total Gastrectomy*: A Contribution to it's Pathogenesis , 1979, Endoscopy.

[30]  H. Watanabe.  Experimentally induced intestinal metaplasia in Wistar rats by X-ray irradiation. , 1978, Gastroenterology.

[31]  M. Classen,et al.  Disaccharidases of the gastric mucosa in chronic atrophic gastritis with intestinal metaplasia. , 1968, Digestion.

[32]  Barrett Nr,et al.  The lower esophagus lined by columnar epithelium. , 1957 .

[33]  P. Allison,et al.  The Oesophagus Lined with Gastric Mucous Membrane , 1953, Thorax.