Genetic Diversity Analysis in Four Species of Satureja Spp and Gontcharovia Popovii using Morphological Traits and Molecular RAPD Markers

In order to evaluate the genetic diversity in 4 species of Satureja Spp and one species of Gontcharovia Popovii, 90 genotypes of 5 species were exploited using morphological traits and molecular RAPD markers. These species included Satureja bachtiarica Bunge (15 genotypes), Satureja mutica (15 genotypes), Satureja sahendica (12 genotypes), Satureja hortensis (24 genotypes) and Gontcharovia Popovii (24 genotypes). DNA was isolated from young leaflet tissue of the plant. Next, DNA was quantified using spectrophotometer (Pharmacia) and its quality was determined by electrophoresis on 0.7 % agarose gel. Finally, qualified DNAs were diluted for 25 µL reaction. After completion of DNA amplification in the polymerase chain reaction, DNA loaded by electrophoresis on 1.2 % agarose gel in 95 V TAE 1X buffer for 70 minutes. Genotypes were grouped into two and three clusters based on morphological traits and polymorphic obtained by RAPD markers, respectively. Out of 40 RAPD primers, 11 appropriate polymorphic primers were observed. A total of 49 polymorphic DNA bands generated with a mean of 4.45 bands per primer was obtained. The most polymorphic and the least polymorphic bands were seen in P7 and P1 primers, respectively. In this study, the average number of polymorphic bands per primer was 0.156. The highest (0.240) and lowest(0.060) number of polymorphic bands was achieved with P2 and P9 primers, respectively. Average marker index any primer in this test 1.449 was calculated so that the primer P7 (2.589) the maximum amount of primer P9 (0.781) were allocated to the lowest. According to the obtained data, RAPD markers could be useful tools for investigation of genetic diversity among species of Satureja Spp.

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