Generic model control of an Escherichia coli fed-batch culture

Generic model control (GMC), a model-based adaptive control strategy, is applied to a fed-batch high cell density cultivation of Escherichia coli, with biomass production maximization as an objective. A macroscopic model based on the overflow metabolism assumption is used, considering two metabolic pathways (respirative and respiro-fermentative), and consisting of a set of nonlinear differential equations. A linear Kalman filter is designed to reconstruct the assumed unmeasured variables required to calculate the control law. Since the bioprocess model may present uncertainties, the robustness of the approach is tested through a set of Monte-Carlo simulations.

[1]  Rimvydas Simutis,et al.  Generic model control of the specific growth rate in recombinant Escherichia coli cultivations. , 2006, Journal of biotechnology.

[2]  Peter L. Lee,et al.  Nonlinear Process Control:: Applications Of Generic Model Control , 2011 .

[3]  Laurent Dewasme,et al.  Parameter estimation of a dynamic model of Escherichia coli fed-batch cultures , 2018 .

[4]  Didier Dumur,et al.  Regulation of lactic acid concentration in its bioproduction from wheat flour , 2016 .

[5]  S. Lee,et al.  High cell-density culture of Escherichia coli. , 1996, Trends in biotechnology.

[6]  Peter L. Lee,et al.  Generic model control — A case study , 1989 .

[7]  Govind Rao,et al.  Generic model control of induced protein expression in high cell density cultivation of Escherichia coli using on-line GFP-fusion monitoring , 2001 .

[8]  Eugénio C. Ferreira,et al.  Model-based adaptive control of acetate concentration during the production of recombinant proteins , 2002 .

[9]  Didier Dumur,et al.  Nonlinear Model Predictive Control applied to E. Coli Cultures , 2008 .

[10]  Greg Welch,et al.  Welch & Bishop , An Introduction to the Kalman Filter 2 1 The Discrete Kalman Filter In 1960 , 1994 .

[11]  B Sonnleitner,et al.  Growth of Saccharomyces cerevisiae is controlled by its limited respiratory capacity: Formulation and verification of a hypothesis , 1986, Biotechnology and bioengineering.

[12]  Michel Perrier,et al.  Extremum-seeking algorithm design for fed-batch cultures of microorganisms with overflow metabolism , 2011 .

[13]  Anne Richelle,et al.  Linear robust control of S. cerevisiae fed-batch cultures at different scales , 2010 .

[14]  B Sonnleitner,et al.  Growth characteristics of Escherichia coli HB101[pGEc47] on defined medium. , 1998, Biotechnology and bioengineering.

[15]  G. R. Sullivan,et al.  Generic model control (GMC) , 1988 .

[16]  Didier Dumur,et al.  Hierarchical Control Strategy based on Robust MPC and Integral Sliding mode - Application to a Continuous Photobioreactor , 2015 .

[17]  J. Liao,et al.  Escherichia coli as a host for metabolic engineering. , 2018, Metabolic engineering.

[18]  W. Deckwer,et al.  High cell density cultivation of Escherichia coli at controlled specific growth rate. , 1991, Journal of biotechnology.

[19]  Didier Dumur,et al.  A practical robust control scheme for yeast fed-batch cultures – An experimental validation , 2006 .

[20]  Alain Vande Wouwer,et al.  Nonlinear model predictive control of fed-batch cultures of micro-organisms exhibiting overflow metabolism: Assessment and robustness , 2012, Comput. Chem. Eng..