Gut Microbes and Microbial Metabolites in Colorectal Cancer Complicat Ed With Different Serum Albumin Levels

Background: Patients with colorectal cancer (CRC) are at risk of malnutrition. Gut microbes and microbial metabolites are involved in the initiation and development of CRC.Aims: To investigate serum protein levels in CRC patients and explore the role of gut microbes and microbial metabolites in CRCs complicated with different serum albumin levels.Methods: Overall, 398 CRC patients and same number of healthy volunteers in Huzhou Central Hospital from January 2016 to December 2018 were recruited to compare serum protein levels. A total of 30 and 56 stool samples from CRC patients were used to detect intestinal microbes and microbial metabolites, respectively. Bacterial 16S V3-V4 and fungal ITS ribosomal DNA genes were sequenced and gas chromatography/mass spectrometry (GC/MS) was performed to detect microbial metabolites. Results: Some serum protein-related indicators in the CRC group were lower than those in the control group (p＜0.05). The total protein and albumin levels in colon cancer patients were lower than those in rectal cancer patients (p＜0.05). The higher abundance of Sutterella is correlated with lower serum albumin level in CRCs. There were statistically significant differences in the abundance of fungi including Agaricomycetes， Simplicillium, Sclerotiniaceae, and Exophiala among patients with different serum albumin levels. Multiple gut bacteria and fungi are closely related to serum albumin levels.We found some characteristic microbial metabolites in CRCs complicated with different serum protein levels.Conclusions: The different serum albumin levels were associated with the gut microbes and microbial metabolites in CRCs.Trial registration: Correlation analysis of intestinal flora and its metabolites with tumor nutritional status in patients with colorectal cancer,ChiCTR1900022460,Registered 12 April 2019,http://www.chictr.org.cn/edit.aspx?pid=37950&htm=4

[1]  Gang Wang,et al.  Role of SCFAs in gut microbiome and glycolysis for colorectal cancer therapy , 2019, Journal of cellular physiology.

[2]  D. Vodnar,et al.  Gut Prevotella as a possible biomarker of diet and its eubiotic versus dysbiotic roles: a comprehensive literature review , 2019, British Journal of Nutrition.

[3]  M. Magnani,et al.  Effects in Cancer Cells of the Recombinant l-Methionine Gamma-Lyase from Brevibacterium aurantiacum. Encapsulation in Human Erythrocytes for Sustained l-Methionine Elimination , 2019, The Journal of Pharmacology and Experimental Therapeutics.

[4]  K. Faber,et al.  Short Chain Fatty Acids (SCFAs)-Mediated Gut Epithelial and Immune Regulation and Its Relevance for Inflammatory Bowel Diseases , 2019, Front. Immunol..

[5]  Bangmao Wang,et al.  Interplay between bile acids and the gut microbiota promotes intestinal carcinogenesis , 2019, Molecular carcinogenesis.

[6]  A. Barzegari,et al.  Apoptotic Effect of Saccharomyces cerevisiae on Human Colon Cancer SW480 Cells by Regulation of Akt/NF-ĸB Signaling Pathway , 2019, Probiotics and Antimicrobial Proteins.

[7]  H. Chuang,et al.  The germ-free mice monocolonization with Bacteroides fragilis improves azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer , 2019, Immunopharmacology and immunotoxicology.

[8]  Xinxiang Li,et al.  Survival Benefit of Preoperative Versus Postoperative Radiotherapy in Metastatic Rectal Cancer Treated With Definitive Surgical Resection of Primary Tumor: A Population Based, Propensity Score-Matched Study , 2019, Journal of Cancer.

[9]  Shuwen Han,et al.  Intestinal microorganisms involved in colorectal cancer complicated with dyslipidosis , 2018, Cancer biology & therapy.

[10]  H. Shim,et al.  Differences Regarding the Molecular Features and Gut Microbiota Between Right and Left Colon Cancer , 2018, Annals of coloproctology.

[11]  B. Han,et al.  Adoptive Cell Transfer: Is it a Promising Immunotherapy for Colorectal Cancer? , 2018, Theranostics.

[12]  C. Tsao,et al.  Effectiveness of the Multidisciplinary Team Model in Treating Colorectal Cancer , 2018, Gastroenterology nursing : the official journal of the Society of Gastroenterology Nurses and Associates.

[13]  F. Ogita,et al.  Within-Day Amino Acid Intakes and Nitrogen Balance in Male Collegiate Swimmers during the General Preparation Phase , 2018, Nutrients.

[14]  M. Kleerebezem,et al.  Metatranscriptome analysis of the microbial fermentation of dietary milk proteins in the murine gut , 2018, PloS one.

[15]  M. Vettore,et al.  The contribution of muscle, kidney, and splanchnic tissues to leucine transamination in humans. , 2018, Canadian journal of physiology and pharmacology.

[16]  C. Stefanelli,et al.  Influence of dietary protein and fructooligosaccharides on fecal fermentative end-products, fecal bacterial populations and apparent total tract digestibility in dogs , 2018, BMC Veterinary Research.

[17]  Shuwen Han,et al.  Role of intestinal flora in colorectal cancer from the metabolite perspective: a systematic review , 2018, Cancer management and research.

[18]  U. Gunnarsson,et al.  Prediction of outcome after curative surgery for colorectal cancer: preoperative haemoglobin, C‐reactive protein and albumin , 2018, Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland.

[19]  E. Ejdys,et al.  Share of the Saccharomyces genus in the mycobiota of the gastrointestinal tract of oncology patients – potential effects of a fruit-based diet , 2018, Annals of parasitology.

[20]  J. García-Mena,et al.  Gut microbiome production of short-chain fatty acids and obesity in children , 2018, European Journal of Clinical Microbiology & Infectious Diseases.

[21]  M. Marzorati,et al.  The response of canine faecal microbiota to increased dietary protein is influenced by body condition , 2017, BMC Veterinary Research.

[22]  S. Ogino,et al.  The role of intestinal bacteria in the development and progression of gastrointestinal tract neoplasms. , 2017, Surgical oncology.

[23]  R. Amdur,et al.  Preoperative hypoalbuminemia is associated with worse outcomes in colon cancer patients. , 2017, Clinical nutrition.

[24]  C. Mu,et al.  Temporal microbiota changes of high-protein diet intake in a rat model. , 2017, Anaerobe.

[25]  Kepeng Wang,et al.  Microbiome, inflammation and colorectal cancer. , 2017, Seminars in immunology.

[26]  C. Mu,et al.  An increase in corn resistant starch decreases protein fermentation and modulates gut microbiota during in vitro cultivation of pig large intestinal inocula , 2017, Animal nutrition.

[27]  R. Krishnamurthy,et al.  Nitrogen isotopes provide clues to amino acid metabolism in human colorectal cancer cells , 2017, Scientific Reports.

[28]  Yi Luo,et al.  Remodeling of energy metabolism by a ketone body and medium-chain fatty acid suppressed the proliferation of CT26 mouse colon cancer cells , 2017, Oncology letters.

[29]  A. Jemal,et al.  Colorectal cancer statistics, 2017 , 2017, CA: a cancer journal for clinicians.

[30]  R. Kapila,et al.  Dietary metabolites derived from gut microbiota: critical modulators of epigenetic changes in mammals , 2017, Nutrition reviews.

[31]  A. Hart,et al.  Clonal evolution of colorectal cancer in IBD , 2017, Nature Reviews Gastroenterology &Hepatology.

[32]  J. Chiang,et al.  Pre‐operative serum albumin level substantially predicts post‐operative morbidity and mortality among patients with colorectal cancer who undergo elective colectomy , 2017, European journal of cancer care.

[33]  David Manrique Vergara,et al.  Short chain fatty acids (butyric acid) and intestinal diseases , 2017, Nutricion hospitalaria.

[34]  Mei Zhang,et al.  Effects of a high fat diet on intestinal microbiota and gastrointestinal diseases , 2016, World journal of gastroenterology.

[35]  Reetta Satokari,et al.  Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp. , 2016, Front. Microbiol..

[36]  Jun Yu,et al.  Fecal Bacteria Act as Novel Biomarkers for Noninvasive Diagnosis of Colorectal Cancer , 2016, Clinical Cancer Research.

[37]  F. Lu,et al.  Effects of different diets on intestinal microbiota and nonalcoholic fatty liver disease development. , 2016, World journal of gastroenterology.

[38]  O. Franco,et al.  Baseline dietary glutamic acid intake and the risk of colorectal cancer: The Rotterdam study , 2016, Cancer.

[39]  S. Ramamoorthy,et al.  Assessment of the Addition of Hypoalbuminemia to ACS-NSQIP Surgical Risk Calculator in Colorectal Cancer , 2016, Medicine.

[40]  Harry J. Flint,et al.  The gut microbiota, bacterial metabolites and colorectal cancer , 2014, Nature Reviews Microbiology.

[41]  S. Rudramurthy,et al.  Ochroconis humicola Coexisting with Esthesioneuroblastoma: An Incidental Coloniser or Allergen? , 2014, Mycopathologia.

[42]  H. Lynch,et al.  Practical genetics of colorectal cancer. , 2013, Chinese clinical oncology.

[43]  I. Thorsdottir,et al.  Nutritional status of cancer patients in chemotherapy; dietary intake, nitrogen balance and screening , 2008, Food & nutrition research.

[44]  Seung-Oe Lim,et al.  Epigenetic changes induced by reactive oxygen species in hepatocellular carcinoma: methylation of the E-cadherin promoter. , 2008, Gastroenterology.

[45]  P. Fürst,et al.  Nitrogen sparing effect of Ornicetil in the immediate postoperative state clinical biochemistry and nitrogen balance. , 1985, Clinical nutrition.