Adverse effects of bisphenol A on reproductive physiology in male goldfish at environmentally relevant concentrations.

Alternations of reproductive physiology were studied in the male goldfish (Carassius auratus L.) exposed to environmentally relevant concentrations (0.6, 4.5 and 11.0 μg/L) of bisphenol A (BPA) at days 10, 20 and 30 after exposure. Significant effects of BPA concentration, exposure time and their interactions were observed on testosterone (T), 11-ketotestosterone (11-KT) and sperm motility and velocity, but gonadosomatic index (GSI), hepatosomatic index (HSI) and 17β-estradiol (E(2)) were not affected. Vitellogenin (VTG) was only affected by BPA concentration. The T and 11-KT levels were significantly decreased in the BPA-treated groups after 20 or 30 days. Sperm motility was significantly decreased at 15, 30, 60 and 90 s post-activation in the BPA-treated groups after 20 or 30 days. But, significant decrease in sperm velocity was observed at 30, 60 and 90 s post-activation in the BPA-treated groups at all exposure times. The VTG was significantly increased in the males exposed to 11.0 μg/L at day 30 after exposure. The GSI, HSI and E(2) did not differ between the BPA-treated groups and control. The present study shows that the decrease of sperm quality is concurrent with the decrease of androgens and increase of VTG. The results suggest adverse effects of BPA on sperm motility and velocity via modifications of testicular steroidogenesis that might correspond to alternation in sperm maturation.

[1]  S. Chattopadhyay,et al.  Antiandrogenic effects of bisphenol A and nonylphenol on the function of androgen receptor. , 2003, Toxicological sciences : an official journal of the Society of Toxicology.

[2]  H. Shiratsuchi,et al.  Toxicity to early life stages and an estrogenic effect of a bisphenol A metabolite, 4-methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene on the medaka (Oryzias latipes). , 2005, Life sciences.

[3]  J. Oehlmann,et al.  A critical evaluation of the environmental risk assessment for plasticizers in the freshwater environment in Europe, with special emphasis on bisphenol A and endocrine disruption. , 2008, Environmental research.

[4]  Pedersen,et al.  Estrogenic response of bisphenol A in rainbow trout (Oncorhynchus mykiss). , 2000, Aquatic Toxicology.

[5]  J. Corton,et al.  Interaction of Estrogenic Chemicals and Phytoestrogens with Estrogen Receptor β. , 1998, Endocrinology.

[6]  M. Hardy,et al.  Inhibition of testicular steroidogenesis by the xenoestrogen bisphenol A is associated with reduced pituitary luteinizing hormone secretion and decreased steroidogenic enzyme gene expression in rat Leydig cells. , 2004, Endocrinology.

[7]  V. Žlábek,et al.  Sex Differentiation and Vitellogenin and 11-Ketotestosterone Levels in Chub, Leuciscus cephalus L., Exposed to 17 β-Estradiol and Testosterone During Early Development , 2009, Bulletin of environmental contamination and toxicology.

[8]  P. Mathur,et al.  Induction of oxidative stress by bisphenol A in the epididymal sperm of rats. , 2003, Toxicology.

[9]  J. Sumpter,et al.  Reproductive effects of long-term exposure to Bisphenol A in the fathead minnow (Pimephales promelas). , 2001, Environmental science & technology.

[10]  T. Policar,et al.  In vitro effects of Bisphenol A on sperm motility characteristics in Perca fluviatilis L. (Percidae; Teleostei) , 2010 .

[11]  Kevin W. Gaido,et al.  Bisphenol A interacts with the estrogen receptor α in a distinct manner from estradiol , 1998, Molecular and Cellular Endocrinology.

[12]  J. Sumpter,et al.  Measurement of vitellogenin, a biomarker for exposure to oestrogenic chemicals, in a wide variety of cyprinid fish , 1996, Journal of Comparative Physiology B.

[13]  T. Miura,et al.  The role of hormones in the acquisition of sperm motility in salmonid fish. , 1992, The Journal of experimental zoology.

[14]  P. Shin,et al.  Toxicity of bisphenol A and its bioaccumulation and removal by a marine microalga Stephanodiscus hantzschii. , 2009, Ecotoxicology and environmental safety.

[15]  I. Kang,et al.  Effects of bisphenol a on the reproduction of Japanese medaka (Oryzias latipes) , 2002, Environmental toxicology and chemistry.

[16]  T. Toth,et al.  Semen quality and sperm DNA damage in relation to urinary bisphenol A among men from an infertility clinic. , 2010, Reproductive toxicology.

[17]  B. Larsen,et al.  Comparison of protein expression in plasma from nonylphenol and bisphenol A-exposed Atlantic cod (Gadus morhua) and turbot (Scophthalmus maximus) by use of SELDI-TOF. , 2006, Aquatic toxicology.

[18]  Y. Katayama,et al.  Bisphenol A in the Aquatic Environment and Its Endocrine-Disruptive Effects on Aquatic Organisms , 2007, Critical reviews in toxicology.

[19]  P. J. Babin,et al.  The fish oocyte : from basic studies to biotechnological applications , 2007 .

[20]  E. Nelson,et al.  Functional significance of nuclear estrogen receptor subtypes in the liver of goldfish. , 2010, Endocrinology.

[21]  L. R. Harris,et al.  A review of the environmental fate, effects, and exposures of bisphenol A. , 1998, Chemosphere.

[22]  M. Boubelík,et al.  Effect of an Endocrine Disruptor on Mammalian Fertility. Application of Monoclonal Antibodies against Sperm Proteins as Markers for Testing Sperm Damage , 2002, American journal of reproductive immunology.

[23]  H. Budzinski,et al.  Alteration of Steroid Hormone Balance in Juvenile Turbot (Psetta maxima) Exposed to Nonylphenol, Bisphenol A, Tetrabromodiphenyl Ether 47, Diallylphthalate, Oil, and Oil Spiked with Alkylphenols , 2006, Archives of environmental contamination and toxicology.

[24]  Nurhidayat,et al.  Gonadosomatic index and testis morphology of common carp (Cyprinus carpio) in rivers contaminated with estrogenic chemicals. , 2002, The Journal of veterinary medical science.

[25]  Taisen Iguchi,et al.  An ecological assessment of bisphenol-A: evidence from comparative biology. , 2007, Reproductive toxicology.

[26]  Ana M Soto,et al.  Bisphenol-A and the great divide: a review of controversies in the field of endocrine disruption. , 2009, Endocrine reviews.

[27]  L. Folmar,et al.  Vitellogenin induction and reduced serum testosterone concentrations in feral male carp (Cyprinus carpio) captured near a major metropolitan sewage treatment plant. , 1996, Environmental health perspectives.

[28]  P. Fontaine,et al.  Influence of pre-inductive photoperiod variations on Eurasian perch Perca fluviatilis broodstock response to an inductive photothermal program , 2006 .

[29]  F. Lahnsteiner,et al.  Effect of bisphenol A on maturation and quality of semen and eggs in the brown trout, Salmo trutta f. fario. , 2005, Aquatic toxicology.

[30]  J. Cosson,et al.  Frenetic activation of fish spermatozoa flagella entails short-term motility, portending their precocious decadence. , 2010, Journal of fish biology.

[31]  T. Policar,et al.  Mechanism of action of mercury on sperm morphology, adenosine triphosphate content, and motility in Perca fluviatilis (Percidae; Teleostei) , 2011, Environmental toxicology and chemistry.

[32]  P. Bjerregaard,et al.  Sex hormone concentrations and gonad histology in brown trout (Salmo trutta) exposed to 17β-estradiol and bisphenol A , 2008, Ecotoxicology.

[33]  Cheryl S Watson,et al.  In vitro molecular mechanisms of bisphenol A action. , 2007, Reproductive toxicology.

[34]  E. Benfenati,et al.  In vivo exposure of carp to graded concentrations of bisphenol A. , 2007, General and comparative endocrinology.

[35]  S. Goodbred,et al.  Morphometric and Histopathological Parameters of Gonadal Development in Adult Common Carp from Contaminated and Reference Sites in Lake Mead, Nevada , 2003 .

[36]  Nobuaki Tominaga,et al.  Short-term effects of endocrine-disrupting chemicals on the expression of estrogen-responsive genes in male medaka (Oryzias latipes). , 2005, Aquatic toxicology.

[37]  M. Sepúlveda,et al.  ECOTOXICOLOGICAL EFFECTS OF ENDOCRINE DISRUPTING COMPOUNDS ON FISH REPRODUCTION , 2007 .